Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet
Само за регистроване кориснике
2017
Аутори
Jovanović, AleksandraObradović, Milan M.
Sudar-Milovanović, Emina
Stewart, Alan J.
Pitt, Samantha J.
Alavantić, Dragan
Aleksić, Ema
Isenović, Esma R.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150-200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the alpha(1) subunit of Na+/K+-ATPase by 30% (p LT 0.05), expression of total alpha(1) subunit of Na+/K+-ATPase by 31% (p LT 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p LT 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p LT 0.01) and 62% (p LT 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.
Кључне речи:
High-fat diet / Estradiol / Na+/K+-ATPase / Obesity / RhoA/ROCK signalling / FemaleИзвор:
Molecular and Cellular Biochemistry, 2017, 436, 1-2, 49-58Финансирање / пројекти:
- Хормонска регулација експресије и активности азот оксид синтазе и натријум-калијумове пумпе у експерименталним моделима инсулинске резистенције, дијабетеса и кардиоваскуларних поремећаја (RS-MESTD-Basic Research (BR or ON)-173033)
- Интегрална студија идентификације регионалних генетских фактора ризика и фактора ризика животне средине за масовне незаразне болести хумане популације у Србији - INGEMA_S (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41028)
DOI: 10.1007/s11010-017-3077-y
ISSN: 0300-8177; 1573-4919
PubMed: 28567564
WoS: 000414696600006
Scopus: 2-s2.0-85020098496
Колекције
Институција/група
VinčaTY - JOUR AU - Jovanović, Aleksandra AU - Obradović, Milan M. AU - Sudar-Milovanović, Emina AU - Stewart, Alan J. AU - Pitt, Samantha J. AU - Alavantić, Dragan AU - Aleksić, Ema AU - Isenović, Esma R. PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1812 AB - The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150-200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the alpha(1) subunit of Na+/K+-ATPase by 30% (p LT 0.05), expression of total alpha(1) subunit of Na+/K+-ATPase by 31% (p LT 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p LT 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p LT 0.01) and 62% (p LT 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats. T2 - Molecular and Cellular Biochemistry T1 - Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet VL - 436 IS - 1-2 SP - 49 EP - 58 DO - 10.1007/s11010-017-3077-y ER -
@article{ author = "Jovanović, Aleksandra and Obradović, Milan M. and Sudar-Milovanović, Emina and Stewart, Alan J. and Pitt, Samantha J. and Alavantić, Dragan and Aleksić, Ema and Isenović, Esma R.", year = "2017", abstract = "The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150-200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the alpha(1) subunit of Na+/K+-ATPase by 30% (p LT 0.05), expression of total alpha(1) subunit of Na+/K+-ATPase by 31% (p LT 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p LT 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p LT 0.01) and 62% (p LT 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.", journal = "Molecular and Cellular Biochemistry", title = "Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet", volume = "436", number = "1-2", pages = "49-58", doi = "10.1007/s11010-017-3077-y" }
Jovanović, A., Obradović, M. M., Sudar-Milovanović, E., Stewart, A. J., Pitt, S. J., Alavantić, D., Aleksić, E.,& Isenović, E. R.. (2017). Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet. in Molecular and Cellular Biochemistry, 436(1-2), 49-58. https://doi.org/10.1007/s11010-017-3077-y
Jovanović A, Obradović MM, Sudar-Milovanović E, Stewart AJ, Pitt SJ, Alavantić D, Aleksić E, Isenović ER. Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet. in Molecular and Cellular Biochemistry. 2017;436(1-2):49-58. doi:10.1007/s11010-017-3077-y .
Jovanović, Aleksandra, Obradović, Milan M., Sudar-Milovanović, Emina, Stewart, Alan J., Pitt, Samantha J., Alavantić, Dragan, Aleksić, Ema, Isenović, Esma R., "Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet" in Molecular and Cellular Biochemistry, 436, no. 1-2 (2017):49-58, https://doi.org/10.1007/s11010-017-3077-y . .