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dc.creatorNikolic, Nadja
dc.creatorČarkić, Jelena
dc.creatorDimitrijevic, Ivana Ilic
dc.creatorEljabo, Najib
dc.creatorRadunovic, Milena
dc.creatorAnicic, Boban
dc.creatorTanić, Nasta
dc.creatorFalk, Markus
dc.creatorMilašin, Jelena
dc.date.accessioned2018-03-01T18:02:35Z
dc.date.available2018-03-01T18:02:35Z
dc.date.issued2018
dc.identifier.issn2212-4403 (print)
dc.identifier.issn1528-395X (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1862
dc.description.abstractObjective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P LT .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P LT .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175075/RS//
dc.rightsrestrictedAccessen
dc.sourceOral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
dc.titleP14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian populationen
dc.typearticleen
dcterms.abstractДимитријевиц, Ивана Илиц; Ељабо, Најиб; Радуновиц, Милена; Aнициц, Бобан; Таниц, Наста; Фалк, Маркус; Миласин, Јелена; Царкиц, Јелена; Николиц, Надја;
dc.citation.volume125
dc.citation.issue1
dc.citation.spage52
dc.citation.epage58
dc.identifier.wos000417487000010
dc.identifier.doi10.1016/j.oooo.2017.09.013
dc.identifier.pmid29079368
dc.identifier.scopus2-s2.0-85032179417


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