Effects of Synthesis Parameters on Structure and Antimicrobial Properties of Bacterial Cellulose/Hydroxyapatite/TiO2 Polymer–Ceramic Composite Material
Аутори
Sknepnek, AleksandraFilipović, Suzana
Pavlović, Vladimir B.
Mirković, Nemanja
Miletić, Dunja
Gržetić, Jelena
Mirković, Miljana M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Bacterial cellulose (BC) is a highly pure polysaccharide biopolymer that can be produced by various bacterial genera. Even though BC lacks functional properties, its porosity, three-dimensional network, and high specific surface area make it a suitable carrier for functional composite materials. In the present study, BC-producing bacteria were isolated from kombucha beverage and identified using a molecular method. Two sets of the BC hydrogels were produced in static conditions after four and seven days. Afterwards, two different synthesis pathways were applied for BC functionalization. The first method implied the incorporation of previously synthesized HAp/TiO2 nanocomposite using an immersion technique, while the second method included the functionalization of BC during the synthesis of HAp/TiO2 nanocomposite in the reaction mixture. The primary goal was to find the best method to obtain the functionalized material. Physicochemical and microstructural properties were analyzed by SEM..., EDS, FTIR, and XRD methods. Further properties were examined by tensile test and thermogravimetric analysis, and antimicrobial activity was assessed by a total plate count assay. The results showed that HAp/TiO2 was successfully incorporated into the produced BC hydrogels using both methods. The applied methods of incorporation influenced the differences in morphology, phase distribution, mechanical and thermal properties, and antimicrobial activity against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Proteus mirabilis (ATCC 12453), and Candida albicans (ATCC 10231). Composite material can be recommended for further development and application in environments that are suitable for diseases spreading.
Кључне речи:
bacterial cellulose / Komagataetibacter rhaeticus / hydroxyapatite / titanium dioxide / polymer–ceramic material / antimicrobial activityИзвор:
Polymers, 2024, 16, 4, 470-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200175 (Институт техничких наука САНУ, Београд) (RS-MESTD-inst-2020-200175)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200325 (Војнотехнички институт - ВТИ, Београд) (RS-MESTD-inst-2020-200325)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
- project “Green technologies for obtaining antimicrobial composites for use in cosmetics”, “EU for Green Agenda in Serbia”, with the technical and financial support of the European Union and in partnership with the Ministry of Environmental Protection, implemented by UNDP in cooperation with the Embassy of Sweden and the European Investment Bank (EIB), with additional funding from the Governments of Sweden, Switzerland, and Serbia (Contract number 00136377/00127312/2023/24)
Колекције
Институција/група
VinčaTY - JOUR AU - Sknepnek, Aleksandra AU - Filipović, Suzana AU - Pavlović, Vladimir B. AU - Mirković, Nemanja AU - Miletić, Dunja AU - Gržetić, Jelena AU - Mirković, Miljana M. PY - 2024 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12793 AB - Bacterial cellulose (BC) is a highly pure polysaccharide biopolymer that can be produced by various bacterial genera. Even though BC lacks functional properties, its porosity, three-dimensional network, and high specific surface area make it a suitable carrier for functional composite materials. In the present study, BC-producing bacteria were isolated from kombucha beverage and identified using a molecular method. Two sets of the BC hydrogels were produced in static conditions after four and seven days. Afterwards, two different synthesis pathways were applied for BC functionalization. The first method implied the incorporation of previously synthesized HAp/TiO2 nanocomposite using an immersion technique, while the second method included the functionalization of BC during the synthesis of HAp/TiO2 nanocomposite in the reaction mixture. The primary goal was to find the best method to obtain the functionalized material. Physicochemical and microstructural properties were analyzed by SEM, EDS, FTIR, and XRD methods. Further properties were examined by tensile test and thermogravimetric analysis, and antimicrobial activity was assessed by a total plate count assay. The results showed that HAp/TiO2 was successfully incorporated into the produced BC hydrogels using both methods. The applied methods of incorporation influenced the differences in morphology, phase distribution, mechanical and thermal properties, and antimicrobial activity against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Proteus mirabilis (ATCC 12453), and Candida albicans (ATCC 10231). Composite material can be recommended for further development and application in environments that are suitable for diseases spreading. T2 - Polymers T1 - Effects of Synthesis Parameters on Structure and Antimicrobial Properties of Bacterial Cellulose/Hydroxyapatite/TiO2 Polymer–Ceramic Composite Material VL - 16 IS - 4 SP - 470 DO - 10.3390/polym16040470 ER -
@article{ author = "Sknepnek, Aleksandra and Filipović, Suzana and Pavlović, Vladimir B. and Mirković, Nemanja and Miletić, Dunja and Gržetić, Jelena and Mirković, Miljana M.", year = "2024", abstract = "Bacterial cellulose (BC) is a highly pure polysaccharide biopolymer that can be produced by various bacterial genera. Even though BC lacks functional properties, its porosity, three-dimensional network, and high specific surface area make it a suitable carrier for functional composite materials. In the present study, BC-producing bacteria were isolated from kombucha beverage and identified using a molecular method. Two sets of the BC hydrogels were produced in static conditions after four and seven days. Afterwards, two different synthesis pathways were applied for BC functionalization. The first method implied the incorporation of previously synthesized HAp/TiO2 nanocomposite using an immersion technique, while the second method included the functionalization of BC during the synthesis of HAp/TiO2 nanocomposite in the reaction mixture. The primary goal was to find the best method to obtain the functionalized material. Physicochemical and microstructural properties were analyzed by SEM, EDS, FTIR, and XRD methods. Further properties were examined by tensile test and thermogravimetric analysis, and antimicrobial activity was assessed by a total plate count assay. The results showed that HAp/TiO2 was successfully incorporated into the produced BC hydrogels using both methods. The applied methods of incorporation influenced the differences in morphology, phase distribution, mechanical and thermal properties, and antimicrobial activity against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Proteus mirabilis (ATCC 12453), and Candida albicans (ATCC 10231). Composite material can be recommended for further development and application in environments that are suitable for diseases spreading.", journal = "Polymers", title = "Effects of Synthesis Parameters on Structure and Antimicrobial Properties of Bacterial Cellulose/Hydroxyapatite/TiO2 Polymer–Ceramic Composite Material", volume = "16", number = "4", pages = "470", doi = "10.3390/polym16040470" }
Sknepnek, A., Filipović, S., Pavlović, V. B., Mirković, N., Miletić, D., Gržetić, J.,& Mirković, M. M.. (2024). Effects of Synthesis Parameters on Structure and Antimicrobial Properties of Bacterial Cellulose/Hydroxyapatite/TiO2 Polymer–Ceramic Composite Material. in Polymers, 16(4), 470. https://doi.org/10.3390/polym16040470
Sknepnek A, Filipović S, Pavlović VB, Mirković N, Miletić D, Gržetić J, Mirković MM. Effects of Synthesis Parameters on Structure and Antimicrobial Properties of Bacterial Cellulose/Hydroxyapatite/TiO2 Polymer–Ceramic Composite Material. in Polymers. 2024;16(4):470. doi:10.3390/polym16040470 .
Sknepnek, Aleksandra, Filipović, Suzana, Pavlović, Vladimir B., Mirković, Nemanja, Miletić, Dunja, Gržetić, Jelena, Mirković, Miljana M., "Effects of Synthesis Parameters on Structure and Antimicrobial Properties of Bacterial Cellulose/Hydroxyapatite/TiO2 Polymer–Ceramic Composite Material" in Polymers, 16, no. 4 (2024):470, https://doi.org/10.3390/polym16040470 . .