Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa
Genotoxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent
Само за регистроване кориснике
2023
Аутори
Čolović, MirjanaGajski, Goran
Gerić, M.
Domijan, Ana-Marija
Savić, N.
Parac-Vogt, Tatjana
Krstić, Danijela
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
UVOD: Polioksovolframati su negativno naelektrisana neorganska kompleksna jedinjenja koja se dve
decenije intenzivno proučavaju kao moćni bioaktivni agensi. Skorašnje studije na dugim kostima i
bubrezima miša su pokazale da poliokosvoframati mogu da se koriste kao agensi za bojenje za poboljšanje
kontrasta (CESAs) za kompjuterizovanu tomografiju (CT).
CILJ: S obzirom da razvoj novih medicinskih agenasa zahteva procenu njihove bezbednosti, cilj ove
studije je da se evaluiraju genotoksične osobine in vitro polioksovolframata Wells-Dawson tipa (parent
WD), α2-K6P2W18O62.14H2O, koji je u prethodnim istraživanjima pokazao bolje osobine u odnosu na
joheksol, standardni kontrastni agens za CT.
METODOLOGIJA: Parent WD je sintetisan sledeći ovjavljenu proceduru. Uzorci humane krvi dobijeni od
zdravih donora su tretirani ispitivanim polioksovolframatom, a zatim inkubirani na 37 °C tokom 4 i 24
sata. Za procenu genotoksičnosti parent WD korišćen je alkalni komet test kako je opisano u l...iteraturi.
REZULTATI: Uzorci pune krvi su tretirani parent WD polioksovolframatom u opsegu koncentracija
1-100 μmol/L. Oštećenje DNK, koje se koristi kao pokazatelj genotoksičnosti, je izraženo kao % repne
DNK. Dobijeni rezultati su pokazali da sve ispitivane koncentracije parent WD nisu značajno uticale na
oštećenje DNK u odnosu na odgovarajuću kontrolu, posle 4 i 24 sata tretmana.
ZAKLJUČAK: Parent WD nanoklaster nije izazvao genotoksični efekat na zdravim ćelijama periferne
humane krvi u svim ispitivanim koncentracijama. U skladu sa tim, ispitivani potencijalni CESA kandidat
za CT bi se u daljim istraživanjima mogao smatrati bezbednim sa stanovišta genotoksičnosti.
INTRODUCTION: Polyoxotungstates are negatively charged inorganic cage complexes that were studied
as potent bioactive agents for the last two decades. They were recently reported as promising
contrast-enhancing staining agents (CESAs) for computed tomography (CT) of murine long bones and
kidney tissues.
AIM: Taking into account the fact that the development of novel medications requires an evaluation
of their safety, the purpose of this study was to assess the genotoxic properties in vitro of parent
Wells-Dawson polyoxotungstate (parent WD), α2-K6P2W18O62.14H2O that showed superior imaging
capabilities compared to the standard CT contrast agent iohexol.
METHODOLOGY: Parent WD was synthesized according to the published procedure. Human whole
blood samples obtained from healthy donors were treated with parent WD, and then incubated at 37
°C for 4 and 24 h. For the genotoxicity assessment of the studied nanocluster alkaline comet assay was
performed as described in the literatu...re.
RESULTS: Whole blood samples were treated with parent WD within the concentration range of 1-100
μmol/L. DNA damage was expressed as % of tail DNA and used as the indicator of genotoxicity. The
obtained results demonstrated that parent WD at all investigated concentrations did not significantly
affect DNA damage with respect to the corresponding control, for both 4 and 24 h.
CONCLUSION: Parent WD nanocluster did not induce a genotoxic effect on normal non-target human
peripheral blood cells at all studied concentrations. Thus, this promising CESA candidate for CT could
be considered in further research as safe from a genotoxicity point of view.
Кључне речи:
Wells-Dawson polioksovolframat / kontrastni agens / DNK oštećenje / genotoksičnost in vitro / Wells-Dawson polyoxotungstate / contrast agent / DNA damage / genotoxicity in vitroИзвор:
13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book, 2023, 191-192Издавач:
- Belgrade : Serbian Society of Toxicology
Напомена:
- 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : May 10-12, 2023, Belgrade.
