Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography
Само за регистроване кориснике
2022
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cytotoxicity assessment in vitro of three Wells-Dawson polyoxometalates (POMs) as contrast-enhancing staining agent (CESA) candidates: parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), was carried out on healthy non-target human blood cells. Whole blood samples were treated with these POMs (1-100 μM) for 4 and 24 h at 37 °C. The results showed a time-dependent decrease of the peripheral blood mononuclear cell (PBMC) viability for all investigated polyoxotungstates. The 4 h treatment induced a significant cytotoxic effect only for the higher concentrations of Hf-WD 1:2, whereas 24 h exposure resulted in a concentration-dependent alteration of the cell viability. The most potent cytotoxic activity was obtained for Hf-WD 1:2 inducing a decrease of the cell viability from 25 to 45% compared to the corresponding control. The weake...st effect was observed for parent WD, which resulted in no statistically significant difference in PBMC viability in respect to the control at the concentration of 1 μM. Finally, parent WD, lacunary WD, and Hf-WD 1:2 could not be considered safe from a cytotoxic point of view, thus further more extensive toxicity studies are needed.
Извор:
PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings, 2022, 1, 275-278Издавач:
- Belgrade : Society of Physical Chemists of Serbia
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
- POMCACT - In Vitro and in Vivo Evaluation of Novel Polyoxometalate Based Contrast Agents for Micro Computed Tomography (RS-ScienceFundRS-Dijaspora-6526393)
- Bilateral project Serbia-Croatia [No. 337-00-205/2019-09/19]
- Erasmus+ KA103 European Mobility Program
Напомена:
- 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : September 26-30, Belgrade.
Колекције
Институција/група
VinčaTY - CONF AU - Čolović, Mirjana AU - Gajski, Goran AU - Gerić, M. AU - Savić, Nada AU - Krstić, Danijela PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12924 AB - Cytotoxicity assessment in vitro of three Wells-Dawson polyoxometalates (POMs) as contrast-enhancing staining agent (CESA) candidates: parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), was carried out on healthy non-target human blood cells. Whole blood samples were treated with these POMs (1-100 μM) for 4 and 24 h at 37 °C. The results showed a time-dependent decrease of the peripheral blood mononuclear cell (PBMC) viability for all investigated polyoxotungstates. The 4 h treatment induced a significant cytotoxic effect only for the higher concentrations of Hf-WD 1:2, whereas 24 h exposure resulted in a concentration-dependent alteration of the cell viability. The most potent cytotoxic activity was obtained for Hf-WD 1:2 inducing a decrease of the cell viability from 25 to 45% compared to the corresponding control. The weakest effect was observed for parent WD, which resulted in no statistically significant difference in PBMC viability in respect to the control at the concentration of 1 μM. Finally, parent WD, lacunary WD, and Hf-WD 1:2 could not be considered safe from a cytotoxic point of view, thus further more extensive toxicity studies are needed. PB - Belgrade : Society of Physical Chemists of Serbia C3 - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings T1 - Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography VL - 1 SP - 275 EP - 278 UR - https://hdl.handle.net/21.15107/rcub_vinar_12924 ER -
@conference{ author = "Čolović, Mirjana and Gajski, Goran and Gerić, M. and Savić, Nada and Krstić, Danijela", year = "2022", abstract = "Cytotoxicity assessment in vitro of three Wells-Dawson polyoxometalates (POMs) as contrast-enhancing staining agent (CESA) candidates: parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), was carried out on healthy non-target human blood cells. Whole blood samples were treated with these POMs (1-100 μM) for 4 and 24 h at 37 °C. The results showed a time-dependent decrease of the peripheral blood mononuclear cell (PBMC) viability for all investigated polyoxotungstates. The 4 h treatment induced a significant cytotoxic effect only for the higher concentrations of Hf-WD 1:2, whereas 24 h exposure resulted in a concentration-dependent alteration of the cell viability. The most potent cytotoxic activity was obtained for Hf-WD 1:2 inducing a decrease of the cell viability from 25 to 45% compared to the corresponding control. The weakest effect was observed for parent WD, which resulted in no statistically significant difference in PBMC viability in respect to the control at the concentration of 1 μM. Finally, parent WD, lacunary WD, and Hf-WD 1:2 could not be considered safe from a cytotoxic point of view, thus further more extensive toxicity studies are needed.", publisher = "Belgrade : Society of Physical Chemists of Serbia", journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings", title = "Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography", volume = "1", pages = "275-278", url = "https://hdl.handle.net/21.15107/rcub_vinar_12924" }
Čolović, M., Gajski, G., Gerić, M., Savić, N.,& Krstić, D.. (2022). Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings Belgrade : Society of Physical Chemists of Serbia., 1, 275-278. https://hdl.handle.net/21.15107/rcub_vinar_12924
Čolović M, Gajski G, Gerić M, Savić N, Krstić D. Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings. 2022;1:275-278. https://hdl.handle.net/21.15107/rcub_vinar_12924 .
Čolović, Mirjana, Gajski, Goran, Gerić, M., Savić, Nada, Krstić, Danijela, "Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings, 1 (2022):275-278, https://hdl.handle.net/21.15107/rcub_vinar_12924 .