Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu
Effects of melatonin treatment on catecholamine synthesis, degradation and α1-and β2-adrenergic receptors in prefrontal cortex of rats exposed to the chronic mild unpredictable stress
Само за регистроване кориснике
2019
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Disrupted circadian rhythm and sleep disorders are one of the crucial symptoms of major depressive disorder. While physiological role of melatonin and its antioxidative properties are well known, increasing number of studies show that melatonin may also exhibit antidepressive effects. Aim of our research was to examine effects of melatonin on catecholamine turnover and receptors in prefrontal cortex (PFC) of rats exposed to the chronic mild unpredictable stress (CUMS) model of depression. Two months old Wistar rats were subjected to the 4 weeks of CUMS. Both control animals and animals exposed to the CUMS were treated with either melatonin (10 mg/kg, i.p.) or vehicle. Levels of tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), catechol-O-methyltransferase (COMT), α1- and β2-adrenergic receptors in PFC were examined using Western blot procedure. Catecholamine synthesis through TH and DBH in PFC was not affected either by CUMS procedure or by melatonin treatment. However, anima...ls exposed to the CUMS shoved elevated levels of COMT and decreased levels of α1- and β2-adrenergic receptors in PFC. Melatonin treatment improved levels of adrenergic receptors and decreased levels of COMT protein to the levels comparable with control animals. These findings show that melatonin treatment had protective role on PFC in chronic stress. Our results suggest that some of the melatonin effects were achieved through regulation of COMT and α1- and β2-adrenergic receptors protein levels. These preliminary results support potential therapeutic use of melatonin in treatment of depressive disorders.
Извор:
14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova, 2019, 206-207Издавач:
- Beograd : Srpsko farmakološko društvo (SFD)
Напомена:
- 14th Serbian Congress of Pharmacology and 4th Serbian Congress of Clinical Pharmacology : September 18-21, 2019, Novi Sad.
Колекције
Институција/група
VinčaTY - CONF AU - Ferizović, Harisa AU - Spasojević, Nataša AU - Stefanović, Bojana AU - Dronjak, Slađana PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12813 AB - Disrupted circadian rhythm and sleep disorders are one of the crucial symptoms of major depressive disorder. While physiological role of melatonin and its antioxidative properties are well known, increasing number of studies show that melatonin may also exhibit antidepressive effects. Aim of our research was to examine effects of melatonin on catecholamine turnover and receptors in prefrontal cortex (PFC) of rats exposed to the chronic mild unpredictable stress (CUMS) model of depression. Two months old Wistar rats were subjected to the 4 weeks of CUMS. Both control animals and animals exposed to the CUMS were treated with either melatonin (10 mg/kg, i.p.) or vehicle. Levels of tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), catechol-O-methyltransferase (COMT), α1- and β2-adrenergic receptors in PFC were examined using Western blot procedure. Catecholamine synthesis through TH and DBH in PFC was not affected either by CUMS procedure or by melatonin treatment. However, animals exposed to the CUMS shoved elevated levels of COMT and decreased levels of α1- and β2-adrenergic receptors in PFC. Melatonin treatment improved levels of adrenergic receptors and decreased levels of COMT protein to the levels comparable with control animals. These findings show that melatonin treatment had protective role on PFC in chronic stress. Our results suggest that some of the melatonin effects were achieved through regulation of COMT and α1- and β2-adrenergic receptors protein levels. These preliminary results support potential therapeutic use of melatonin in treatment of depressive disorders. PB - Beograd : Srpsko farmakološko društvo (SFD) C3 - 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova T1 - Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu T1 - Effects of melatonin treatment on catecholamine synthesis, degradation and α1-and β2-adrenergic receptors in prefrontal cortex of rats exposed to the chronic mild unpredictable stress SP - 206 EP - 207 UR - https://hdl.handle.net/21.15107/rcub_vinar_12813 ER -
@conference{ author = "Ferizović, Harisa and Spasojević, Nataša and Stefanović, Bojana and Dronjak, Slađana", year = "2019", abstract = "Disrupted circadian rhythm and sleep disorders are one of the crucial symptoms of major depressive disorder. While physiological role of melatonin and its antioxidative properties are well known, increasing number of studies show that melatonin may also exhibit antidepressive effects. Aim of our research was to examine effects of melatonin on catecholamine turnover and receptors in prefrontal cortex (PFC) of rats exposed to the chronic mild unpredictable stress (CUMS) model of depression. Two months old Wistar rats were subjected to the 4 weeks of CUMS. Both control animals and animals exposed to the CUMS were treated with either melatonin (10 mg/kg, i.p.) or vehicle. Levels of tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), catechol-O-methyltransferase (COMT), α1- and β2-adrenergic receptors in PFC were examined using Western blot procedure. Catecholamine synthesis through TH and DBH in PFC was not affected either by CUMS procedure or by melatonin treatment. However, animals exposed to the CUMS shoved elevated levels of COMT and decreased levels of α1- and β2-adrenergic receptors in PFC. Melatonin treatment improved levels of adrenergic receptors and decreased levels of COMT protein to the levels comparable with control animals. These findings show that melatonin treatment had protective role on PFC in chronic stress. Our results suggest that some of the melatonin effects were achieved through regulation of COMT and α1- and β2-adrenergic receptors protein levels. These preliminary results support potential therapeutic use of melatonin in treatment of depressive disorders.", publisher = "Beograd : Srpsko farmakološko društvo (SFD)", journal = "14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova", title = "Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu, Effects of melatonin treatment on catecholamine synthesis, degradation and α1-and β2-adrenergic receptors in prefrontal cortex of rats exposed to the chronic mild unpredictable stress", pages = "206-207", url = "https://hdl.handle.net/21.15107/rcub_vinar_12813" }
Ferizović, H., Spasojević, N., Stefanović, B.,& Dronjak, S.. (2019). Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu. in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova Beograd : Srpsko farmakološko društvo (SFD)., 206-207. https://hdl.handle.net/21.15107/rcub_vinar_12813
Ferizović H, Spasojević N, Stefanović B, Dronjak S. Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu. in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova. 2019;:206-207. https://hdl.handle.net/21.15107/rcub_vinar_12813 .
Ferizović, Harisa, Spasojević, Nataša, Stefanović, Bojana, Dronjak, Slađana, "Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu" in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova (2019):206-207, https://hdl.handle.net/21.15107/rcub_vinar_12813 .