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dc.creatorKaradzic, Ivana
dc.creatorVucic, Vesna
dc.creatorJokanović, Vukoman R.
dc.creatorDebeljak-Martacic, Jasmina
dc.creatorMarkovic, Dejan
dc.creatorPetrovic, Snjezana
dc.creatorGlibetic, Marija
dc.date.accessioned2018-03-01T15:29:59Z
dc.date.available2018-03-01T15:29:59Z
dc.date.issued2015
dc.identifier.issn1549-3296 (print)
dc.identifier.issn1552-4965 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/235
dc.description.abstractThe aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41030/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172026/RS//
dc.rightsrestrictedAccessen
dc.sourceJournal of Biomedical Materials Research. Part Aen
dc.subjectdental pulp mesenchymal stem cellsen
dc.subjecthydroxyapatiteen
dc.subjectscaffoldsen
dc.subjectosteogenic differentiationen
dc.subjectbone tissue engineeringen
dc.titleEffects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cellsen
dc.typearticleen
dcterms.abstractЈокановић Вукоман Р.; Дебељак-Мартациц, Јасмина; Вуциц, Весна; Карадзиц, Ивана; Глибетиц, Марија; Петровиц, Сњезана; Марковиц, Дејан;
dc.citation.volume103
dc.citation.issue1
dc.citation.spage350
dc.citation.epage357
dc.identifier.wos000345572100039
dc.identifier.doi10.1002/jbm.a.35180
dc.citation.rankM21
dc.identifier.pmid24665062
dc.identifier.scopus2-s2.0-84912531491


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