Wiring of glucose oxidase with graphene nanoribbons: an electrochemical third generation glucose biosensor
Само за регистроване кориснике
2017
Аутори
Mehmeti, EdaStanković, Dalibor M.
Chaiyo, Sudkate
Zavasnik, Janez
Zagar, Kristina
Kalcher, Kurt
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A reagentless third generation electrochemical glucose biosensor was fabricated based on wiring the template enzyme glucose oxidase (GOx) with graphene nanoribbons (GN) in order to create direct electron transfer between the co-factor (flavin adenine dinucleotide, FAD) and the electrode. The strategy involved: (i) isolation of the apo-enzyme by separating it from its co-enzyme; (ii) preparation of graphene nanoribbons (GN) by oxidative unzipping of multi-walled carbon nanotubes; (iii) adsorptive immobilization of GNs on the surface of a screen printed carbon electrode (SPCE); (iv) covalent attachment of FAD to the nanoribbons; (v) recombination of the apo-enzyme with the covalently bound FAD to the holoenzyme; and (vi) stabilization of the bio-layer with a thin membrane of Nafion. The biosensor (referred to as GN/FAD/apo-GOx/Nafion/SPCE) is operated at a potential of +0.475 V vs Ag/AgCl/{3 M KCl} in flow-injection mode with an oxygen-free phosphate buffer (pH 7.5) acting as a carrier. ...The signals are linearly proportional to the concentration of glucose in the range from 50 to 2000 mgai...L-1 with a detection limit of 20 mgai...L-1. The repeatability (10 measurements, at 1000 mgai...L-1 glucose) is +/- 1.4% and the reproducibility (5 sensors, 1000 mgai...L-1 glucose) is +/- 1.8%. The biosensor was applied to the determination of glucose in human serum.
Кључне речи:
Enzyme wiring / Screen printed carbon electrode / Flow injection analysis / Apo-glucose oxidase / Glucose oxidase / Enzyme biosensor / FAD / Nafion / Multiwalled carbon nanotubes / MWCNT / AmperometryИзвор:
Microchimica Acta, 2017, 184, 4, 1127-1134Финансирање / пројекти:
- Higher KOS Stipendien from ADA, MEST, Austrian Agency for International Cooperation in Education and Research (OeAD-GmbH)
- Centre for International Cooperation Mobility (ICM)
- Asea UniNet
DOI: 10.1007/s00604-017-2115-5
ISSN: 0026-3672; 1436-5073
WoS: 000397497900018
Scopus: 2-s2.0-85011874591
Колекције
Институција/група
VinčaTY - JOUR AU - Mehmeti, Eda AU - Stanković, Dalibor M. AU - Chaiyo, Sudkate AU - Zavasnik, Janez AU - Zagar, Kristina AU - Kalcher, Kurt PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1477 AB - A reagentless third generation electrochemical glucose biosensor was fabricated based on wiring the template enzyme glucose oxidase (GOx) with graphene nanoribbons (GN) in order to create direct electron transfer between the co-factor (flavin adenine dinucleotide, FAD) and the electrode. The strategy involved: (i) isolation of the apo-enzyme by separating it from its co-enzyme; (ii) preparation of graphene nanoribbons (GN) by oxidative unzipping of multi-walled carbon nanotubes; (iii) adsorptive immobilization of GNs on the surface of a screen printed carbon electrode (SPCE); (iv) covalent attachment of FAD to the nanoribbons; (v) recombination of the apo-enzyme with the covalently bound FAD to the holoenzyme; and (vi) stabilization of the bio-layer with a thin membrane of Nafion. The biosensor (referred to as GN/FAD/apo-GOx/Nafion/SPCE) is operated at a potential of +0.475 V vs Ag/AgCl/{3 M KCl} in flow-injection mode with an oxygen-free phosphate buffer (pH 7.5) acting as a carrier. The signals are linearly proportional to the concentration of glucose in the range from 50 to 2000 mgai...L-1 with a detection limit of 20 mgai...L-1. The repeatability (10 measurements, at 1000 mgai...L-1 glucose) is +/- 1.4% and the reproducibility (5 sensors, 1000 mgai...L-1 glucose) is +/- 1.8%. The biosensor was applied to the determination of glucose in human serum. T2 - Microchimica Acta T1 - Wiring of glucose oxidase with graphene nanoribbons: an electrochemical third generation glucose biosensor VL - 184 IS - 4 SP - 1127 EP - 1134 DO - 10.1007/s00604-017-2115-5 ER -
@article{ author = "Mehmeti, Eda and Stanković, Dalibor M. and Chaiyo, Sudkate and Zavasnik, Janez and Zagar, Kristina and Kalcher, Kurt", year = "2017", abstract = "A reagentless third generation electrochemical glucose biosensor was fabricated based on wiring the template enzyme glucose oxidase (GOx) with graphene nanoribbons (GN) in order to create direct electron transfer between the co-factor (flavin adenine dinucleotide, FAD) and the electrode. The strategy involved: (i) isolation of the apo-enzyme by separating it from its co-enzyme; (ii) preparation of graphene nanoribbons (GN) by oxidative unzipping of multi-walled carbon nanotubes; (iii) adsorptive immobilization of GNs on the surface of a screen printed carbon electrode (SPCE); (iv) covalent attachment of FAD to the nanoribbons; (v) recombination of the apo-enzyme with the covalently bound FAD to the holoenzyme; and (vi) stabilization of the bio-layer with a thin membrane of Nafion. The biosensor (referred to as GN/FAD/apo-GOx/Nafion/SPCE) is operated at a potential of +0.475 V vs Ag/AgCl/{3 M KCl} in flow-injection mode with an oxygen-free phosphate buffer (pH 7.5) acting as a carrier. The signals are linearly proportional to the concentration of glucose in the range from 50 to 2000 mgai...L-1 with a detection limit of 20 mgai...L-1. The repeatability (10 measurements, at 1000 mgai...L-1 glucose) is +/- 1.4% and the reproducibility (5 sensors, 1000 mgai...L-1 glucose) is +/- 1.8%. The biosensor was applied to the determination of glucose in human serum.", journal = "Microchimica Acta", title = "Wiring of glucose oxidase with graphene nanoribbons: an electrochemical third generation glucose biosensor", volume = "184", number = "4", pages = "1127-1134", doi = "10.1007/s00604-017-2115-5" }
Mehmeti, E., Stanković, D. M., Chaiyo, S., Zavasnik, J., Zagar, K.,& Kalcher, K.. (2017). Wiring of glucose oxidase with graphene nanoribbons: an electrochemical third generation glucose biosensor. in Microchimica Acta, 184(4), 1127-1134. https://doi.org/10.1007/s00604-017-2115-5
Mehmeti E, Stanković DM, Chaiyo S, Zavasnik J, Zagar K, Kalcher K. Wiring of glucose oxidase with graphene nanoribbons: an electrochemical third generation glucose biosensor. in Microchimica Acta. 2017;184(4):1127-1134. doi:10.1007/s00604-017-2115-5 .
Mehmeti, Eda, Stanković, Dalibor M., Chaiyo, Sudkate, Zavasnik, Janez, Zagar, Kristina, Kalcher, Kurt, "Wiring of glucose oxidase with graphene nanoribbons: an electrochemical third generation glucose biosensor" in Microchimica Acta, 184, no. 4 (2017):1127-1134, https://doi.org/10.1007/s00604-017-2115-5 . .