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dc.creatorKeta, Otilija D.
dc.creatorBulat, Tanja M.
dc.creatorKorićanac, Lela
dc.creatorŽakula, Jelena
dc.creatorCuttone, Giacomo
dc.creatorPrivitera, Giuseppe
dc.creatorPetrović, Ivan M.
dc.creatorRistić-Fira, Aleksandra
dc.date.accessioned2018-03-01T15:29:54Z
dc.date.available2018-03-01T15:29:54Z
dc.date.issued2014
dc.identifier.issn1451-3994 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/167
dc.description.abstractMolecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173046/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/171019/RS//
dc.relationNational Laboratories of the South, National Institute for Nuclear Physics, Catania, Italy
dc.rightsopenAccessen
dc.sourceNuclear technology and radiation protectionen
dc.subjecthuman lung adenocarcinoma cellen
dc.subjectgamma-rayen
dc.subjectDNA damageen
dc.subjecterlotiniben
dc.subjectradiosensitizationen
dc.titleRadiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibitionen
dc.typearticleen
dcterms.abstractКорићанац Лела; Булат Тања М.; Петровић Иван; Кета, Отилија Д.; Привитера, Гиусеппе; Жакула Јелена; Ристић-Фира Aлександра; Цуттоне, Гиацомо;
dc.citation.volume29
dc.citation.issue3
dc.citation.spage233
dc.citation.epage241
dc.identifier.wos000343688300010
dc.identifier.doi10.2298/NTRP1403233K
dc.citation.rankM23
dc.identifier.scopus2-s2.0-84926672049
dc.identifier.fulltexthttp://vinar.vin.bg.ac.rs//bitstream/id/11993/163.pdf


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