Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases
Аутори
Čolović, Mirjana B.Ma, Tian
Ma, Xiang
Isaković, Anđelka
Misirlić-Denčić, Sonja
Kortz, Urlich
Krstić, Danijela
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polyoxopalladates (POPs) are discrete, anionic palladium(II)- oxo nanoclusters combining properties of polyoxometalates and palladium(II), and thus are highly promising for the development of novel antitumor metallodrugs. The aim of this study was to investigate in vitro the influence of three POP salts with approved anti-neuroblastoma action, Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), and Na6[Pd13O8(PhAsO3)8]·23H2O (Pd13L), on E-NTPDase activity using rat synaptic plasma membranes (SPMs) isolated from Wistar brain as a model system. Dose-dependent inhibition of ENTPDases was obtained within concentration range 2 × 10-6 - 1 × 10-3 mol/L for all investigated POPs. Inhibition parameters, IC50 value and Hill's coefficient, nH, were determined by sigmoidal fitting the experimental results. The calculated IC50 values were (1.08 ± 0.25) × 10-4 , (1.19 ± 0.13) × 10-4 , and (2.06 ± 0.88) × 10-4 mol/L for Pd13, SrPd12, and Pd13L, respectively, in...dicating their similar inhibitory strengths. The nH values were determined to be < 1, indicating negatively cooperative binding for all POPs studied. The observed inhibitory effect of these anti-neuroblastoma POPs on ENTPDase activity suggest that the inhibition of E-NTPDases, the enzymes representing the major part of purinergic signaling, could be considered as a putative mechanism of antitumor action and a new strategy in the development of novel antitumor therapeutics.
Извор:
PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts, 2021, 77-77Издавач:
- Belgrade : Society of Physical Chemists of Serbia
Напомена:
- The book of abstracts available at: http://www.socphyschemserb.org/media/physical-chemistry-2021/abstracts.pdf
Колекције
Институција/група
VinčaTY - CONF AU - Čolović, Mirjana B. AU - Ma, Tian AU - Ma, Xiang AU - Isaković, Anđelka AU - Misirlić-Denčić, Sonja AU - Kortz, Urlich AU - Krstić, Danijela PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/13018 AB - Polyoxopalladates (POPs) are discrete, anionic palladium(II)- oxo nanoclusters combining properties of polyoxometalates and palladium(II), and thus are highly promising for the development of novel antitumor metallodrugs. The aim of this study was to investigate in vitro the influence of three POP salts with approved anti-neuroblastoma action, Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), and Na6[Pd13O8(PhAsO3)8]·23H2O (Pd13L), on E-NTPDase activity using rat synaptic plasma membranes (SPMs) isolated from Wistar brain as a model system. Dose-dependent inhibition of ENTPDases was obtained within concentration range 2 × 10-6 - 1 × 10-3 mol/L for all investigated POPs. Inhibition parameters, IC50 value and Hill's coefficient, nH, were determined by sigmoidal fitting the experimental results. The calculated IC50 values were (1.08 ± 0.25) × 10-4 , (1.19 ± 0.13) × 10-4 , and (2.06 ± 0.88) × 10-4 mol/L for Pd13, SrPd12, and Pd13L, respectively, indicating their similar inhibitory strengths. The nH values were determined to be < 1, indicating negatively cooperative binding for all POPs studied. The observed inhibitory effect of these anti-neuroblastoma POPs on ENTPDase activity suggest that the inhibition of E-NTPDases, the enzymes representing the major part of purinergic signaling, could be considered as a putative mechanism of antitumor action and a new strategy in the development of novel antitumor therapeutics. PB - Belgrade : Society of Physical Chemists of Serbia C3 - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts T1 - Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases SP - 77 EP - 77 UR - https://hdl.handle.net/21.15107/rcub_vinar_13018 ER -
@conference{ author = "Čolović, Mirjana B. and Ma, Tian and Ma, Xiang and Isaković, Anđelka and Misirlić-Denčić, Sonja and Kortz, Urlich and Krstić, Danijela", year = "2021", abstract = "Polyoxopalladates (POPs) are discrete, anionic palladium(II)- oxo nanoclusters combining properties of polyoxometalates and palladium(II), and thus are highly promising for the development of novel antitumor metallodrugs. The aim of this study was to investigate in vitro the influence of three POP salts with approved anti-neuroblastoma action, Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), and Na6[Pd13O8(PhAsO3)8]·23H2O (Pd13L), on E-NTPDase activity using rat synaptic plasma membranes (SPMs) isolated from Wistar brain as a model system. Dose-dependent inhibition of ENTPDases was obtained within concentration range 2 × 10-6 - 1 × 10-3 mol/L for all investigated POPs. Inhibition parameters, IC50 value and Hill's coefficient, nH, were determined by sigmoidal fitting the experimental results. The calculated IC50 values were (1.08 ± 0.25) × 10-4 , (1.19 ± 0.13) × 10-4 , and (2.06 ± 0.88) × 10-4 mol/L for Pd13, SrPd12, and Pd13L, respectively, indicating their similar inhibitory strengths. The nH values were determined to be < 1, indicating negatively cooperative binding for all POPs studied. The observed inhibitory effect of these anti-neuroblastoma POPs on ENTPDase activity suggest that the inhibition of E-NTPDases, the enzymes representing the major part of purinergic signaling, could be considered as a putative mechanism of antitumor action and a new strategy in the development of novel antitumor therapeutics.", publisher = "Belgrade : Society of Physical Chemists of Serbia", journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts", title = "Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases", pages = "77-77", url = "https://hdl.handle.net/21.15107/rcub_vinar_13018" }
Čolović, M. B., Ma, T., Ma, X., Isaković, A., Misirlić-Denčić, S., Kortz, U.,& Krstić, D.. (2021). Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts Belgrade : Society of Physical Chemists of Serbia., 77-77. https://hdl.handle.net/21.15107/rcub_vinar_13018
Čolović MB, Ma T, Ma X, Isaković A, Misirlić-Denčić S, Kortz U, Krstić D. Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts. 2021;:77-77. https://hdl.handle.net/21.15107/rcub_vinar_13018 .
Čolović, Mirjana B., Ma, Tian, Ma, Xiang, Isaković, Anđelka, Misirlić-Denčić, Sonja, Kortz, Urlich, Krstić, Danijela, "Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts (2021):77-77, https://hdl.handle.net/21.15107/rcub_vinar_13018 .