Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
Authors
Stojanović, MarijaScepanovic, Ljiljana
Bosnic, Olivera
Mitrovic, Dusan
Jozanov-Stankov, Olga
Scepanovic, Vuk
Scepanovic, Radomir
Stojanovic, Teja
Ilic, Slobodan
Đurić, Dragan M.
Article (Published version)
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Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in ho...mogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.
Keywords:
antioxidant enzymes / homocysteine / lipid peroxidation / oxidative stressSource:
Acta Veterinaria, Beograd, 2016, 66, 1, 26-36Funding / projects:
- The effects of homocysteine and homocysteine-related compounds on cardiovascular system: role of gaseous transmitters No, H2S and CO (RS-MESTD-Basic Research (BR or ON)-175043)
DOI: 10.1515/acve-2016-0002
ISSN: 0567-8315; 1820-7448
WoS: 000377780400002
Scopus: 2-s2.0-84962784582
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VinčaTY - JOUR AU - Stojanović, Marija AU - Scepanovic, Ljiljana AU - Bosnic, Olivera AU - Mitrovic, Dusan AU - Jozanov-Stankov, Olga AU - Scepanovic, Vuk AU - Scepanovic, Radomir AU - Stojanovic, Teja AU - Ilic, Slobodan AU - Đurić, Dragan M. PY - 2016 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1117 AB - Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine. T2 - Acta Veterinaria, Beograd T1 - Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver VL - 66 IS - 1 SP - 26 EP - 36 DO - 10.1515/acve-2016-0002 ER -
@article{ author = "Stojanović, Marija and Scepanovic, Ljiljana and Bosnic, Olivera and Mitrovic, Dusan and Jozanov-Stankov, Olga and Scepanovic, Vuk and Scepanovic, Radomir and Stojanovic, Teja and Ilic, Slobodan and Đurić, Dragan M.", year = "2016", abstract = "Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.", journal = "Acta Veterinaria, Beograd", title = "Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver", volume = "66", number = "1", pages = "26-36", doi = "10.1515/acve-2016-0002" }
Stojanović, M., Scepanovic, L., Bosnic, O., Mitrovic, D., Jozanov-Stankov, O., Scepanovic, V., Scepanovic, R., Stojanovic, T., Ilic, S.,& Đurić, D. M.. (2016). Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver. in Acta Veterinaria, Beograd, 66(1), 26-36. https://doi.org/10.1515/acve-2016-0002
Stojanović M, Scepanovic L, Bosnic O, Mitrovic D, Jozanov-Stankov O, Scepanovic V, Scepanovic R, Stojanovic T, Ilic S, Đurić DM. Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver. in Acta Veterinaria, Beograd. 2016;66(1):26-36. doi:10.1515/acve-2016-0002 .
Stojanović, Marija, Scepanovic, Ljiljana, Bosnic, Olivera, Mitrovic, Dusan, Jozanov-Stankov, Olga, Scepanovic, Vuk, Scepanovic, Radomir, Stojanovic, Teja, Ilic, Slobodan, Đurić, Dragan M., "Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver" in Acta Veterinaria, Beograd, 66, no. 1 (2016):26-36, https://doi.org/10.1515/acve-2016-0002 . .