miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma
Само за регистроване кориснике
2022
Аутори
Todorović, LidijaPetrović, Nina
Mandušić, Vesna
Živaljević, Vladan
Paunović, Ivan
Stanojević, Boban
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Although one of the rarest known malignancies, with an estimated prevalence of 0.005% of all cancers, parathyroid carcinoma (PC) represents a clinical and therapeutic challenge. The identification of novel diagnostic biomarkers able to preoperatively distinguish among different parathyroid neoplastic types is of great clinical importance. However, there is a lack of both experimental and bioinformatics data in this field. In the present study, we examined the expression levels of two microRNAs frequently implicated in cancer, miR-30a-3p and miR-92a-3p, in 10 PC and 10 parathyroid adenoma (PA) tissues using reverse transcription quantitative (RT-qPCR). In addition, we analyzed their association with clinical and histopathological parameters. For statistical analysis we used non-parametric tests – Mann–Whitney U test and Spearman’s correlation test. Furthermore, by using several online tools such as miRNet, miRror Suit, and DisGeNET we analyzed combinatorial target genes of the two miRs ...and investigated their potential roles in parathyroid carcinoma and adenoma. The expression levels of both miR-30a-3p and miR-92a-3p were upregulated in PC compared to PA (p= 0.017 and p=0.0015 respectively). A positive correlation between their expression levels was identified both in PC (p=0.025, r=0.697) and PA (p=0.008, r=0745), indicating their combined action. There were no significant associations between the expression levels of these two miRs and clinicopathological characteristics in the PC group. According to the in silico analysis, the two miRs share a number of target genes, predominantly included in gene expression, cell cycle regulation, and several signalling pathways such as WNT/β catenin, which was found to be frequently aberrant in PC. Although their role and diagnostic utility remain to be elucidated, the data here reported suggest that miR-30a-3p and miR-92a-3p might be involved in parathyroid cancer pathogenesis and that they might be candidates for differential diagnosis between parathyroid carcinoma and adenoma.
Извор:
5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts, 2022Напомена:
- 5th Grant Period Annual Conference; June 2 - July 9, 2022; Coimbra, Portugal
Колекције
Институција/група
VinčaTY - CONF AU - Todorović, Lidija AU - Petrović, Nina AU - Mandušić, Vesna AU - Živaljević, Vladan AU - Paunović, Ivan AU - Stanojević, Boban PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12426 AB - Although one of the rarest known malignancies, with an estimated prevalence of 0.005% of all cancers, parathyroid carcinoma (PC) represents a clinical and therapeutic challenge. The identification of novel diagnostic biomarkers able to preoperatively distinguish among different parathyroid neoplastic types is of great clinical importance. However, there is a lack of both experimental and bioinformatics data in this field. In the present study, we examined the expression levels of two microRNAs frequently implicated in cancer, miR-30a-3p and miR-92a-3p, in 10 PC and 10 parathyroid adenoma (PA) tissues using reverse transcription quantitative (RT-qPCR). In addition, we analyzed their association with clinical and histopathological parameters. For statistical analysis we used non-parametric tests – Mann–Whitney U test and Spearman’s correlation test. Furthermore, by using several online tools such as miRNet, miRror Suit, and DisGeNET we analyzed combinatorial target genes of the two miRs and investigated their potential roles in parathyroid carcinoma and adenoma. The expression levels of both miR-30a-3p and miR-92a-3p were upregulated in PC compared to PA (p= 0.017 and p=0.0015 respectively). A positive correlation between their expression levels was identified both in PC (p=0.025, r=0.697) and PA (p=0.008, r=0745), indicating their combined action. There were no significant associations between the expression levels of these two miRs and clinicopathological characteristics in the PC group. According to the in silico analysis, the two miRs share a number of target genes, predominantly included in gene expression, cell cycle regulation, and several signalling pathways such as WNT/β catenin, which was found to be frequently aberrant in PC. Although their role and diagnostic utility remain to be elucidated, the data here reported suggest that miR-30a-3p and miR-92a-3p might be involved in parathyroid cancer pathogenesis and that they might be candidates for differential diagnosis between parathyroid carcinoma and adenoma. C3 - 5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts T1 - miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma UR - https://hdl.handle.net/21.15107/rcub_vinar_12426 ER -
@conference{ author = "Todorović, Lidija and Petrović, Nina and Mandušić, Vesna and Živaljević, Vladan and Paunović, Ivan and Stanojević, Boban", year = "2022", abstract = "Although one of the rarest known malignancies, with an estimated prevalence of 0.005% of all cancers, parathyroid carcinoma (PC) represents a clinical and therapeutic challenge. The identification of novel diagnostic biomarkers able to preoperatively distinguish among different parathyroid neoplastic types is of great clinical importance. However, there is a lack of both experimental and bioinformatics data in this field. In the present study, we examined the expression levels of two microRNAs frequently implicated in cancer, miR-30a-3p and miR-92a-3p, in 10 PC and 10 parathyroid adenoma (PA) tissues using reverse transcription quantitative (RT-qPCR). In addition, we analyzed their association with clinical and histopathological parameters. For statistical analysis we used non-parametric tests – Mann–Whitney U test and Spearman’s correlation test. Furthermore, by using several online tools such as miRNet, miRror Suit, and DisGeNET we analyzed combinatorial target genes of the two miRs and investigated their potential roles in parathyroid carcinoma and adenoma. The expression levels of both miR-30a-3p and miR-92a-3p were upregulated in PC compared to PA (p= 0.017 and p=0.0015 respectively). A positive correlation between their expression levels was identified both in PC (p=0.025, r=0.697) and PA (p=0.008, r=0745), indicating their combined action. There were no significant associations between the expression levels of these two miRs and clinicopathological characteristics in the PC group. According to the in silico analysis, the two miRs share a number of target genes, predominantly included in gene expression, cell cycle regulation, and several signalling pathways such as WNT/β catenin, which was found to be frequently aberrant in PC. Although their role and diagnostic utility remain to be elucidated, the data here reported suggest that miR-30a-3p and miR-92a-3p might be involved in parathyroid cancer pathogenesis and that they might be candidates for differential diagnosis between parathyroid carcinoma and adenoma.", journal = "5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts", title = "miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma", url = "https://hdl.handle.net/21.15107/rcub_vinar_12426" }
Todorović, L., Petrović, N., Mandušić, V., Živaljević, V., Paunović, I.,& Stanojević, B.. (2022). miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma. in 5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts. https://hdl.handle.net/21.15107/rcub_vinar_12426
Todorović L, Petrović N, Mandušić V, Živaljević V, Paunović I, Stanojević B. miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma. in 5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts. 2022;. https://hdl.handle.net/21.15107/rcub_vinar_12426 .
Todorović, Lidija, Petrović, Nina, Mandušić, Vesna, Živaljević, Vladan, Paunović, Ivan, Stanojević, Boban, "miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma" in 5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts (2022), https://hdl.handle.net/21.15107/rcub_vinar_12426 .