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dc.creatorDojčilović, Radovan
dc.creatorPajović, Jelena D.
dc.creatorBožanić, Dušan K.
dc.creatorBogdanović, Una
dc.creatorVodnik, Vesna
dc.creatorDimitrijević-Branković, Suzana I.
dc.creatorMiljković, Miona G.
dc.creatorKascakova, Slavka
dc.creatorRefregiers, Matthieu
dc.creatorĐoković, Vladimir
dc.date.accessioned2018-03-01T17:40:24Z
dc.date.available2018-03-01T17:40:24Z
dc.date.issued2017
dc.identifier.issn0927-7765 (print)
dc.identifier.issn1873-4367 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1608
dc.description.abstractThe interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells surfaces. (C) 2017 Elsevier B.V. All rights reserved.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45020/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172056/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/171029/RS//
dc.relation[20131218]
dc.rightsrestrictedAccessen
dc.sourceColloids and Surfaces. B: Biointerfacesen
dc.subjectSilver nanoparticlesen
dc.subjectTryptophanen
dc.subjectFunctionalizationen
dc.subjectC. albicansen
dc.subjectFluorescence imagingen
dc.titleInteraction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging studyen
dc.typearticleen
dcterms.abstractКасцакова, Славка; Дојчиловић Радован; Пајовиц, Јелена Д.; Бозанић Душан; Богдановић Уна; Водник Весна; Димитријевиц-Бранковиц, Сузана; Миљковиц, Миона Г.; Рефрегиерс, Маттхиеу; Ђоковић Владимир;
dc.citation.volume155
dc.citation.spage341
dc.citation.epage348
dc.identifier.wos000403738000040
dc.identifier.doi10.1016/j.colsurfb.2017.04.044
dc.identifier.pmid28454063
dc.identifier.scopus2-s2.0-85018651384


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