Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro
Hafnium(IV)-substituted Wells-Dawson based contrast-enhancing staining agent: effect on DNA in vitro
Само за регистроване кориснике
2023
Аутори
Čolović, MirjanaGajski, Goran
Gerić, M.
Domijan, Ana-Marija
Savić, N.
Parac-Vogt, Tatjana
Krstić, Danijela
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
UVOD: Istraživanja pokazuju da su metalne nanočestice pogodni kandidati za kontrastna sredstva
(CESA-contrast-enhancing staining agents) nove generacije za kompjuterizovanu tomografiju (CT)
zahvaljujući velikoj gustini i sposobnosti da apsorbuju X-zrake.
CILJ: Studije toksičnosti in vivo ukazuju na štetno dejstvo polioksometalata, što bi mogla biti glavna
prepreka u kliničkoj primeni ovih bioaktivnih kompleksnih jedinjenja. Stoga je cilj ovog istraživanja da
se ispitaju genotoksične osobine polioksovolframata Wells-Dawson tipa supstituisanih sa hafnijumom
(Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O, koji je pokazao dobre osobine kao kontrastno sredstvo za
vizuelizaciju tkiva bubrega i dugih kostiju miša.
METODOLOGIJA: Hf-WD 1:2 je sintetisan prema metodi opisanoj u literaturi. Uzorci humane pune krvi
su uzeti od zdravih donora i izloženi različitim koncentracijama (10-6-10-4 mol/L) Hf-WD 1:2 tokom 4
i 24 sata, na 37 °C. Standardna metoda za alkalni komet test je korišćena za pra...ćenje genotoksičnih
efekata Hf-WD 1:2.
REZULTATI: Stepen oštećenja DNK humane periferne krvi nakon izlaganja Hf-WD 1:2 je izražen kao relativno
povećanje repne DNK u odnosu na kontrolu. Nije uočena statistički značajna razlika u količini prekida
DNK lanca u poređenju sa odgovarajućom kontrolom, za oba vremena izlaganja (4 i 24 sata) i za sve
ispitivane koncentracije polioksovolframata.
ZAKLJUČAK: In vitro ispitivanje genotoksičnosti pokazalo je da Hf-WD 1:2 ne menja značajno strukturu
DNK zdravih humanih ćelija pune krvi i stoga se može smatrati bezbednim u smislu genotoksičnosti u
dodatnim studijama kao potencijalnog kontrastnog sredstva za CT.
INTRODUCTION: Metallic nanoparticles have been reported as promising candidates for the development
of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT)
due to their high X-ray attenuation and density.
AIM: In vivo toxicity studies revealed side effects of polyoxometalate clusters, which could be a key
obstacle in the potential clinical application of these bioactive compounds. Therefore, the aim of this
investigation was to perform the genotoxicity evaluation of 1:2 hafnium(IV)-substituted Wells-Dawson
polyoxotungstate (Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O that was reported as a promising CESA
candidate to visualize murine long bones and kidneys.
METHODOLOGY: Hf-WD 1:2 was prepared using the method described in the literature. Human whole
blood samples were taken from healthy donors and exposed to different Hf-WD 1:2 concentrations
(within the range of 10-6-10-4 mol/L) for 4 and 24 h, at 37 °C. The standard procedure for alkaline comet...
assay was carried out for monitoring the genotoxic effects of Hf-WD 1:2.
RESULTS: The degree of DNA damage in human peripheral blood cells after exposure to Hf-WD 1:2 was
expressed as a relative increase of tail DNA related to the control. No statistically significant difference
in the amount of DNA strand breaks in comparison with the corresponding control was observed for
both 4 and 24 h, regardless of the Hf-WD 1:2 concentrations tested.
CONCLUSION: In vitro genotoxicity assessment indicated that Hf-WD 1:2 does not affect DNA structure
in healthy human blood cells, accordingly might be regarded as genotoxicity safe in further research as
a potential CESA for CT.
Кључне речи:
hafnijum(IV)-supstituisani Wells-Dawson polioksovolframat / kontrastno sredstvo / alkalni komet esej / genotoksičnost in vitro / hafnium(IV)-substituted Wells-Dawson polyoxotungstate / contrast-enhancing staining agent / alkaline comet assay / genotoxicity in vitroИзвор:
13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book, 2023, 189-190Издавач:
- Belgrade : Serbian Society of Toxicology
Напомена:
- 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : May 10-12, 2023, Belgrade.
