Determination of the critical molar mass of ovalbumin oligomers degraded by ultrasound
Апстракт
An experimental method has been developed which enables the determination of the critical molar mass (Mmc) of ovalbumin oligomers degraded by ultrasound of known frequency. To test the validity of the Mmc postulate, a series of ovalbumin oligomers was prepared by the radiolytic cross-linking of 1% solutions of ovalbumin monomer dissolved in 50 mM Na/K-phosphate buffer pH 7.0 saturated with N2O. Under these conditions, irradiation with 5 kGy from a Co-60 source, yielded ovalbumin dimers, trimers, tetramers, and higher order oligomers. On the basis of the results obtained with the ovalbumin oligomers, it was concluded that for ultrasound of 23 kHz frequency and 5 mu m amplitude, the Mmc was 274000 +/- 14000 g/mol. Our results confirmed that the two postulates in the chemistry of polymer degradation by ultrasound are valid when ovalbumin oligomers are used as substrates, i.e., (1) that the higher the molar mass of the original macromolecule, the faster is its degradation rate, and (2) tha...t a lower molar mass limit (LMmL) exists below which the macromolecules are resistent to further degradation.
Кључне речи:
ovalbumin / ultrasound / critical molar massИзвор:
Journal of the Serbian Chemical Society, 2000, 65, 2, 123-130Колекције
Институција/група
VinčaTY - JOUR AU - Filipović, Dragana AU - Radojčić, Marija AU - Milosavljević, B.H. PY - 2000 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2328 AB - An experimental method has been developed which enables the determination of the critical molar mass (Mmc) of ovalbumin oligomers degraded by ultrasound of known frequency. To test the validity of the Mmc postulate, a series of ovalbumin oligomers was prepared by the radiolytic cross-linking of 1% solutions of ovalbumin monomer dissolved in 50 mM Na/K-phosphate buffer pH 7.0 saturated with N2O. Under these conditions, irradiation with 5 kGy from a Co-60 source, yielded ovalbumin dimers, trimers, tetramers, and higher order oligomers. On the basis of the results obtained with the ovalbumin oligomers, it was concluded that for ultrasound of 23 kHz frequency and 5 mu m amplitude, the Mmc was 274000 +/- 14000 g/mol. Our results confirmed that the two postulates in the chemistry of polymer degradation by ultrasound are valid when ovalbumin oligomers are used as substrates, i.e., (1) that the higher the molar mass of the original macromolecule, the faster is its degradation rate, and (2) that a lower molar mass limit (LMmL) exists below which the macromolecules are resistent to further degradation. T2 - Journal of the Serbian Chemical Society T1 - Determination of the critical molar mass of ovalbumin oligomers degraded by ultrasound VL - 65 IS - 2 SP - 123 EP - 130 UR - https://hdl.handle.net/21.15107/rcub_vinar_2328 ER -
@article{ author = "Filipović, Dragana and Radojčić, Marija and Milosavljević, B.H.", year = "2000", abstract = "An experimental method has been developed which enables the determination of the critical molar mass (Mmc) of ovalbumin oligomers degraded by ultrasound of known frequency. To test the validity of the Mmc postulate, a series of ovalbumin oligomers was prepared by the radiolytic cross-linking of 1% solutions of ovalbumin monomer dissolved in 50 mM Na/K-phosphate buffer pH 7.0 saturated with N2O. Under these conditions, irradiation with 5 kGy from a Co-60 source, yielded ovalbumin dimers, trimers, tetramers, and higher order oligomers. On the basis of the results obtained with the ovalbumin oligomers, it was concluded that for ultrasound of 23 kHz frequency and 5 mu m amplitude, the Mmc was 274000 +/- 14000 g/mol. Our results confirmed that the two postulates in the chemistry of polymer degradation by ultrasound are valid when ovalbumin oligomers are used as substrates, i.e., (1) that the higher the molar mass of the original macromolecule, the faster is its degradation rate, and (2) that a lower molar mass limit (LMmL) exists below which the macromolecules are resistent to further degradation.", journal = "Journal of the Serbian Chemical Society", title = "Determination of the critical molar mass of ovalbumin oligomers degraded by ultrasound", volume = "65", number = "2", pages = "123-130", url = "https://hdl.handle.net/21.15107/rcub_vinar_2328" }
Filipović, D., Radojčić, M.,& Milosavljević, B.H.. (2000). Determination of the critical molar mass of ovalbumin oligomers degraded by ultrasound. in Journal of the Serbian Chemical Society, 65(2), 123-130. https://hdl.handle.net/21.15107/rcub_vinar_2328
Filipović D, Radojčić M, Milosavljević B. Determination of the critical molar mass of ovalbumin oligomers degraded by ultrasound. in Journal of the Serbian Chemical Society. 2000;65(2):123-130. https://hdl.handle.net/21.15107/rcub_vinar_2328 .
Filipović, Dragana, Radojčić, Marija, Milosavljević, B.H., "Determination of the critical molar mass of ovalbumin oligomers degraded by ultrasound" in Journal of the Serbian Chemical Society, 65, no. 2 (2000):123-130, https://hdl.handle.net/21.15107/rcub_vinar_2328 .