The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis
Uticaj različitih materijala koji oblažu hidroksiapatit koštanog nosača na uspeh regeneracije koštanih defekta kalvarije zeca: histomorfometrijska i histološka analiza
Аутори
Milutinović-Smiljanić, SanjaAntonijević, Đorđe
Mićić, Milutin
Biočanin, Vladimir
Sjerobabin, Nikola
Petrović, Božana
Danilović, Vesna
Jokanović, Vukoman R.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background/Aim. The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and polyethyleneimine (PEI) as coatings for hydroxyapatite (HAP) bone carriers on bone tissue regenerative potential in rabbits? calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP + PLGA), PEI coated HAP (HAP + PEI), and bovine HAP ? Bio-Oss? (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. The formation of the new bone was evaluated using histomorphometric analysis. All analyses have b...een conducted in samples obtained 3, 6, and 9 weeks after implantation. Results. Three weeks post-implantation, a trend toward increased healing in the HAP + PLGA group compared to other investigated materials was noticed, with no statistically significant difference between the study groups (p > 0.05). However, after 6 and 9 weeks, significant healing was observed in favor of the HAP coated with PLGA compared to other groups (p
Uvod/Cilj. Materijali koji se u današnje vreme koriste za nadoknadu koštanog tkiva ne dovode do kompletne regeneracije, zbog čega se ispituju novi. Uprkos mnogobrojnim pokušajima da se poboljša regeneracija koštanog tkiva, još uvek nije pronađen materijal koji ispunjava sve kriterijume. Cilj rada bio je da se utvrdi uticaj poli(laktid-ko-glikolida) (PLGA) i polietilenimina (PEI), kao premaza za oblaganje hidroksiapatita (HAP), na regenerativni potencijal koštanog tkiva u defektu kalvarije zeca. Metode. Kod 19 zečeva „zrelog” skeleta načinjeni su defekti kalvarije dijametra 6 mm. Defekti su potom ispunjeni jednim od sledećih materijala: HAP obložen PLGA (HAP + PLGA), HAP obložen PEI (HAP + PEI) i goveđi HAP – Bio-Oss® (pozitivna kontrola). Prazni defekti su predstavljali negativnu kontrolu. Inflamacijska reakcija tkiva domaćina je ispitana histološkom analizom. Formiranje nove kosti je procenjivano histomorfometrijskom analizom. Analizirani su uzorci dobijeni 3, 6 i 9 nedelja nakon impl...antacije. Rezultati. Tri nedelje nakon implantacije, uočena je tendencija boljeg zarastanja u HAP + PLGA grupi, bez statistički značajne razlike između ispitivanih grupa (p > 0.05). Međutim, 6 i 9 nedelja nakon implantacije, primećeno je značajno formiranje koštanog tkiva u korist HAP + PLGA grupe (p < 0,05). Oblaganje HAP sa PLGA dovelo je do boljeg koštanog zarastanja u poređenju sa HAP+PEI i Bio-Oss®. Zaključak. U pogledu stimulisanja osteogeneze u rekonstruktivnoj hirurgiji kostiju, PLGA je pokazao veći potencijal prekrivanja defekta od PEI.
Кључне речи:
bone regeneration / histological techniques / materials testing / polymers / rabbits / kost, regeneracija / histološke tehnike / materijali, testiranje / polimeri / zečeviИзвор:
Vojnosanitetski pregled, 2022, 79, 10, 1025-1034Финансирање / пројекти:
- Хемијско и структурно дизајнирање наноматеријала за примену у медицини и инжењерству ткива (RS-MESTD-Basic Research (BR or ON)-172026)
- Функционални, функционализовани и усавршени нано материјали (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45005)
DOI: 10.2298/VSP210513072M
ISSN: 0042-8450
WoS: 000973927900011
Scopus: 2-s2.0-85163376760
Институција/група
VinčaTY - JOUR AU - Milutinović-Smiljanić, Sanja AU - Antonijević, Đorđe AU - Mićić, Milutin AU - Biočanin, Vladimir AU - Sjerobabin, Nikola AU - Petrović, Božana AU - Danilović, Vesna AU - Jokanović, Vukoman R. PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/11221 AB - Background/Aim. The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and polyethyleneimine (PEI) as coatings for hydroxyapatite (HAP) bone carriers on bone tissue regenerative potential in rabbits? calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP + PLGA), PEI coated HAP (HAP + PEI), and bovine HAP ? Bio-Oss? (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. The formation of the new bone was evaluated using histomorphometric analysis. All analyses have been conducted in samples obtained 3, 6, and 9 weeks after implantation. Results. Three weeks post-implantation, a trend toward increased healing in the HAP + PLGA group compared to other investigated materials was noticed, with no statistically significant difference between the study groups (p > 0.05). However, after 6 and 9 weeks, significant healing was observed in favor of the HAP coated with PLGA compared to other groups (p AB - Uvod/Cilj. Materijali koji se u današnje vreme koriste za nadoknadu koštanog tkiva ne dovode do kompletne regeneracije, zbog čega se ispituju novi. Uprkos mnogobrojnim pokušajima da se poboljša regeneracija koštanog tkiva, još uvek nije pronađen materijal koji ispunjava sve kriterijume. Cilj rada bio je da se utvrdi uticaj poli(laktid-ko-glikolida) (PLGA) i polietilenimina (PEI), kao premaza za oblaganje hidroksiapatita (HAP), na regenerativni potencijal koštanog tkiva u defektu kalvarije zeca. Metode. Kod 19 zečeva „zrelog” skeleta