Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells
Нема приказа
Аутори
Ruždijić, SaberaMilošević, Jovan
Popović, Nataša M.
Pešić, Milica
Stojilkovic, M
Kanazir, Selma
Todorović, Danijela V.
Ristić-Fira, Aleksandra
Krstić-Demonacos, Marija
Kanazir, Selma
Rakić, Ljubisav
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and... tiazofurin could provide a promising therapeutic approach for melanoma treatment.
Кључне речи:
apoptosis / tiazofurin / 8-Cl-cAMP / c-fos / c-mycИзвор:
Jugoslovenska Medicinska Biohemija, 2001, 20, 1, 9-18Колекције
Институција/група
VinčaTY - JOUR AU - Ruždijić, Sabera AU - Milošević, Jovan AU - Popović, Nataša M. AU - Pešić, Milica AU - Stojilkovic, M AU - Kanazir, Selma AU - Todorović, Danijela V. AU - Ristić-Fira, Aleksandra AU - Krstić-Demonacos, Marija AU - Kanazir, Selma AU - Rakić, Ljubisav PY - 2001 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2423 AB - Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and tiazofurin could provide a promising therapeutic approach for melanoma treatment. T2 - Jugoslovenska Medicinska Biohemija T1 - Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells VL - 20 IS - 1 SP - 9 EP - 18 UR - https://hdl.handle.net/21.15107/rcub_vinar_2423 ER -
@article{ author = "Ruždijić, Sabera and Milošević, Jovan and Popović, Nataša M. and Pešić, Milica and Stojilkovic, M and Kanazir, Selma and Todorović, Danijela V. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Kanazir, Selma and Rakić, Ljubisav", year = "2001", abstract = "Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and tiazofurin could provide a promising therapeutic approach for melanoma treatment.", journal = "Jugoslovenska Medicinska Biohemija", title = "Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells", volume = "20", number = "1", pages = "9-18", url = "https://hdl.handle.net/21.15107/rcub_vinar_2423" }
Ruždijić, S., Milošević, J., Popović, N. M., Pešić, M., Stojilkovic, M., Kanazir, S., Todorović, D. V., Ristić-Fira, A., Krstić-Demonacos, M., Kanazir, S.,& Rakić, L.. (2001). Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells. in Jugoslovenska Medicinska Biohemija, 20(1), 9-18. https://hdl.handle.net/21.15107/rcub_vinar_2423
Ruždijić S, Milošević J, Popović NM, Pešić M, Stojilkovic M, Kanazir S, Todorović DV, Ristić-Fira A, Krstić-Demonacos M, Kanazir S, Rakić L. Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells. in Jugoslovenska Medicinska Biohemija. 2001;20(1):9-18. https://hdl.handle.net/21.15107/rcub_vinar_2423 .
Ruždijić, Sabera, Milošević, Jovan, Popović, Nataša M., Pešić, Milica, Stojilkovic, M, Kanazir, Selma, Todorović, Danijela V., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Kanazir, Selma, Rakić, Ljubisav, "Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells" in Jugoslovenska Medicinska Biohemija, 20, no. 1 (2001):9-18, https://hdl.handle.net/21.15107/rcub_vinar_2423 .