Show simple item record

dc.creatorVuckovic, M
dc.creatorRadojcic, M
dc.creatorMilosavljevic, BH
dc.date.accessioned2018-03-01T18:49:54Z
dc.date.available2018-03-01T18:49:54Z
dc.date.issued2000
dc.identifier.issn0352-5139
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/2332
dc.description.abstractThe analysis of the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) data, combined with the comparison of viscosity data corresponding to the, thin fraction of hell egg white and 2 mM purefied ovalbumin solution, showed that the thin fraction is a protein solution. Dissolution of the thick fraction of hen egg white in SDS and urea solutions followed by SDS-PAGE, in the presence or absence of P-mercaptoethanol, revealed that it is a protein gel. It was also found that the gel structure consists of the three-dimensional ovomucine-ovomucoid network (matrix) held together by SS bridges which captures the rest of the proteins (ovalbumin, conalbumin, etc). The captured proteins can also be interconnected by S-S bridges thus forming agglomerates or conglomerates, but they are predominantly held by weak electrostatic interactions, as demonstrated by the washing out and dissolving experiments. The matrix structure does not prevent denaturation of the captured proteins as indecated by the 50% decrease in turbidity following gel swelling by the addition of 1 part of an 8 M urea solution to 9 parts of the gel.en
dc.rightsopenAccessen
dc.sourceJournal of the Serbian Chemical Societyen
dc.subjectproteinen
dc.subjectgelen
dc.subjectdisulfide bonden
dc.subjectbound wateren
dc.subjectdenaturationen
dc.titleInter-protein bonding and other molecular interactions in hen egg whiteen
dc.typearticleen
dcterms.abstractРадојциц, М; Милосављевиц, БХ; Вуцковиц, М;
dc.citation.volume65
dc.citation.issue3
dc.citation.spage157
dc.citation.epage166
dc.identifier.wos000086052500002
dc.citation.rankM23


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record