Show simple item record

dc.creatorPekovic, S
dc.creatorNedeljkovic, N
dc.creatorNikezic, G
dc.creatorHorvat, Anica
dc.creatorStojiljković, Mirjana
dc.creatorRakic, L
dc.creatorMartinović, Jelena
dc.date.accessioned2018-03-01T18:22:52Z
dc.date.available2018-03-01T18:22:52Z
dc.date.issued1997
dc.identifier.issn0231-5882 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/2098
dc.description.abstractThe activities and basic enzymatic properties of Na,K-ATPase were examined in synaptosomal plasma membranes (SPM) prepared from rat hippocampus and striatum. A kinetic analysis showed profound differences in apparent affinities for ATP (K-m) between hippocampal (1.21 mmol/l) and striatal (0.76 mmol/l) enzyme preparations, as well as in the corresponding V-max values. However, physiological efficiencies were almost the same. The complex pattern of dose-response curves to ouabain indicated the presence of two high-affinity forms of Na,K-ATPase in the striatum (very high-:K-i = 3.73 x 10(-8) mol/l and high-:K-i = 4.21 x 10(-5) mol/l), and one high affinity form in the hippocampus (K-i = 6.6 x 10(-7) mol/l). In addition, both SPM preparations contained one low affinity form with similar K-i. The very high-affinity form had positive cooperativity for ouabain inhibition of Na,K-ATPase activity, in contrast to high- and low-affinity forms, which exhibited negative cooperativity. The respective contributions of ouabain-sensitive forms to the total activity were estimated as 22%, 46%, 19% for the striatum and 36%, 45% for the hippocampus. These data clearly demonstrate striking differences in kinetic properties of the hippocampal and striatal Na,K-ATPase that may be due to the isoenzyme diversity and adaptation to specific physiological demands of the examined rat brain regions.en
dc.rightsrestrictedAccessen
dc.sourceGeneral Physiology and Biophysicsen
dc.subjectNa,K-ATPaseen
dc.subjectsynaptic plasma membranesen
dc.subjecthippocampusen
dc.subjectstriatumen
dc.subjectraten
dc.titleBiochemical characterization of the hippocampal and striatal Na,K-ATPase reveals striking differences in kinetic propertiesen
dc.typearticleen
dcterms.abstractПековиц, С; Недељковиц, Н; Никезиц, Г; Ракиц, Л; Стојиљковиц, М; Хорват, Aница; Мартиновић Јелена;
dc.citation.volume16
dc.citation.issue3
dc.citation.spage227
dc.citation.epage240
dc.identifier.wosA1997YJ03200003
dc.identifier.pmid9452945


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record