Kinetics of thyroxine (T-4) and triiodothyronine (T-3) transport in the isolated rat heart

Abstract

The dynamics and kinetics of thyroid hormone transport in the isolated rat heart were examined using the modified unidirectional paired tracer dilution method. The uptake of I-125-thyroxine (I-115-T-4) and I-125-triiodothyronine (I-125-T-3) from the extracellular space into heart cells was measured relative to the extracellular space marker H-3-mannitol. The thyroid hormone maximal uptake was 54.4 % for I-125-T-4 and 52.15 % for I-125-T-3. The thyroid hormone net uptake was 25.69 % for I-125-T-4 and 25.49 % for I-125-T-3. Backflux from the intracellular space was 53.17 % for I-125-T-4 and 61.59 % for I-125-T-3. In the presence of unlabelled thyroid hormones, I-125-T-4 and I-125-T-3 maximal uptakes were reduced from 10.1 to 59.74 % and from 34.6 to 65.3 %, respectively, depending on the concentration of the unlabelled hormone, suggesting a saturable mechanism of the thyroid hormone uptake by the heart cells, with K-m(T4) = 105.46 muM and the maximal rate of I-125-thyroid hormone flax from the extracellular space to heart cells (V-max(T4))= 177.84 nM min(-1) for I-125-T-4 uptake, and K-m(T3) = 80.0 muM and V-max(T3) = 118.5 nM min(-1) for I-125-T-3 uptake.