Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats
АуториRomić, Snježana Đ.
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Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in F...A handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.
Кључне речи:Obesity / Aging / Skeletal muscle / FAT/CD36 / Lipin-1
Извор:General Physiology and Biophysics, 2017, 36, 4, 399-406
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-41009)
- SAIA National Scholarship Programme of the Slovak Republic, [VEGA 2/0174/17], [APVV-15-0229], [APVV-15-0565]
ISSN: 0231-5882 (print); 1338-4325 (electronic)