Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters
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2018
Authors
Todorović, LidijaStanojević, Boban
Mandušić, Vesna
Petrović, Nina
Živaljević, Vladan R.
Paunović, Ivan R.
Diklić, Aleksandar
Saenko, Vladimir
Yamashita, Shunichi
Article (Published version)
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A growing body of evidence suggests a role of the von Hippel-Lindau (VHL) tumor suppressor gene in the progression of papillary thyroid carcinoma (PTC). Our previous study of VHL in PTCs showed that lower VHL expression was associated with aggressive tumor features, but we found no evidence for VHL downregulation through common genetic or epigenetic modifications. Several studies pointed to a role of microRNA-92a (miR-92a) in the regulation of VHL expression in different cancers. In the present study, we examined the expression levels of VHL mRNA and miR-92a in 42 pairs of PTCs and matched non-tumor thyroid tissues by means of quantitative RT-PCR. We explored the correlation between them and their association with clinicopathological parameters. The results revealed that both VHL and miR-92a were either up-or downregulated in PTCs compared to corresponding non-tumor tissues. On univariate analysis, lower VHL levels were significantly associated with extrathyroid spread (P = 0.022) and ...capsular invasion (P = 0.032). Multivariate analysis confirmed the association of low VHL with extrathyroid spread (OR 0.246, 95% CI 0.069-0.872, P = 0.038). Higher miR-92a among PTC tissues associated with the presence of nodal metastases (univariate analysis: P = 0.012; multivariate: OR 4.703, 95% CI 1.109-19.938, P = 0.036). A negative correlation between VHL and miR-92a was observed in a subgroup of PTCs having vascular invasion (P = 0.033, r = -0.673). The data here reported demonstrate that the expression of both VHL and miR-92a is deregulated in PTC tissues and that in some PTCs they may have opposite roles. These roles, as well as their diagnostic and/or prognostic utility, remain to be clarified.
Keywords:
VHL / miR-92a / PTC / Thyroid cancer / Expression / Aggressive featuresSource:
Medical Oncology, 2018, 35, 2Funding / projects:
- Molecular determinants for tumor marker design (RS-173049)
DOI: 10.1007/s12032-017-1066-3
ISSN: 1357-0560; 1559-131X
PubMed: 29340905
WoS: 000423983300003
Scopus: 2-s2.0-85044380814
Institution/Community
VinčaTY - JOUR AU - Todorović, Lidija AU - Stanojević, Boban AU - Mandušić, Vesna AU - Petrović, Nina AU - Živaljević, Vladan R. AU - Paunović, Ivan R. AU - Diklić, Aleksandar AU - Saenko, Vladimir AU - Yamashita, Shunichi PY - 2018 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1944 AB - A growing body of evidence suggests a role of the von Hippel-Lindau (VHL) tumor suppressor gene in the progression of papillary thyroid carcinoma (PTC). Our previous study of VHL in PTCs showed that lower VHL expression was associated with aggressive tumor features, but we found no evidence for VHL downregulation through common genetic or epigenetic modifications. Several studies pointed to a role of microRNA-92a (miR-92a) in the regulation of VHL expression in different cancers. In the present study, we examined the expression levels of VHL mRNA and miR-92a in 42 pairs of PTCs and matched non-tumor thyroid tissues by means of quantitative RT-PCR. We explored the correlation between them and their association with clinicopathological parameters. The results revealed that both VHL and miR-92a were either up-or downregulated in PTCs compared to corresponding non-tumor tissues. On univariate analysis, lower VHL levels were significantly associated with extrathyroid spread (P = 0.022) and capsular invasion (P = 0.032). Multivariate analysis confirmed the association of low VHL with extrathyroid spread (OR 0.246, 95% CI 0.069-0.872, P = 0.038). Higher miR-92a among PTC tissues associated with the presence of nodal metastases (univariate analysis: P = 0.012; multivariate: OR 4.703, 95% CI 1.109-19.938, P = 0.036). A negative correlation between VHL and miR-92a was observed in a subgroup of PTCs having vascular invasion (P = 0.033, r = -0.673). The data here reported demonstrate that the expression of both VHL and miR-92a is deregulated in PTC tissues and that in some PTCs they may have opposite roles. These roles, as well as their diagnostic and/or prognostic utility, remain to be clarified. T2 - Medical Oncology T1 - Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters VL - 35 IS - 2 DO - 10.1007/s12032-017-1066-3 ER -
@article{ author = "Todorović, Lidija and Stanojević, Boban and Mandušić, Vesna and Petrović, Nina and Živaljević, Vladan R. and Paunović, Ivan R. and Diklić, Aleksandar and Saenko, Vladimir and Yamashita, Shunichi", year = "2018", abstract = "A growing body of evidence suggests a role of the von Hippel-Lindau (VHL) tumor suppressor gene in the progression of papillary thyroid carcinoma (PTC). Our previous study of VHL in PTCs showed that lower VHL expression was associated with aggressive tumor features, but we found no evidence for VHL downregulation through common genetic or epigenetic modifications. Several studies pointed to a role of microRNA-92a (miR-92a) in the regulation of VHL expression in different cancers. In the present study, we examined the expression levels of VHL mRNA and miR-92a in 42 pairs of PTCs and matched non-tumor thyroid tissues by means of quantitative RT-PCR. We explored the correlation between them and their association with clinicopathological parameters. The results revealed that both VHL and miR-92a were either up-or downregulated in PTCs compared to corresponding non-tumor tissues. On univariate analysis, lower VHL levels were significantly associated with extrathyroid spread (P = 0.022) and capsular invasion (P = 0.032). Multivariate analysis confirmed the association of low VHL with extrathyroid spread (OR 0.246, 95% CI 0.069-0.872, P = 0.038). Higher miR-92a among PTC tissues associated with the presence of nodal metastases (univariate analysis: P = 0.012; multivariate: OR 4.703, 95% CI 1.109-19.938, P = 0.036). A negative correlation between VHL and miR-92a was observed in a subgroup of PTCs having vascular invasion (P = 0.033, r = -0.673). The data here reported demonstrate that the expression of both VHL and miR-92a is deregulated in PTC tissues and that in some PTCs they may have opposite roles. These roles, as well as their diagnostic and/or prognostic utility, remain to be clarified.", journal = "Medical Oncology", title = "Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters", volume = "35", number = "2", doi = "10.1007/s12032-017-1066-3" }
Todorović, L., Stanojević, B., Mandušić, V., Petrović, N., Živaljević, V. R., Paunović, I. R., Diklić, A., Saenko, V.,& Yamashita, S.. (2018). Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters. in Medical Oncology, 35(2). https://doi.org/10.1007/s12032-017-1066-3
Todorović L, Stanojević B, Mandušić V, Petrović N, Živaljević VR, Paunović IR, Diklić A, Saenko V, Yamashita S. Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters. in Medical Oncology. 2018;35(2). doi:10.1007/s12032-017-1066-3 .
Todorović, Lidija, Stanojević, Boban, Mandušić, Vesna, Petrović, Nina, Živaljević, Vladan R., Paunović, Ivan R., Diklić, Aleksandar, Saenko, Vladimir, Yamashita, Shunichi, "Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters" in Medical Oncology, 35, no. 2 (2018), https://doi.org/10.1007/s12032-017-1066-3 . .