Колекције
Институција/група
VinčaTY - CONF AU - Čolović, Mirjana AU - Gajski, Goran AU - Gerić, M. AU - Domijan, Ana-Marija AU - Savić, N. AU - Parac-Vogt, Tatjana AU - Krstić, Danijela PY - 2023 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12930 AB - UVOD: Polioksovolframati su negativno naelektrisana neorganska kompleksna jedinjenja koja se dve decenije intenzivno proučavaju kao moćni bioaktivni agensi. Skorašnje studije na dugim kostima i bubrezima miša su pokazale da poliokosvoframati mogu da se koriste kao agensi za bojenje za poboljšanje kontrasta (CESAs) za kompjuterizovanu tomografiju (CT). CILJ: S obzirom da razvoj novih medicinskih agenasa zahteva procenu njihove bezbednosti, cilj ove studije je da se evaluiraju genotoksične osobine in vitro polioksovolframata Wells-Dawson tipa (parent WD), α2-K6P2W18O62.14H2O, koji je u prethodnim istraživanjima pokazao bolje osobine u odnosu na joheksol, standardni kontrastni agens za CT. METODOLOGIJA: Parent WD je sintetisan sledeći ovjavljenu proceduru. Uzorci humane krvi dobijeni od zdravih donora su tretirani ispitivanim polioksovolframatom, a zatim inkubirani na 37 °C tokom 4 i 24 sata. Za procenu genotoksičnosti parent WD korišćen je alkalni komet test kako je opisano u literaturi. REZULTATI: Uzorci pune krvi su tretirani parent WD polioksovolframatom u opsegu koncentracija 1-100 μmol/L. Oštećenje DNK, koje se koristi kao pokazatelj genotoksičnosti, je izraženo kao % repne DNK. Dobijeni rezultati su pokazali da sve ispitivane koncentracije parent WD nisu značajno uticale na oštećenje DNK u odnosu na odgovarajuću kontrolu, posle 4 i 24 sata tretmana. ZAKLJUČAK: Parent WD nanoklaster nije izazvao genotoksični efekat na zdravim ćelijama periferne humane krvi u svim ispitivanim koncentracijama. U skladu sa tim, ispitivani potencijalni CESA kandidat za CT bi se u daljim istraživanjima mogao smatrati bezbednim sa stanovišta genotoksičnosti. AB - INTRODUCTION: Polyoxotungstates are negatively charged inorganic cage complexes that were studied as potent bioactive agents for the last two decades. They were recently reported as promising contrast-enhancing staining agents (CESAs) for computed tomography (CT) of murine long bones and kidney tissues. AIM: Taking into account the fact that the development of novel medications requires an evaluation of their safety, the purpose of this study was to assess the genotoxic properties in vitro of parent Wells-Dawson polyoxotungstate (parent WD), α2-K6P2W18O62.14H2O that showed superior imaging capabilities compared to the standard CT contrast agent iohexol. METHODOLOGY: Parent WD was synthesized according to the published procedure. Human whole blood samples obtained from healthy donors were treated with parent WD, and then incubated at 37 °C for 4 and 24 h. For the genotoxicity assessment of the studied nanocluster alkaline comet assay was performed as described in the literature. RESULTS: Whole blood samples were treated with parent WD within the concentration range of 1-100 μmol/L. DNA damage was expressed as % of tail DNA and used as the indicator of genotoxicity. The obtained results demonstrated that parent WD at all investigated concentrations did not significantly affect DNA damage with respect to the corresponding control, for both 4 and 24 h. CONCLUSION: Parent WD nanocluster did not induce a genotoxic effect on normal non-target human peripheral blood cells at all studied concentrations. Thus, this promising CESA candidate for CT could be considered in further research as safe from a genotoxicity point of view. PB - Belgrade : Serbian Society of Toxicology C3 - 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book T1 - Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa T1 - Genotoxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent SP - 191 EP - 192 UR - https://hdl.handle.net/21.15107/rcub_vinar_12930 ER -
