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Biochemical characterization of the hippocampal and striatal Na,K-ATPase reveals striking differences in kinetic properties
dc.creator | Peković, Sanja | |
dc.creator | Nedeljković, N. | |
dc.creator | Nikezić, Gordana S. | |
dc.creator | Horvat, Anica | |
dc.creator | Stojiljković, Mirjana | |
dc.creator | Rakić, Ljubisav | |
dc.creator | Martinović, Jelena | |
dc.date.accessioned | 2018-03-01T18:22:52Z | |
dc.date.available | 2018-03-01T18:22:52Z | |
dc.date.issued | 1997 | |
dc.identifier.issn | 0231-5882 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/2098 | |
dc.description.abstract | The activities and basic enzymatic properties of Na,K-ATPase were examined in synaptosomal plasma membranes (SPM) prepared from rat hippocampus and striatum. A kinetic analysis showed profound differences in apparent affinities for ATP (K-m) between hippocampal (1.21 mmol/l) and striatal (0.76 mmol/l) enzyme preparations, as well as in the corresponding V-max values. However, physiological efficiencies were almost the same. The complex pattern of dose-response curves to ouabain indicated the presence of two high-affinity forms of Na,K-ATPase in the striatum (very high-:K-i = 3.73 x 10(-8) mol/l and high-:K-i = 4.21 x 10(-5) mol/l), and one high affinity form in the hippocampus (K-i = 6.6 x 10(-7) mol/l). In addition, both SPM preparations contained one low affinity form with similar K-i. The very high-affinity form had positive cooperativity for ouabain inhibition of Na,K-ATPase activity, in contrast to high- and low-affinity forms, which exhibited negative cooperativity. The respective contributions of ouabain-sensitive forms to the total activity were estimated as 22%, 46%, 19% for the striatum and 36%, 45% for the hippocampus. These data clearly demonstrate striking differences in kinetic properties of the hippocampal and striatal Na,K-ATPase that may be due to the isoenzyme diversity and adaptation to specific physiological demands of the examined rat brain regions. | en |
dc.rights | restrictedAccess | en |
dc.source | General Physiology and Biophysics | en |
dc.subject | Na,K-ATPase | en |
dc.subject | synaptic plasma membranes | en |
dc.subject | hippocampus | en |
dc.subject | striatum | en |
dc.subject | rat | en |
dc.title | Biochemical characterization of the hippocampal and striatal Na,K-ATPase reveals striking differences in kinetic properties | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Пековиц, С; Недељковиц, Н; Никезиц, Г; Ракиц, Л; Стојиљковиц, М; Хорват, Aница; Мартиновић Јелена; | |
dc.citation.volume | 16 | |
dc.citation.issue | 3 | |
dc.citation.spage | 227 | |
dc.citation.epage | 240 | |
dc.identifier.wos | A1997YJ03200003 | |
dc.identifier.pmid | 9452945 | |
dc.type.version | publishedVersion | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_vinar_2098 |