Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)
Аутори
Živković, LadaČabarkapa, Andrea
Marčetić, Mirjana
Kovačević, Nada
Bajić, Vladan P.
Jovičić, Snežana
Spremo-Potparević, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. B...ased on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.
Кључне речи:
Seseli rigidum / essential oils / antioxidant / DNA damage / comet assayИзвор:
Archives of Biological Sciences, 2016, 68, 1, 135-144Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-Basic Research (BR or ON)-173021)
DOI: 10.2298/ABS150512135Z
ISSN: 0354-4664; 1821-4339
WoS: 000377883400014
Scopus: 2-s2.0-84961626633
Колекције
Институција/група
VinčaTY - JOUR AU - Živković, Lada AU - Čabarkapa, Andrea AU - Marčetić, Mirjana AU - Kovačević, Nada AU - Bajić, Vladan P. AU - Jovičić, Snežana AU - Spremo-Potparević, Biljana PY - 2016 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1121 AB - The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions. T2 - Archives of Biological Sciences T1 - Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae) VL - 68 IS - 1 SP - 135 EP - 144 DO - 10.2298/ABS150512135Z ER -
@article{ author = "Živković, Lada and Čabarkapa, Andrea and Marčetić, Mirjana and Kovačević, Nada and Bajić, Vladan P. and Jovičić, Snežana and Spremo-Potparević, Biljana", year = "2016", abstract = "The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.", journal = "Archives of Biological Sciences", title = "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)", volume = "68", number = "1", pages = "135-144", doi = "10.2298/ABS150512135Z" }
Živković, L., Čabarkapa, A., Marčetić, M., Kovačević, N., Bajić, V. P., Jovičić, S.,& Spremo-Potparević, B.. (2016). Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae). in Archives of Biological Sciences, 68(1), 135-144. https://doi.org/10.2298/ABS150512135Z
Živković L, Čabarkapa A, Marčetić M, Kovačević N, Bajić VP, Jovičić S, Spremo-Potparević B. Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae). in Archives of Biological Sciences. 2016;68(1):135-144. doi:10.2298/ABS150512135Z .
Živković, Lada, Čabarkapa, Andrea, Marčetić, Mirjana, Kovačević, Nada, Bajić, Vladan P., Jovičić, Snežana, Spremo-Potparević, Biljana, "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)" in Archives of Biological Sciences, 68, no. 1 (2016):135-144, https://doi.org/10.2298/ABS150512135Z . .