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dc.creatorStanković, Dalibor M.
dc.creatorŠvorc, Lubomir
dc.creatorMariano, Jose F. M. L.
dc.creatorOrtner, Astrid
dc.creatorKalcher, Kurt
dc.date.accessioned2018-03-01T17:54:41Z
dc.date.available2018-03-01T17:54:41Z
dc.date.issued2017
dc.identifier.issn1040-0397 (print)
dc.identifier.issn1521-4109 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1771
dc.description.abstractColchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19V, and second one at around 1.37V, in Britton-Robinson buffer solution at pH7.5. Wide dynamic range from 1 to 100 mu M was obtained with a detection limit (3 sigma(intercept)/slope) of a 0.26 mu M, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172030/RS//
dc.relationMagbiovin project [621375], Grant Agency of the Slovak Republic [1/0489/16]
dc.rightsrestrictedAccessen
dc.sourceElectroanalysisen
dc.subjectdifferential pulse voltammetryen
dc.subjectboron doped diamond electrodeen
dc.subjectnatural alkaloiden
dc.subjectDNAen
dc.subjectcolchicineen
dc.titleElectrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNAen
dc.typearticleen
dcterms.abstractСворц, Лубомир; Мариано, Јосе Ф. М. Л.; Ортнер, Aстрид; Калцхер, Курт; Станковић Далибор;
dc.citation.volume29
dc.citation.issue10
dc.citation.spage2276
dc.citation.epage2281
dc.identifier.wos000412547200014
dc.identifier.doi10.1002/elan.201700233
dc.identifier.scopus2-s2.0-85023162696


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