Determination of Enalapril in Pharmaceuticals using Electrochemical Sensing with Amperometric Detection

Abstract

In this work a new electrochemical method will be presented for the determination of enalapril in pharmaceutical tablets using unmodified screen printed electrode (SPE). The studies were done using amperommetric detection. Enalapril provides well defined, ovalshape oxidation peak at +1.05 V (vs. Ag/AgCl, 3.0 M KCl) in Britton-Robinson buffer solution (BRBS) at pH 5.0. After optimization of the experimental conditions, the influence of most common interferent compounds was tested. Under optimized experimental conditions, the signals were linearly proportional to the concentration of enalapril in the range from 2.5 to 90 mu M with a detection limit of 0.9 mu M. Repeatability of ten replicate measurements of 5 mu M enalapril was estimated to be 1.5%. Proposed method was successfully applied for the determination of the total amount of enalapril content in pharmaceutical preparations. Nevertheless, proposed method could be beneficial for the quick quantifications of enalapril in drugs using unmodified SPE electrode without any further treatment.