The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains
Authors
Dimkić, IvicaStanković, Slavka
Nišavić, Marija
Petković, Marijana
Ristivojević, Petar
Fira, Đorđe
Berić, Tanja
Article (Published version)
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In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins). The ethyl acetate extracts produced the most favorable results. The extracts of SS-12.6, SS-13.1, and SS-38.4 showed the highest inhibition zones. An iturin analog is responsible for the inhibition of Xanthomonas arboricola and Pseudomonas syringae phytopathogenic strains. HPTLC bioautography effectively identified the active compounds from a mixture of lipopeptide extracts, proving in ...situ its potential for use in direct detection and determination of antimicrobials. In the test of potential synergism among individual extracts used in different mixtures, stronger antimicrobial effects were not observed. Biochemical and phylogenetic analysis clustered isolates SS-12.6, SS-13.1, SS-27.2, and SS-38.4 together with Bacillus amyloliquefaciens, while SS-10.7 was more closely related to Bacillus pumilus.
Keywords:
Bacillus / lipopeptides / MALDI-TOF MS / HPTLC / iturin / ethyl acetate extract / bioautographySource:
Frontiers in Microbiology, 2017, 8Funding / projects:
DOI: 10.3389/fmicb.2017.00925
ISSN: 1664-302X
PubMed: 28588570
WoS: 000402236600001
Scopus: 2-s2.0-85019725862
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VinčaTY - JOUR AU - Dimkić, Ivica AU - Stanković, Slavka AU - Nišavić, Marija AU - Petković, Marijana AU - Ristivojević, Petar AU - Fira, Đorđe AU - Berić, Tanja PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1591 AB - In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins). The ethyl acetate extracts produced the most favorable results. The extracts of SS-12.6, SS-13.1, and SS-38.4 showed the highest inhibition zones. An iturin analog is responsible for the inhibition of Xanthomonas arboricola and Pseudomonas syringae phytopathogenic strains. HPTLC bioautography effectively identified the active compounds from a mixture of lipopeptide extracts, proving in situ its potential for use in direct detection and determination of antimicrobials. In the test of potential synergism among individual extracts used in different mixtures, stronger antimicrobial effects were not observed. Biochemical and phylogenetic analysis clustered isolates SS-12.6, SS-13.1, SS-27.2, and SS-38.4 together with Bacillus amyloliquefaciens, while SS-10.7 was more closely related to Bacillus pumilus. T2 - Frontiers in Microbiology T1 - The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains VL - 8 DO - 10.3389/fmicb.2017.00925 ER -
@article{ author = "Dimkić, Ivica and Stanković, Slavka and Nišavić, Marija and Petković, Marijana and Ristivojević, Petar and Fira, Đorđe and Berić, Tanja", year = "2017", abstract = "In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins). The ethyl acetate extracts produced the most favorable results. The extracts of SS-12.6, SS-13.1, and SS-38.4 showed the highest inhibition zones. An iturin analog is responsible for the inhibition of Xanthomonas arboricola and Pseudomonas syringae phytopathogenic strains. HPTLC bioautography effectively identified the active compounds from a mixture of lipopeptide extracts, proving in situ its potential for use in direct detection and determination of antimicrobials. In the test of potential synergism among individual extracts used in different mixtures, stronger antimicrobial effects were not observed. Biochemical and phylogenetic analysis clustered isolates SS-12.6, SS-13.1, SS-27.2, and SS-38.4 together with Bacillus amyloliquefaciens, while SS-10.7 was more closely related to Bacillus pumilus.", journal = "Frontiers in Microbiology", title = "The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains", volume = "8", doi = "10.3389/fmicb.2017.00925" }
Dimkić, I., Stanković, S., Nišavić, M., Petković, M., Ristivojević, P., Fira, Đ.,& Berić, T.. (2017). The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. in Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.00925
Dimkić I, Stanković S, Nišavić M, Petković M, Ristivojević P, Fira Đ, Berić T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. in Frontiers in Microbiology. 2017;8. doi:10.3389/fmicb.2017.00925 .
Dimkić, Ivica, Stanković, Slavka, Nišavić, Marija, Petković, Marijana, Ristivojević, Petar, Fira, Đorđe, Berić, Tanja, "The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains" in Frontiers in Microbiology, 8 (2017), https://doi.org/10.3389/fmicb.2017.00925 . .