Efficient two-frequency ultrasound extraction of beta-carotene from the fungus Blakeslea trispora
АуториAvdeenko, Anatoly P.
Belova, Elena A.
Dasic, Predrag V.
Konovalova, Svetlana A.
Baklanova, Larisa V.
Krstić, Sanja S.
Milosavljevic, Milutin M.
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Carotenoids are known for their lower environmental pollution, lower costs, shorter extraction time compared to regular extraction methods and higher extraction efficiency. Ultrasound has significant advantages in the extraction of a great number of biologically active compounds including carotenoids. Benefits of using ultrasound for extraction of various compounds are numerous and include effective mixing and micro-mixing, more efficient energy transfer, reduced thermal and concentration gradients, reduced temperature. The aim of this work was to investigate the influence of two-frequency ultrasound main parameters such as ultrasonic intensity, sonication time, and temperature. Application of two-frequency ultrasound (simultaneous influence of ultrasound with frequency of 20-46 kHz, intensity of 1.5-2.5 W/cm(2) and frequency of 1.0-2.0 MHz, intensity of 2.0- 3.0 W/cm(2)) raises the percentage of beta-carotene extraction from biomass of the fungus Blakeslea trispora from 90-92% up to 9...8-99% in comparison with one frequency ultrasound. The recommended oil temperature for the extraction process is 35 degrees C, because lower temperature leads to reduction of percentage of beta-carotene extraction, and higher temperature promotes unjustified increase of expenditure of energy.
Кључне речи:ultrasound / beta-carotene / Blakeslea trispora / two-frequency ultrasound
Извор:Hemijska industrija, 2017, 71, 4, 329-336
ISSN: 0367-598X (print); 2217-7426 (electronic)