Rapid detection of the hypertension-associated A1166C polymorphism of the angiotensin II type 1 receptor (AGTR1)
Чланак у часопису
МетаподациПриказ свих података о документу
Screening for polymorphisms in the human type 1 angiotensin II receptor locus (AGTR1) has led to the identification of an A1166C transversion in the Y-untranslated region. This molecular variant, C-1166, has been linked to essential hypertension. We describe here a rapid method for the detection of this point mutation by a simple modification of PCR amplification with allele-specific oligonucleotides (ASO), so as to avoid a hybridization procedure involving either radioactive- or non-radioactive-labeled probes, labeled primers, or restriction typing. The procedure described is convenient for routine clinical laboratory use with manual sample processing and offers the potential for further automation, as well.
Извор:Genetic Testing, 2002, 6, 2, 133-134
ISSN: 1090-6576 (print)