Codominant regulation of keratin gene expression by cell surface receptors and nuclear receptors
Апстракт
Epidermal keratinocytes are subject to a large variety of signals that modulate their differentiation in health and their activation in disease. Hormones and vitamins, which act via nuclear receptors, affect the differentiation process, whereas growth factors and cytokines, which act via cell surface receptors, affect keratinocyte activation and related events. Using expression of keratin genes as markers for keratinocyte phenotype, we examined the interaction between the nuclear receptor and cell surface receptor pathways. We expected to find dominance of one of the pathways. Surprisingly, we found that the two pathways are co-dominant. Specifically, while EGF induces expression of K6 and K16 keratin genes, retinoic acid suppresses their expression, and when both mediators are present simultaneously, the level of expression is intermediate, a product of both signals. Similar codominant effects were found on other keratin genes using interferon gamma, TGF beta, and thyroid hormone sign...aling molecules. These codominant effects are specific only for genes that are regulated by both pathways. Our results suggest that a judicious combination of hormones, vitamins, growth factors, and cytokines may be used to target specific expression of appropriate genes in the treatment of human epidermal diseases. (C) 1996 Academic Press, Inc.
Извор:
Experimental Cell Research, 1996, 224, 1, 96-102Финансирање / пројекти:
- NIAMS NIH HHS [AR40522, AR30682, AR39176]
DOI: 10.1006/excr.1996.0115
ISSN: 0014-4827
PubMed: 8612697
WoS: A1996UH25100011
Scopus: 2-s2.0-17644441891
Колекције
Институција/група
VinčaTY - JOUR AU - Tomić-Canić, Marjana AU - Freedberg, IM AU - Blumenberg, M PY - 1996 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1976 AB - Epidermal keratinocytes are subject to a large variety of signals that modulate their differentiation in health and their activation in disease. Hormones and vitamins, which act via nuclear receptors, affect the differentiation process, whereas growth factors and cytokines, which act via cell surface receptors, affect keratinocyte activation and related events. Using expression of keratin genes as markers for keratinocyte phenotype, we examined the interaction between the nuclear receptor and cell surface receptor pathways. We expected to find dominance of one of the pathways. Surprisingly, we found that the two pathways are co-dominant. Specifically, while EGF induces expression of K6 and K16 keratin genes, retinoic acid suppresses their expression, and when both mediators are present simultaneously, the level of expression is intermediate, a product of both signals. Similar codominant effects were found on other keratin genes using interferon gamma, TGF beta, and thyroid hormone signaling molecules. These codominant effects are specific only for genes that are regulated by both pathways. Our results suggest that a judicious combination of hormones, vitamins, growth factors, and cytokines may be used to target specific expression of appropriate genes in the treatment of human epidermal diseases. (C) 1996 Academic Press, Inc. T2 - Experimental Cell Research T1 - Codominant regulation of keratin gene expression by cell surface receptors and nuclear receptors VL - 224 IS - 1 SP - 96 EP - 102 DO - 10.1006/excr.1996.0115 ER -
@article{ author = "Tomić-Canić, Marjana and Freedberg, IM and Blumenberg, M", year = "1996", abstract = "Epidermal keratinocytes are subject to a large variety of signals that modulate their differentiation in health and their activation in disease. Hormones and vitamins, which act via nuclear receptors, affect the differentiation process, whereas growth factors and cytokines, which act via cell surface receptors, affect keratinocyte activation and related events. Using expression of keratin genes as markers for keratinocyte phenotype, we examined the interaction between the nuclear receptor and cell surface receptor pathways. We expected to find dominance of one of the pathways. Surprisingly, we found that the two pathways are co-dominant. Specifically, while EGF induces expression of K6 and K16 keratin genes, retinoic acid suppresses their expression, and when both mediators are present simultaneously, the level of expression is intermediate, a product of both signals. Similar codominant effects were found on other keratin genes using interferon gamma, TGF beta, and thyroid hormone signaling molecules. These codominant effects are specific only for genes that are regulated by both pathways. Our results suggest that a judicious combination of hormones, vitamins, growth factors, and cytokines may be used to target specific expression of appropriate genes in the treatment of human epidermal diseases. (C) 1996 Academic Press, Inc.", journal = "Experimental Cell Research", title = "Codominant regulation of keratin gene expression by cell surface receptors and nuclear receptors", volume = "224", number = "1", pages = "96-102", doi = "10.1006/excr.1996.0115" }
Tomić-Canić, M., Freedberg, I.,& Blumenberg, M.. (1996). Codominant regulation of keratin gene expression by cell surface receptors and nuclear receptors. in Experimental Cell Research, 224(1), 96-102. https://doi.org/10.1006/excr.1996.0115
Tomić-Canić M, Freedberg I, Blumenberg M. Codominant regulation of keratin gene expression by cell surface receptors and nuclear receptors. in Experimental Cell Research. 1996;224(1):96-102. doi:10.1006/excr.1996.0115 .
Tomić-Canić, Marjana, Freedberg, IM, Blumenberg, M, "Codominant regulation of keratin gene expression by cell surface receptors and nuclear receptors" in Experimental Cell Research, 224, no. 1 (1996):96-102, https://doi.org/10.1006/excr.1996.0115 . .