@conference{ author = "Čolović, Mirjana and Gajski, Goran and Gerić, M. and Domijan, Ana-Marija and Savić, N. and Parac-Vogt, Tatjana and Krstić, Danijela", year = "2023", abstract = "UVOD: Polioksovolframati su negativno naelektrisana neorganska kompleksna jedinjenja koja se dve decenije intenzivno proučavaju kao moćni bioaktivni agensi. Skorašnje studije na dugim kostima i bubrezima miša su pokazale da poliokosvoframati mogu da se koriste kao agensi za bojenje za poboljšanje kontrasta (CESAs) za kompjuterizovanu tomografiju (CT). CILJ: S obzirom da razvoj novih medicinskih agenasa zahteva procenu njihove bezbednosti, cilj ove studije je da se evaluiraju genotoksične osobine in vitro polioksovolframata Wells-Dawson tipa (parent WD), α2-K6P2W18O62.14H2O, koji je u prethodnim istraživanjima pokazao bolje osobine u odnosu na joheksol, standardni kontrastni agens za CT. METODOLOGIJA: Parent WD je sintetisan sledeći ovjavljenu proceduru. Uzorci humane krvi dobijeni od zdravih donora su tretirani ispitivanim polioksovolframatom, a zatim inkubirani na 37 °C tokom 4 i 24 sata. Za procenu genotoksičnosti parent WD korišćen je alkalni komet test kako je opisano u literaturi. REZULTATI: Uzorci pune krvi su tretirani parent WD polioksovolframatom u opsegu koncentracija 1-100 μmol/L. Oštećenje DNK, koje se koristi kao pokazatelj genotoksičnosti, je izraženo kao % repne DNK. Dobijeni rezultati su pokazali da sve ispitivane koncentracije parent WD nisu značajno uticale na oštećenje DNK u odnosu na odgovarajuću kontrolu, posle 4 i 24 sata tretmana. ZAKLJUČAK: Parent WD nanoklaster nije izazvao genotoksični efekat na zdravim ćelijama periferne humane krvi u svim ispitivanim koncentracijama. U skladu sa tim, ispitivani potencijalni CESA kandidat za CT bi se u daljim istraživanjima mogao smatrati bezbednim sa stanovišta genotoksičnosti., INTRODUCTION: Polyoxotungstates are negatively charged inorganic cage complexes that were studied as potent bioactive agents for the last two decades. They were recently reported as promising contrast-enhancing staining agents (CESAs) for computed tomography (CT) of murine long bones and kidney tissues. AIM: Taking into account the fact that the development of novel medications requires an evaluation of their safety, the purpose of this study was to assess the genotoxic properties in vitro of parent Wells-Dawson polyoxotungstate (parent WD), α2-K6P2W18O62.14H2O that showed superior imaging capabilities compared to the standard CT contrast agent iohexol. METHODOLOGY: Parent WD was synthesized according to the published procedure. Human whole blood samples obtained from healthy donors were treated with parent WD, and then incubated at 37 °C for 4 and 24 h. For the genotoxicity assessment of the studied nanocluster alkaline comet assay was performed as described in the literature. RESULTS: Whole blood samples were treated with parent WD within the concentration range of 1-100 μmol/L. DNA damage was expressed as % of tail DNA and used as the indicator of genotoxicity. The obtained results demonstrated that parent WD at all investigated concentrations did not significantly affect DNA damage with respect to the corresponding control, for both 4 and 24 h. CONCLUSION: Parent WD nanocluster did not induce a genotoxic effect on normal non-target human peripheral blood cells at all studied concentrations. Thus, this promising CESA candidate for CT could be considered in further research as safe from a genotoxicity point of view.", publisher = "Belgrade : Serbian Society of Toxicology", journal = "13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book", title = "Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa, Genotoxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent", pages = "191-192", url = "https://hdl.handle.net/21.15107/rcub_vinar_12930" }
Čolović, M., Gajski, G., Gerić, M., Domijan, A., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2023). Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa. in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book Belgrade : Serbian Society of Toxicology., 191-192. https://hdl.handle.net/21.15107/rcub_vinar_12930
Čolović M, Gajski G, Gerić M, Domijan A, Savić N, Parac-Vogt T, Krstić D. Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa. in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book. 2023;:191-192. https://hdl.handle.net/21.15107/rcub_vinar_12930 .
Čolović, Mirjana, Gajski, Goran, Gerić, M., Domijan, Ana-Marija, Savić, N., Parac-Vogt, Tatjana, Krstić, Danijela, "Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa" in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book (2023):191-192, https://hdl.handle.net/21.15107/rcub_vinar_12930 .