načinjeni su defekti kalvarije dijametra 6 mm. Defekti su potom ispunjeni jednim od sledećih materijala: HAP obložen PLGA (HAP + PLGA), HAP obložen PEI (HAP + PEI) i goveđi HAP – Bio-Oss® (pozitivna kontrola). Prazni defekti su predstavljali negativnu kontrolu. Inflamacijska reakcija tkiva domaćina je ispitana histološkom analizom. Formiranje nove kosti je procenjivano histomorfometrijskom analizom. Analizirani su uzorci dobijeni 3, 6 i 9 nedelja nakon implantacije. Rezultati. Tri nedelje nakon implantacije, uočena je tendencija boljeg zarastanja u HAP + PLGA grupi, bez statistički značajne razlike između ispitivanih grupa (p > 0.05). Međutim, 6 i 9 nedelja nakon implantacije, primećeno je značajno formiranje koštanog tkiva u korist HAP + PLGA grupe (p < 0,05). Oblaganje HAP sa PLGA dovelo je do boljeg koštanog zarastanja u poređenju sa HAP+PEI i Bio-Oss®. Zaključak. U pogledu stimulisanja osteogeneze u rekonstruktivnoj hirurgiji kostiju, PLGA je pokazao veći potencijal prekrivanja defekta od PEI. T2 - Vojnosanitetski pregled T1 - The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis T1 - Uticaj različitih materijala koji oblažu hidroksiapatit koštanog nosača na uspeh regeneracije koštanih defekta kalvarije zeca: histomorfometrijska i histološka analiza VL - 79 IS - 10 SP - 1025 EP - 1034 DO - 10.2298/VSP210513072M ER -
@article{ author = "Milutinović-Smiljanić, Sanja and Antonijević, Đorđe and Mićić, Milutin and Biočanin, Vladimir and Sjerobabin, Nikola and Petrović, Božana and Danilović, Vesna and Jokanović, Vukoman R.", year = "2022", abstract = "Background/Aim. The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and polyethyleneimine (PEI) as coatings for hydroxyapatite (HAP) bone carriers on bone tissue regenerative potential in rabbits? calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP + PLGA), PEI coated HAP (HAP + PEI), and bovine HAP ? Bio-Oss? (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. The formation of the new bone was evaluated using histomorphometric analysis. All analyses have been conducted in samples obtained 3, 6, and 9 weeks after implantation. Results. Three weeks post-implantation, a trend toward increased healing in the HAP + PLGA group compared to other investigated materials was noticed, with no statistically significant difference between the study groups (p > 0.05). However, after 6 and 9 weeks, significant healing was observed in favor of the HAP coated with PLGA compared to other groups (p, Uvod/Cilj. Materijali koji se u današnje vreme koriste za nadoknadu koštanog tkiva ne dovode do kompletne regeneracije, zbog čega se ispituju novi. Uprkos mnogobrojnim pokušajima da se poboljša regeneracija koštanog tkiva, još uvek nije pronađen materijal koji ispunjava sve kriterijume. Cilj rada bio je da se utvrdi uticaj poli(laktid-ko-glikolida) (PLGA) i polietilenimina (PEI), kao premaza za oblaganje hidroksiapatita (HAP), na regenerativni potencijal koštanog tkiva u defektu kalvarije zeca. Metode. Kod 19 zečeva „zrelog” skeleta načinjeni su defekti kalvarije dijametra 6 mm. Defekti su potom ispunjeni jednim od sledećih materijala: HAP obložen PLGA (HAP + PLGA), HAP obložen PEI (HAP + PEI) i goveđi HAP – Bio-Oss® (pozitivna kontrola). Prazni defekti su predstavljali negativnu kontrolu. Inflamacijska reakcija tkiva domaćina je ispitana histološkom analizom. Formiranje nove kosti je procenjivano histomorfometrijskom analizom. Analizirani su uzorci dobijeni 3, 6 i 9 nedelja nakon implantacije. Rezultati. Tri nedelje nakon implantacije, uočena je tendencija boljeg zarastanja u HAP + PLGA grupi, bez statistički značajne razlike između ispitivanih grupa (p > 0.05). Međutim, 6 i 9 nedelja nakon implantacije, primećeno je značajno formiranje koštanog tkiva u korist HAP + PLGA grupe (p < 0,05). Oblaganje HAP sa PLGA dovelo je do boljeg koštanog zarastanja u poređenju sa HAP+PEI i Bio-Oss®. Zaključak. U pogledu stimulisanja osteogeneze u rekonstruktivnoj hirurgiji kostiju, PLGA je pokazao veći potencijal prekrivanja defekta od PEI.", journal = "Vojnosanitetski pregled", title = "The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis, Uticaj različitih materijala koji oblažu hidroksiapatit koštanog nosača na uspeh regeneracije koštanih defekta kalvarije zeca: histomorfometrijska i histološka analiza", volume = "79", number = "10", pages = "1025-1034", doi = "10.2298/VSP210513072M" }
Milutinović-Smiljanić, S., Antonijević, Đ., Mićić, M., Biočanin, V., Sjerobabin, N., Petrović, B., Danilović, V.,& Jokanović, V. R.. (2022). The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis. in Vojnosanitetski pregled, 79(10), 1025-1034. https://doi.org/10.2298/VSP210513072M
Milutinović-Smiljanić S, Antonijević Đ, Mićić M, Biočanin V, Sjerobabin N, Petrović B, Danilović V, Jokanović VR. The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis. in Vojnosanitetski pregled. 2022;79(10):1025-1034. doi:10.2298/VSP210513072M .
Milutinović-Smiljanić, Sanja, Antonijević, Đorđe, Mićić, Milutin, Biočanin, Vladimir, Sjerobabin, Nikola, Petrović, Božana, Danilović, Vesna, Jokanović, Vukoman R., "The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis" in Vojnosanitetski pregled, 79, no. 10 (2022):1025-1034, https://doi.org/10.2298/VSP210513072M . .