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dc.creatorJoksić, Gordana
dc.creatorStankovic, M
dc.creatorVasić, Vesna M.
dc.creatorČakar, Mira M.
dc.creatorJokanović, Milan
dc.date.accessioned2018-03-01T18:54:19Z
dc.date.available2018-03-01T18:54:19Z
dc.date.issued2000
dc.identifier.issn0028-2685 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/2383
dc.description.abstractCytotoxic effects of the antiviremic drug ribavirin (1-beta -D-ribofuranosyl-1,2,4-triazole-3-carboxamide) were evaluated in vitro measuring micronucleus formation and cell proliferation kinetics in whole blood cultures employing cytokinesis block (CB) micronucleus test. The cells were exposed to ribavirin doses ranging from 0.05-0.65 mu mol/ml at three different incubation times. The frequency of micronuclei in treated samples demonstrated relatively low ability of ribavirin to induce micronuclei. However, the lowest concentration of ribavirin markedly changed the frequency of mononucleated and multinucleated cells, particularly binucleated ones, which declined significantly. The decline in the frequency of binucleate cells was followed with accumulation of greater number of mononucleate cells. Decreased proliferation potential of lymphocytes treated with ribavirin indicates that cells are arrested prior to metaphase. The present investigation showed that ribavirin is capable to induce a delay in cellular proliferation at all the doses assayed. The study demonstrated that CB micronucleus assay is simple and rapid method that can be used to assess toxic effect of drugs in vitro.en
dc.rightsrestrictedAccessen
dc.sourceNeoplasmaen
dc.subjectribavirinen
dc.subjectmicronucleien
dc.subjectproliferation potentialen
dc.subjecthuman lymphocytesen
dc.subjecttoxicityen
dc.titleInfluence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytesen
dc.typearticleen
dcterms.abstractЦакар, М; Јокановиц, М; Станковиц, М; Јоксић Гордана; Васић Весна М;
dc.citation.volume47
dc.citation.issue5
dc.citation.spage283
dc.citation.epage287
dc.identifier.wos000089988200005
dc.citation.rankM23
dc.identifier.pmid11130244


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