Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres
Roux, Brianna M.
Bajić, Vladan P.
Brey, Eric M.
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Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is ...needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.
Keywords:biomaterials / genotoxicity / HUVECs / MSCs / tissue engineering
Source:Journal of Biomedical Materials Research. Part A, 2017, 105, 1, 284-291
- Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-173034)
- National Science Foundation [CBET-1263994], Veterans Administration [5 I01 BX000418-06], Fulbright Program