Effects of estradiol benzoate and progesterone on superoxide dismutase activity in the thymus of rats
Апстракт
The activity of mitochondrial superoxide dismutase (MnSOD) and cytosol superoxide dismutase (CuZnSOD) was measured in corresponding subcellular fractions prepared from the thymi of intact and chronically gonadectomized (GX) rats of both sexes, as well as of GX male and female rats injected subcutaneously with a single dose of 5 mug estradiol benzoate (EB) and/or 2 mg progesterone (P). Animals were sacrificed 2 h or 24 h following hormone treatment. In the females, the activity of MnSOD in the thymus was stable during the estrous cycle and did not change after ovariectomy. Treatment of GX females with estradiol benzoate resulted 2 h later in a significant elevation of MnSOD activity, whereas 24 h later the activity returned back to control values. On the other hand, treatment of GX females with progesterone had no effect on the MnSOD activity. However, combined hormone treatment, in which EB injection preceded progesterone injection by one hour, enhanced the effect on MnSOD activity sim...ilar to that of estradiol benzoate alone. The activity of CuZnSOD in cycling rats was increased in proestrus, whereas removal of the ovaries kept the values at low diestrus and estrus levels. Contrary to MnSOD, CuZnSOD activity did not change after EB treatment of GX females, while progesterone increased the enzyme activity at 2 h and 24 h after hormone treatment. However, combined EB+P treatment proved to be ineffective. In the males, neither MnSOD nor CuZnSOD activity was affected by the removal of testes or by progesterone treatment of GX animals. Only EB injection to GX rats significantly increased CuZnSOD activity 24 h later.
Кључне речи:
superoxide dismutase / thymus / progesterone / estradiol / ratsИзвор:
Physiological Research, 2001, 50, 1, 97-103Колекције
Институција/група
VinčaTY - JOUR AU - Kasapović, Jelena AU - Pajović, Snežana B. AU - Pejić, Snežana AU - Martinović, Jelena PY - 2001 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2422 AB - The activity of mitochondrial superoxide dismutase (MnSOD) and cytosol superoxide dismutase (CuZnSOD) was measured in corresponding subcellular fractions prepared from the thymi of intact and chronically gonadectomized (GX) rats of both sexes, as well as of GX male and female rats injected subcutaneously with a single dose of 5 mug estradiol benzoate (EB) and/or 2 mg progesterone (P). Animals were sacrificed 2 h or 24 h following hormone treatment. In the females, the activity of MnSOD in the thymus was stable during the estrous cycle and did not change after ovariectomy. Treatment of GX females with estradiol benzoate resulted 2 h later in a significant elevation of MnSOD activity, whereas 24 h later the activity returned back to control values. On the other hand, treatment of GX females with progesterone had no effect on the MnSOD activity. However, combined hormone treatment, in which EB injection preceded progesterone injection by one hour, enhanced the effect on MnSOD activity similar to that of estradiol benzoate alone. The activity of CuZnSOD in cycling rats was increased in proestrus, whereas removal of the ovaries kept the values at low diestrus and estrus levels. Contrary to MnSOD, CuZnSOD activity did not change after EB treatment of GX females, while progesterone increased the enzyme activity at 2 h and 24 h after hormone treatment. However, combined EB+P treatment proved to be ineffective. In the males, neither MnSOD nor CuZnSOD activity was affected by the removal of testes or by progesterone treatment of GX animals. Only EB injection to GX rats significantly increased CuZnSOD activity 24 h later. T2 - Physiological Research T1 - Effects of estradiol benzoate and progesterone on superoxide dismutase activity in the thymus of rats VL - 50 IS - 1 SP - 97 EP - 103 UR - https://hdl.handle.net/21.15107/rcub_vinar_2422 ER -
@article{ author = "Kasapović, Jelena and Pajović, Snežana B. and Pejić, Snežana and Martinović, Jelena", year = "2001", abstract = "The activity of mitochondrial superoxide dismutase (MnSOD) and cytosol superoxide dismutase (CuZnSOD) was measured in corresponding subcellular fractions prepared from the thymi of intact and chronically gonadectomized (GX) rats of both sexes, as well as of GX male and female rats injected subcutaneously with a single dose of 5 mug estradiol benzoate (EB) and/or 2 mg progesterone (P). Animals were sacrificed 2 h or 24 h following hormone treatment. In the females, the activity of MnSOD in the thymus was stable during the estrous cycle and did not change after ovariectomy. Treatment of GX females with estradiol benzoate resulted 2 h later in a significant elevation of MnSOD activity, whereas 24 h later the activity returned back to control values. On the other hand, treatment of GX females with progesterone had no effect on the MnSOD activity. However, combined hormone treatment, in which EB injection preceded progesterone injection by one hour, enhanced the effect on MnSOD activity similar to that of estradiol benzoate alone. The activity of CuZnSOD in cycling rats was increased in proestrus, whereas removal of the ovaries kept the values at low diestrus and estrus levels. Contrary to MnSOD, CuZnSOD activity did not change after EB treatment of GX females, while progesterone increased the enzyme activity at 2 h and 24 h after hormone treatment. However, combined EB+P treatment proved to be ineffective. In the males, neither MnSOD nor CuZnSOD activity was affected by the removal of testes or by progesterone treatment of GX animals. Only EB injection to GX rats significantly increased CuZnSOD activity 24 h later.", journal = "Physiological Research", title = "Effects of estradiol benzoate and progesterone on superoxide dismutase activity in the thymus of rats", volume = "50", number = "1", pages = "97-103", url = "https://hdl.handle.net/21.15107/rcub_vinar_2422" }
Kasapović, J., Pajović, S. B., Pejić, S.,& Martinović, J.. (2001). Effects of estradiol benzoate and progesterone on superoxide dismutase activity in the thymus of rats. in Physiological Research, 50(1), 97-103. https://hdl.handle.net/21.15107/rcub_vinar_2422
Kasapović J, Pajović SB, Pejić S, Martinović J. Effects of estradiol benzoate and progesterone on superoxide dismutase activity in the thymus of rats. in Physiological Research. 2001;50(1):97-103. https://hdl.handle.net/21.15107/rcub_vinar_2422 .
Kasapović, Jelena, Pajović, Snežana B., Pejić, Snežana, Martinović, Jelena, "Effects of estradiol benzoate and progesterone on superoxide dismutase activity in the thymus of rats" in Physiological Research, 50, no. 1 (2001):97-103, https://hdl.handle.net/21.15107/rcub_vinar_2422 .