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dc.creatorManojlović, Dragica
dc.creatorDramićanin, Miroslav
dc.creatorMiletic, Vesna
dc.creatorMitić-Ćulafić, Dragana
dc.creatorJovanovic, Bojana
dc.creatorNikolić, Biljana
dc.date.accessioned2018-03-01T17:27:45Z
dc.date.available2018-03-01T17:27:45Z
dc.date.issued2017
dc.identifier.issn0109-5641 (print)
dc.identifier.issn1879-0097 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1462
dc.description.abstractObjective. To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects. Methods. Cytotoxic and genotoxic effects of BisGMA, FIT, TEGDMA, CQ DMAEMA and TPO and their combined toxicity in four clinically relevant mixtures (FIT/TPO, FIT/CQ BisGMA/TPO, BisGMA/CQ) were tested on human fetal lung fibroblasts MRC-5 using MTT and Comet assays. We assessed combination effects of monomers and photoinitiators on overall toxicity from the measured concentration-effect relationships. Combination index (CI) was calculated on the basis of the median-effect equation derived from the mass-action law principle. Results. Individual substances showed decreasing cytotoxic effects in the following order: BisGMA GT TPO GT FIT GT CQ GT DMAEMA GT TEGDMA. Experimental mixtures showed decreasing cytotoxic effects in the order BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. FIT-based mixtures exhibited antagonistic cytotoxic effects between components while BisGMA-based mixtures demonstrated synergistic effects at ED50 TPO amplified both antagonistic and synergistic cytotoxic effects in mixtures. Pure substances showed genotoxicity in the following order: TPO GT BisGMA GT FIT GT CQ GT TEGDMA. We did not detect the genotoxic potential of DMAEMA. The rank of genotoxic concentrations of the mixtures was: BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. Significance. Lower cytotoxicity and genotoxicity of FIT than BisGMA suggests its greater bio-co mpatibility. Conversely, photoinitiator TPO was significantly more cytotoxic and genotoxic than both CQ and DMAEMA. CI values showed that components of FIT-based mixtures exhibit an antagonistic cytotoxic effect, while compontents of BisGMA-based mixtures show synergism. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45020/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172007/RS//
dc.rightsrestrictedAccessen
dc.sourceDental Materialsen
dc.subjectCytotoxicityen
dc.subjectGenotoxicityen
dc.subjectCombinatorial effecten
dc.subjectMonomeren
dc.subjectPhotoinitiatoren
dc.titleCytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixturesen
dc.typearticleen
dcterms.abstractМилетиц, Весна; Драмићанин Мирослав; Јовановиц, Бојана; Николиц, Биљана; Митиц-Цулафиц, Драгана; Манојловиц, Драгица;
dc.citation.volume33
dc.citation.issue4
dc.citation.spage454
dc.citation.epage466
dc.identifier.wos000396410200014
dc.identifier.doi10.1016/j.dental.2017.02.002
dc.identifier.pmid28258769
dc.identifier.scopus2-s2.0-85014043733


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