Show simple item record

dc.creatorJoksimovic, Nenad
dc.creatorBaskic, Dejan
dc.creatorPopovic, Suzana
dc.creatorZaric, Milan
dc.creatorKosanic, Marijana
dc.creatorRankovic, Branislav
dc.creatorStanojković, Tatjana P.
dc.creatorNovaković, Slađana B.
dc.creatorDavidovic, Goran
dc.creatorBugarcic, Zorica
dc.creatorJankovic, Nenad
dc.date.accessioned2018-03-01T17:10:48Z
dc.date.available2018-03-01T17:10:48Z
dc.date.issued2016
dc.identifier.issn1477-9226 (print)
dc.identifier.issn1477-9234 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1269
dc.description.abstractA serie of novel square pyramidal copper(II) complexes [Cu(L)(2)H2O] (3a-d) with O,O-bidentate ligands [L = ethyl-2-hydroxy-4-aryl-4-oxo-2-butenoate; aryl = 3-methoxyphenyl-2a, (E)-2-phenylvinyl-2b, (E)-2-(4-hydroxy-3-methoxyphenyl)vinyl-2c, 3-nitrophenyl-2d, 2-thienyl-2e] were synthesized and characterized by spectral (UV-Vis, IR, ESI-MS and EPR), elemental and X-ray analysis. The antimicrobial activity was estimated by the determination of the minimal inhibitory concentration (MIC) using the broth micro-dilution method. The most active antibacterial compounds were 3c and 3d, while the best antifungal activity was showed by complexes 3b and 3e. The lowest MIC value (0.048 mg mL(-1)) was measured for 3c against Proteus mirabilis. The cytotoxic activity was tested using the MTT method on human epithelial carcinoma HeLa cells, human lung carcinoma A549 cells and human colon carcinoma LS174 cells. All complexes showed extremely better cytotoxic activity compared to cisplatin at all tested concentrations. Compound 3d expressed the best activity against all tested cell lines with IC50 values ranging from 7.45 to 7.91 mu g mL(-1). The type of cell death and the impact on the cell cycle for 3d and 3e were evaluated by flow cytometry. Both compounds induced apoptosis and S phase cell cycle arrest. The interactions between selected complexes (3d and 3e) and CT-DNA or bovine serum albumin (BSA) were investigated by the fluorescence spectroscopic method. Competitive experiments with ethidium bromide (EB) indicated that 3d and 3e have a propensity to displace EB from the EB-DNA complex through intercalation suggesting strong competition with EB [K-sv = (1.4 +/- 0.2) and (2.9 +/- 0.1) x 10(4) M-1, respectively]. K-sv values indicate that these complexes bind to DNA covalently and non-covalently. The achieved results in the fluorescence titration of BSA with 3d and 3e [K-a = (2.9 +/- 0.2) x 10(6) and (2.5 +/- 0.2) x 10(5) M, respectively] showed that the fluorescence quenching of BSA is a result of the formation of the 3d- and 3e-BSA complexes. The obtained K-a values are high enough to ensure that a significant amount of 3d and 3e gets transported and distributed through the cells.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172011/RS//
dc.rightsrestrictedAccessen
dc.sourceDalton Transactionsen
dc.titleSynthesis, characterization, biological activity, DNA and BSA binding study: novel copper(II) complexes with 2-hydroxy-4-aryl-4-oxo-2-butenoateen
dc.typearticleen
dcterms.abstractБаскиц, Дејан; Зариц, Милан; Поповиц, Сузана; Новаковић Слађана; Станојковиц, Татјана; Давидовиц, Горан; Јоксимовиц, Ненад; Ранковиц, Бранислав; Косаниц, Маријана; Јанковиц, Ненад; Бугарциц, Зорица;
dc.citation.volume45
dc.citation.issue38
dc.citation.spage15067
dc.citation.epage15077
dc.identifier.wos000385198400025
dc.identifier.doi10.1039/c6dt02257j
dc.citation.rankM21
dc.identifier.pmid27711668
dc.identifier.scopus2-s2.0-84989966778


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record