Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells
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2015
Authors
Karadzic, IvanaVučić, Vesna
Jokanović, Vukoman R.
Debeljak-Martačić, Jasmina
Marković, Dejan
Petrović, Snježana
Glibetić, Marija
Article (Published version)
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The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiat...ion and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.
Keywords:
dental pulp mesenchymal stem cells / hydroxyapatite / scaffolds / osteogenic differentiation / bone tissue engineeringSource:
Journal of Biomedical Materials Research. Part A, 2015, 103, 1, 350-357Funding / projects:
- Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia (RS-41030)
- Chemical and structural designing of nanomaterials for application in medicine and tissue engineering (RS-172026)
DOI: 10.1002/jbm.a.35180
ISSN: 1549-3296; 1552-4965
PubMed: 24665062
WoS: 000345572100039
Scopus: 2-s2.0-84912531491
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VinčaTY - JOUR AU - Karadzic, Ivana AU - Vučić, Vesna AU - Jokanović, Vukoman R. AU - Debeljak-Martačić, Jasmina AU - Marković, Dejan AU - Petrović, Snježana AU - Glibetić, Marija PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/235 AB - The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015. T2 - Journal of Biomedical Materials Research. Part A T1 - Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells VL - 103 IS - 1 SP - 350 EP - 357 DO - 10.1002/jbm.a.35180 ER -
@article{ author = "Karadzic, Ivana and Vučić, Vesna and Jokanović, Vukoman R. and Debeljak-Martačić, Jasmina and Marković, Dejan and Petrović, Snježana and Glibetić, Marija", year = "2015", abstract = "The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.", journal = "Journal of Biomedical Materials Research. Part A", title = "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells", volume = "103", number = "1", pages = "350-357", doi = "10.1002/jbm.a.35180" }
Karadzic, I., Vučić, V., Jokanović, V. R., Debeljak-Martačić, J., Marković, D., Petrović, S.,& Glibetić, M.. (2015). Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A, 103(1), 350-357. https://doi.org/10.1002/jbm.a.35180
Karadzic I, Vučić V, Jokanović VR, Debeljak-Martačić J, Marković D, Petrović S, Glibetić M. Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A. 2015;103(1):350-357. doi:10.1002/jbm.a.35180 .
Karadzic, Ivana, Vučić, Vesna, Jokanović, Vukoman R., Debeljak-Martačić, Jasmina, Marković, Dejan, Petrović, Snježana, Glibetić, Marija, "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells" in Journal of Biomedical Materials Research. Part A, 103, no. 1 (2015):350-357, https://doi.org/10.1002/jbm.a.35180 . .