Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats
Аутори
Popović, Nataša M.Pajović, Snežana B.
Stojiljković, Vesna
Todorović, Ana
Pejić, Snežana
Pavlović, Ivan
Gavrilović, Ljubica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study examined the effects of chronic restraint stress (CRS: 2 hours x 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantified by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our findings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.
Кључне речи:
chronic restraint stress / copper-zinc superoxide dismutase / manganese superoxide dismutase / catalase / hippocampus / ratsИзвор:
Acta Veterinaria, Beograd, 2017, 67, 4, 540-550Финансирање / пројекти:
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
DOI: 10.1515/acve-2017-0043
ISSN: 0567-8315; 1820-7448
WoS: 000419653200007
Scopus: 2-s2.0-85041233135
Институција/група
VinčaTY - JOUR AU - Popović, Nataša M. AU - Pajović, Snežana B. AU - Stojiljković, Vesna AU - Todorović, Ana AU - Pejić, Snežana AU - Pavlović, Ivan AU - Gavrilović, Ljubica PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1908 AB - This study examined the effects of chronic restraint stress (CRS: 2 hours x 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantified by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our findings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats. T2 - Acta Veterinaria, Beograd T1 - Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats VL - 67 IS - 4 SP - 540 EP - 550 DO - 10.1515/acve-2017-0043 ER -
@article{ author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Todorović, Ana and Pejić, Snežana and Pavlović, Ivan and Gavrilović, Ljubica", year = "2017", abstract = "This study examined the effects of chronic restraint stress (CRS: 2 hours x 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantified by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our findings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.", journal = "Acta Veterinaria, Beograd", title = "Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats", volume = "67", number = "4", pages = "540-550", doi = "10.1515/acve-2017-0043" }
Popović, N. M., Pajović, S. B., Stojiljković, V., Todorović, A., Pejić, S., Pavlović, I.,& Gavrilović, L.. (2017). Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats. in Acta Veterinaria, Beograd, 67(4), 540-550. https://doi.org/10.1515/acve-2017-0043
Popović NM, Pajović SB, Stojiljković V, Todorović A, Pejić S, Pavlović I, Gavrilović L. Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats. in Acta Veterinaria, Beograd. 2017;67(4):540-550. doi:10.1515/acve-2017-0043 .
Popović, Nataša M., Pajović, Snežana B., Stojiljković, Vesna, Todorović, Ana, Pejić, Snežana, Pavlović, Ivan, Gavrilović, Ljubica, "Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats" in Acta Veterinaria, Beograd, 67, no. 4 (2017):540-550, https://doi.org/10.1515/acve-2017-0043 . .