Колекције
Институција/група
VinčaTY - CONF AU - Čolović, Mirjana AU - Gajski, Goran AU - Gerić, M. AU - Domijan, Ana-Marija AU - Savić, N. AU - Parac-Vogt, Tatjana AU - Krstić, Danijela PY - 2023 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12929 AB - UVOD: Istraživanja pokazuju da su metalne nanočestice pogodni kandidati za kontrastna sredstva (CESA-contrast-enhancing staining agents) nove generacije za kompjuterizovanu tomografiju (CT) zahvaljujući velikoj gustini i sposobnosti da apsorbuju X-zrake. CILJ: Studije toksičnosti in vivo ukazuju na štetno dejstvo polioksometalata, što bi mogla biti glavna prepreka u kliničkoj primeni ovih bioaktivnih kompleksnih jedinjenja. Stoga je cilj ovog istraživanja da se ispitaju genotoksične osobine polioksovolframata Wells-Dawson tipa supstituisanih sa hafnijumom (Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O, koji je pokazao dobre osobine kao kontrastno sredstvo za vizuelizaciju tkiva bubrega i dugih kostiju miša. METODOLOGIJA: Hf-WD 1:2 je sintetisan prema metodi opisanoj u literaturi. Uzorci humane pune krvi su uzeti od zdravih donora i izloženi različitim koncentracijama (10-6-10-4 mol/L) Hf-WD 1:2 tokom 4 i 24 sata, na 37 °C. Standardna metoda za alkalni komet test je korišćena za praćenje genotoksičnih efekata Hf-WD 1:2. REZULTATI: Stepen oštećenja DNK humane periferne krvi nakon izlaganja Hf-WD 1:2 je izražen kao relativno povećanje repne DNK u odnosu na kontrolu. Nije uočena statistički značajna razlika u količini prekida DNK lanca u poređenju sa odgovarajućom kontrolom, za oba vremena izlaganja (4 i 24 sata) i za sve ispitivane koncentracije polioksovolframata. ZAKLJUČAK: In vitro ispitivanje genotoksičnosti pokazalo je da Hf-WD 1:2 ne menja značajno strukturu DNK zdravih humanih ćelija pune krvi i stoga se može smatrati bezbednim u smislu genotoksičnosti u dodatnim studijama kao potencijalnog kontrastnog sredstva za CT. AB - INTRODUCTION: Metallic nanoparticles have been reported as promising candidates for the development of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT) due to their high X-ray attenuation and density. AIM: In vivo toxicity studies revealed side effects of polyoxometalate clusters, which could be a key obstacle in the potential clinical application of these bioactive compounds. Therefore, the aim of this investigation was to perform the genotoxicity evaluation of 1:2 hafnium(IV)-substituted Wells-Dawson polyoxotungstate (Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O that was reported as a promising CESA candidate to visualize murine long bones and kidneys. METHODOLOGY: Hf-WD 1:2 was prepared using the method described in the literature. Human whole blood samples were taken from healthy donors and exposed to different Hf-WD 1:2 concentrations (within the range of 10-6-10-4 mol/L) for 4 and 24 h, at 37 °C. The standard procedure for alkaline comet assay was carried out for monitoring the genotoxic effects of Hf-WD 1:2. RESULTS: The degree of DNA damage in human peripheral blood cells after exposure to Hf-WD 1:2 was expressed as a relative increase of tail DNA related to the control. No statistically significant difference in the amount of DNA strand breaks in comparison with the corresponding control was observed for both 4 and 24 h, regardless of the Hf-WD 1:2 concentrations tested. CONCLUSION: In vitro genotoxicity assessment indicated that Hf-WD 1:2 does not affect DNA structure in healthy human blood cells, accordingly might be regarded as genotoxicity safe in further research as a potential CESA for CT. PB - Belgrade : Serbian Society of Toxicology C3 - 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book T1 - Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro T1 - Hafnium(IV)-substituted Wells-Dawson based contrast-enhancing staining agent: effect on DNA in vitro SP - 189 EP - 190 UR - https://hdl.handle.net/21.15107/rcub_vinar_12929 ER -
