Properties of Mg2+-ATPase in rat brain synaptic plasma membranes
Само за регистроване кориснике
1998
Аутори
Nedeljković, N.Nikezić, Gordana S.
Horvat, Anica
Peković, Sanja
Stojiljković, Mirjana
Martinović, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In the present study distribution and enzymatic properties of ecto-Mg2+-ATPase were determined in synaptic plasma membrane (SPM) preparations isolated from the hippocampus, caudate nucleus and whole brains of female rats. Western blot analysis using anti-ecto-Mg2+-ATPase antibody revealed the association of Mg2+-ATPase with SPM prepared from all the three brain sources, yet the enzyme was most abundant in caudate nucleus membranes, being 30% and 22% more abundant than in the hippocampal and whole brain tissue SPM, respectively. The evidence is also presented that kinetic properties of the brain Mg2+-ATPase are not under the control of circulating sex steroids. It was confirmed that the enzyme is activated by millimolar concentrations of Mg2+ and that it cannot be effectively inhibited by known ATPase inhibitors. The most pronounced differences in kinetic properties observed were 2.5 fold higher apparent affinity for ATP and 59% higher specific activity of Mg2+-ATPase of the caudate nuc...leus as compared with the enzyme from the hippocampus. On the other hand, the apparent enzyme affinity for Mg2+ was almost equal in all SPM preparations tested. Taken together, our results show that ecto-Mg2+-ATPase is not uniformly distributed and differs in respect to affinity for ATP in rat brain regions, thus indicating its substantial role in the process of signal transduction via controlling the levels of extracellular ATP.
Кључне речи:
ecto-Mg2+-ATPase / synaptic plasma membranes / hippocampus / caudate nucleusИзвор:
General Physiology and Biophysics, 1998, 17, 1, 3-13
ISSN: 0231-5882; 1338-4325
PubMed: 9675552
WoS: 000073793000001
Scopus: 2-s2.0-0032013976
Колекције
Институција/група
VinčaTY - JOUR AU - Nedeljković, N. AU - Nikezić, Gordana S. AU - Horvat, Anica AU - Peković, Sanja AU - Stojiljković, Mirjana AU - Martinović, Jelena PY - 1998 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2145 AB - In the present study distribution and enzymatic properties of ecto-Mg2+-ATPase were determined in synaptic plasma membrane (SPM) preparations isolated from the hippocampus, caudate nucleus and whole brains of female rats. Western blot analysis using anti-ecto-Mg2+-ATPase antibody revealed the association of Mg2+-ATPase with SPM prepared from all the three brain sources, yet the enzyme was most abundant in caudate nucleus membranes, being 30% and 22% more abundant than in the hippocampal and whole brain tissue SPM, respectively. The evidence is also presented that kinetic properties of the brain Mg2+-ATPase are not under the control of circulating sex steroids. It was confirmed that the enzyme is activated by millimolar concentrations of Mg2+ and that it cannot be effectively inhibited by known ATPase inhibitors. The most pronounced differences in kinetic properties observed were 2.5 fold higher apparent affinity for ATP and 59% higher specific activity of Mg2+-ATPase of the caudate nucleus as compared with the enzyme from the hippocampus. On the other hand, the apparent enzyme affinity for Mg2+ was almost equal in all SPM preparations tested. Taken together, our results show that ecto-Mg2+-ATPase is not uniformly distributed and differs in respect to affinity for ATP in rat brain regions, thus indicating its substantial role in the process of signal transduction via controlling the levels of extracellular ATP. T2 - General Physiology and Biophysics T1 - Properties of Mg2+-ATPase in rat brain synaptic plasma membranes VL - 17 IS - 1 SP - 3 EP - 13 UR - https://hdl.handle.net/21.15107/rcub_vinar_2145 ER -
@article{ author = "Nedeljković, N. and Nikezić, Gordana S. and Horvat, Anica and Peković, Sanja and Stojiljković, Mirjana and Martinović, Jelena", year = "1998", abstract = "In the present study distribution and enzymatic properties of ecto-Mg2+-ATPase were determined in synaptic plasma membrane (SPM) preparations isolated from the hippocampus, caudate nucleus and whole brains of female rats. Western blot analysis using anti-ecto-Mg2+-ATPase antibody revealed the association of Mg2+-ATPase with SPM prepared from all the three brain sources, yet the enzyme was most abundant in caudate nucleus membranes, being 30% and 22% more abundant than in the hippocampal and whole brain tissue SPM, respectively. The evidence is also presented that kinetic properties of the brain Mg2+-ATPase are not under the control of circulating sex steroids. It was confirmed that the enzyme is activated by millimolar concentrations of Mg2+ and that it cannot be effectively inhibited by known ATPase inhibitors. The most pronounced differences in kinetic properties observed were 2.5 fold higher apparent affinity for ATP and 59% higher specific activity of Mg2+-ATPase of the caudate nucleus as compared with the enzyme from the hippocampus. On the other hand, the apparent enzyme affinity for Mg2+ was almost equal in all SPM preparations tested. Taken together, our results show that ecto-Mg2+-ATPase is not uniformly distributed and differs in respect to affinity for ATP in rat brain regions, thus indicating its substantial role in the process of signal transduction via controlling the levels of extracellular ATP.", journal = "General Physiology and Biophysics", title = "Properties of Mg2+-ATPase in rat brain synaptic plasma membranes", volume = "17", number = "1", pages = "3-13", url = "https://hdl.handle.net/21.15107/rcub_vinar_2145" }
Nedeljković, N., Nikezić, G. S., Horvat, A., Peković, S., Stojiljković, M.,& Martinović, J.. (1998). Properties of Mg2+-ATPase in rat brain synaptic plasma membranes. in General Physiology and Biophysics, 17(1), 3-13. https://hdl.handle.net/21.15107/rcub_vinar_2145
Nedeljković N, Nikezić GS, Horvat A, Peković S, Stojiljković M, Martinović J. Properties of Mg2+-ATPase in rat brain synaptic plasma membranes. in General Physiology and Biophysics. 1998;17(1):3-13. https://hdl.handle.net/21.15107/rcub_vinar_2145 .
Nedeljković, N., Nikezić, Gordana S., Horvat, Anica, Peković, Sanja, Stojiljković, Mirjana, Martinović, Jelena, "Properties of Mg2+-ATPase in rat brain synaptic plasma membranes" in General Physiology and Biophysics, 17, no. 1 (1998):3-13, https://hdl.handle.net/21.15107/rcub_vinar_2145 .