Radiochemical purity and particles number determinations of modified Tc-99m-macroaggregated albumin
Апстракт
A new procedure for the aggregation of human albumin and Tc-99m-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in Tc-99m-MAA were tested. Thus, 85% methanol was used as the mobile phase in paper and ITL chromatography with Whatman N-o 1 and ITLC-SA strips. A system of two solvents (acetone and 1 M NaCl or 0.9% NaCl) was used for 3 MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3 MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency ( GT 98.5%) and percent radiochemical impurities of Tc-99m-MAA. Determination of the particles number in a co...unter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000-350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes.
Кључне речи:
human albumin macroaggregates / Tc-99m-labelling / quality controlИзвор:
Journal of the Serbian Chemical Society, 2002, 67, 8-9, 573-579
DOI: 10.2298/JSC0209573D
ISSN: 0352-5139
WoS: 000178039500004
Scopus: 2-s2.0-0036379504
Колекције
Институција/група
VinčaTY - JOUR AU - Đokić, Divna Đ. AU - Janković, Drina AU - Maksin, Tatjana N. PY - 2002 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2547 AB - A new procedure for the aggregation of human albumin and Tc-99m-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in Tc-99m-MAA were tested. Thus, 85% methanol was used as the mobile phase in paper and ITL chromatography with Whatman N-o 1 and ITLC-SA strips. A system of two solvents (acetone and 1 M NaCl or 0.9% NaCl) was used for 3 MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3 MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency ( GT 98.5%) and percent radiochemical impurities of Tc-99m-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000-350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes. T2 - Journal of the Serbian Chemical Society T1 - Radiochemical purity and particles number determinations of modified Tc-99m-macroaggregated albumin VL - 67 IS - 8-9 SP - 573 EP - 579 DO - 10.2298/JSC0209573D ER -
@article{ author = "Đokić, Divna Đ. and Janković, Drina and Maksin, Tatjana N.", year = "2002", abstract = "A new procedure for the aggregation of human albumin and Tc-99m-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in Tc-99m-MAA were tested. Thus, 85% methanol was used as the mobile phase in paper and ITL chromatography with Whatman N-o 1 and ITLC-SA strips. A system of two solvents (acetone and 1 M NaCl or 0.9% NaCl) was used for 3 MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3 MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency ( GT 98.5%) and percent radiochemical impurities of Tc-99m-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000-350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes.", journal = "Journal of the Serbian Chemical Society", title = "Radiochemical purity and particles number determinations of modified Tc-99m-macroaggregated albumin", volume = "67", number = "8-9", pages = "573-579", doi = "10.2298/JSC0209573D" }
Đokić, D. Đ., Janković, D.,& Maksin, T. N.. (2002). Radiochemical purity and particles number determinations of modified Tc-99m-macroaggregated albumin. in Journal of the Serbian Chemical Society, 67(8-9), 573-579. https://doi.org/10.2298/JSC0209573D
Đokić DĐ, Janković D, Maksin TN. Radiochemical purity and particles number determinations of modified Tc-99m-macroaggregated albumin. in Journal of the Serbian Chemical Society. 2002;67(8-9):573-579. doi:10.2298/JSC0209573D .
Đokić, Divna Đ., Janković, Drina, Maksin, Tatjana N., "Radiochemical purity and particles number determinations of modified Tc-99m-macroaggregated albumin" in Journal of the Serbian Chemical Society, 67, no. 8-9 (2002):573-579, https://doi.org/10.2298/JSC0209573D . .