Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex
Abstract
Social isolation produce various neurochemical alterations in monoamines pathways of the brain. In order to assess the effect of individual housing of adult rats on neurochemical substrates and lateralization in the right and left medial prefrontal cortex (mPFC), we measured dopamine and noradrenaline stores, protein levels of tyrosine-hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2), by Western blot analysis. Individual housing produced no significant changes in dopamine and noradrenaline content, whereas levels of TH and NET were elevated in the right and left mPFC. On the other hand, VMAT 2 protein was reduced in the right mPFC and increased in the left mPFC. The results indicate an increased synthetic and reuptake capacity of noradrenergic terminals in the right and left mPFC and decreased translocation monoamines from the cytoplasm into the intracellular storage vesicles of neurons in the right mPFC.
Keywords:
isolation stress / medial prefrontal cortex / catecholamine / noradrenaline transporters / rats / tyrosine hydroxylaseSource:
Neurochemical Journal, 2014, 8, 4, 277-281Funding / projects:
- Molecular mechanisms of cellular responses on pathological changes in central neuronal system and peripheral organs of mammals (RS-MESTD-Basic Research (BR or ON)-173044)
DOI: 10.1134/S1819712414040084
ISSN: 1819-7124; 1819-7132
WoS: 000345424300007
Scopus: 2-s2.0-84911970812
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VinčaTY - JOUR AU - Spasojević, Nataša AU - Jovanović, Predrag AU - Stefanović, Bojana AU - Dronjak, Slađana PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/227 AB - Social isolation produce various neurochemical alterations in monoamines pathways of the brain. In order to assess the effect of individual housing of adult rats on neurochemical substrates and lateralization in the right and left medial prefrontal cortex (mPFC), we measured dopamine and noradrenaline stores, protein levels of tyrosine-hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2), by Western blot analysis. Individual housing produced no significant changes in dopamine and noradrenaline content, whereas levels of TH and NET were elevated in the right and left mPFC. On the other hand, VMAT 2 protein was reduced in the right mPFC and increased in the left mPFC. The results indicate an increased synthetic and reuptake capacity of noradrenergic terminals in the right and left mPFC and decreased translocation monoamines from the cytoplasm into the intracellular storage vesicles of neurons in the right mPFC. T2 - Neurochemical Journal T1 - Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex VL - 8 IS - 4 SP - 277 EP - 281 DO - 10.1134/S1819712414040084 ER -
@article{ author = "Spasojević, Nataša and Jovanović, Predrag and Stefanović, Bojana and Dronjak, Slađana", year = "2014", abstract = "Social isolation produce various neurochemical alterations in monoamines pathways of the brain. In order to assess the effect of individual housing of adult rats on neurochemical substrates and lateralization in the right and left medial prefrontal cortex (mPFC), we measured dopamine and noradrenaline stores, protein levels of tyrosine-hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2), by Western blot analysis. Individual housing produced no significant changes in dopamine and noradrenaline content, whereas levels of TH and NET were elevated in the right and left mPFC. On the other hand, VMAT 2 protein was reduced in the right mPFC and increased in the left mPFC. The results indicate an increased synthetic and reuptake capacity of noradrenergic terminals in the right and left mPFC and decreased translocation monoamines from the cytoplasm into the intracellular storage vesicles of neurons in the right mPFC.", journal = "Neurochemical Journal", title = "Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex", volume = "8", number = "4", pages = "277-281", doi = "10.1134/S1819712414040084" }
Spasojević, N., Jovanović, P., Stefanović, B.,& Dronjak, S.. (2014). Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex. in Neurochemical Journal, 8(4), 277-281. https://doi.org/10.1134/S1819712414040084
Spasojević N, Jovanović P, Stefanović B, Dronjak S. Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex. in Neurochemical Journal. 2014;8(4):277-281. doi:10.1134/S1819712414040084 .
Spasojević, Nataša, Jovanović, Predrag, Stefanović, Bojana, Dronjak, Slađana, "Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex" in Neurochemical Journal, 8, no. 4 (2014):277-281, https://doi.org/10.1134/S1819712414040084 . .