@conference{ author = "Čolović, Mirjana and Gajski, Goran and Gerić, M. and Domijan, Ana-Marija and Savić, N. and Parac-Vogt, Tatjana and Krstić, Danijela", year = "2023", abstract = "UVOD: Istraživanja pokazuju da su metalne nanočestice pogodni kandidati za kontrastna sredstva (CESA-contrast-enhancing staining agents) nove generacije za kompjuterizovanu tomografiju (CT) zahvaljujući velikoj gustini i sposobnosti da apsorbuju X-zrake. CILJ: Studije toksičnosti in vivo ukazuju na štetno dejstvo polioksometalata, što bi mogla biti glavna prepreka u kliničkoj primeni ovih bioaktivnih kompleksnih jedinjenja. Stoga je cilj ovog istraživanja da se ispitaju genotoksične osobine polioksovolframata Wells-Dawson tipa supstituisanih sa hafnijumom (Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O, koji je pokazao dobre osobine kao kontrastno sredstvo za vizuelizaciju tkiva bubrega i dugih kostiju miša. METODOLOGIJA: Hf-WD 1:2 je sintetisan prema metodi opisanoj u literaturi. Uzorci humane pune krvi su uzeti od zdravih donora i izloženi različitim koncentracijama (10-6-10-4 mol/L) Hf-WD 1:2 tokom 4 i 24 sata, na 37 °C. Standardna metoda za alkalni komet test je korišćena za praćenje genotoksičnih efekata Hf-WD 1:2. REZULTATI: Stepen oštećenja DNK humane periferne krvi nakon izlaganja Hf-WD 1:2 je izražen kao relativno povećanje repne DNK u odnosu na kontrolu. Nije uočena statistički značajna razlika u količini prekida DNK lanca u poređenju sa odgovarajućom kontrolom, za oba vremena izlaganja (4 i 24 sata) i za sve ispitivane koncentracije polioksovolframata. ZAKLJUČAK: In vitro ispitivanje genotoksičnosti pokazalo je da Hf-WD 1:2 ne menja značajno strukturu DNK zdravih humanih ćelija pune krvi i stoga se može smatrati bezbednim u smislu genotoksičnosti u dodatnim studijama kao potencijalnog kontrastnog sredstva za CT., INTRODUCTION: Metallic nanoparticles have been reported as promising candidates for the development of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT) due to their high X-ray attenuation and density. AIM: In vivo toxicity studies revealed side effects of polyoxometalate clusters, which could be a key obstacle in the potential clinical application of these bioactive compounds. Therefore, the aim of this investigation was to perform the genotoxicity evaluation of 1:2 hafnium(IV)-substituted Wells-Dawson polyoxotungstate (Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O that was reported as a promising CESA candidate to visualize murine long bones and kidneys. METHODOLOGY: Hf-WD 1:2 was prepared using the method described in the literature. Human whole blood samples were taken from healthy donors and exposed to different Hf-WD 1:2 concentrations (within the range of 10-6-10-4 mol/L) for 4 and 24 h, at 37 °C. The standard procedure for alkaline comet assay was carried out for monitoring the genotoxic effects of Hf-WD 1:2. RESULTS: The degree of DNA damage in human peripheral blood cells after exposure to Hf-WD 1:2 was expressed as a relative increase of tail DNA related to the control. No statistically significant difference in the amount of DNA strand breaks in comparison with the corresponding control was observed for both 4 and 24 h, regardless of the Hf-WD 1:2 concentrations tested. CONCLUSION: In vitro genotoxicity assessment indicated that Hf-WD 1:2 does not affect DNA structure in healthy human blood cells, accordingly might be regarded as genotoxicity safe in further research as a potential CESA for CT.", publisher = "Belgrade : Serbian Society of Toxicology", journal = "13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book", title = "Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro, Hafnium(IV)-substituted Wells-Dawson based contrast-enhancing staining agent: effect on DNA in vitro", pages = "189-190", url = "https://hdl.handle.net/21.15107/rcub_vinar_12929" }
Čolović, M., Gajski, G., Gerić, M., Domijan, A., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2023). Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro. in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book Belgrade : Serbian Society of Toxicology., 189-190. https://hdl.handle.net/21.15107/rcub_vinar_12929
Čolović M, Gajski G, Gerić M, Domijan A, Savić N, Parac-Vogt T, Krstić D. Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro. in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book. 2023;:189-190. https://hdl.handle.net/21.15107/rcub_vinar_12929 .
Čolović, Mirjana, Gajski, Goran, Gerić, M., Domijan, Ana-Marija, Savić, N., Parac-Vogt, Tatjana, Krstić, Danijela, "Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro" in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book (2023):189-190, https://hdl.handle.net/21.15107/rcub_vinar_12929 .