Downregulation of fibrosis related hsa-miR-29c-3p in human CAKUT
Нема приказа
Аутори
Mačak, NatašaJovanović, Ivan G.
Živković, Maja
Mitrović, Kristina
Cvetković, Mirjana
Kostić, Mirjana
Stanković, Aleksandra
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Congenital anomalies of the kidney and urinary tract (CAKUT) represent structural and functional urinary system malformations and take place as one of the most common congenital malformations with an incidence of 1:500. Ureteral obstruction-induced hydronephrosis is associated with renal fibrosis and chronic kidney diseases in the pediatric CAKUT. We aimed to construct interaction network of previously bioinformatically associated miRNAs with CAKUT differentially expressed genes in order to prioritize those associated with fibrotic process and to experimentally validate the expression of selected miRNAs in CAKUT patients compared to control group. We constructed interaction network of hsa-miR-101-3p, hsa-miR-101-5p and hsa-miR-29c-3p that showed significant association with fibrosis. The top enriched molecular pathway was extracellular matrix-receptor interaction (adjusted p = .0000263). We experimentally confirmed expression of three miRNAs (hsa-miR-29c-3p, hsa-miR-101-3p and hsa-miR-...101-5p) in obstructed ureters (ureteropelvic junction obstruction and primary obstructive megaureter) and vesicoureteral reflux. The hsa-miR-29c-3p was shown to have lower expression in both patient groups compared to controls. Relative levels of hsa-miR-101-5p and hsa-miR-101-3p showed significant positive correlations in both groups of patients. Statistically significant correlation was observed between hsa-miR-101 (-3p and -5p) and hsa-miR-29c-3p only in the obstructed group. The significant downregulation of anti-fibrotic hsa-miR-29c-3p in obstructive CAKUT could explain activation of genes involved in fibrotic processes. As miRNAs are promising candidates in therapeutic approaches our results need further measurement of fibrotic markers or assessment of extent of fibrosis and functional evaluation of hsa-miR-29c.
Кључне речи:
Bioinformatics / CAKUT / fibrosis / miR-101 / miR-29cИзвор:
Nucleosides, Nucleotides & Nucleic Acids, 2023, 42, 12, 945-958
DOI: 10.1080/15257770.2023.2218430
ISSN: 1525-7770
PubMed: 3729187
Scopus: 2-s2.0-85161634707
Институција/група
VinčaTY - JOUR AU - Mačak, Nataša AU - Jovanović, Ivan G. AU - Živković, Maja AU - Mitrović, Kristina AU - Cvetković, Mirjana AU - Kostić, Mirjana AU - Stanković, Aleksandra PY - 2023 UR - https://vinar.vin.bg.ac.rs/handle/123456789/11165 AB - Congenital anomalies of the kidney and urinary tract (CAKUT) represent structural and functional urinary system malformations and take place as one of the most common congenital malformations with an incidence of 1:500. Ureteral obstruction-induced hydronephrosis is associated with renal fibrosis and chronic kidney diseases in the pediatric CAKUT. We aimed to construct interaction network of previously bioinformatically associated miRNAs with CAKUT differentially expressed genes in order to prioritize those associated with fibrotic process and to experimentally validate the expression of selected miRNAs in CAKUT patients compared to control group. We constructed interaction network of hsa-miR-101-3p, hsa-miR-101-5p and hsa-miR-29c-3p that showed significant association with fibrosis. The top enriched molecular pathway was extracellular matrix-receptor interaction (adjusted p = .0000263). We experimentally confirmed expression of three miRNAs (hsa-miR-29c-3p, hsa-miR-101-3p and hsa-miR-101-5p) in obstructed ureters (ureteropelvic junction obstruction and primary obstructive megaureter) and vesicoureteral reflux. The hsa-miR-29c-3p was shown to have lower expression in both patient groups compared to controls. Relative levels of hsa-miR-101-5p and hsa-miR-101-3p showed significant positive correlations in both groups of patients. Statistically significant correlation was observed between hsa-miR-101 (-3p and -5p) and hsa-miR-29c-3p only in the obstructed group. The significant downregulation of anti-fibrotic hsa-miR-29c-3p in obstructive CAKUT could explain activation of genes involved in fibrotic processes. As miRNAs are promising candidates in therapeutic approaches our results need further measurement of fibrotic markers or assessment of extent of fibrosis and functional evaluation of hsa-miR-29c. T2 - Nucleosides, Nucleotides & Nucleic Acids T1 - Downregulation of fibrosis related hsa-miR-29c-3p in human CAKUT VL - 42 IS - 12 SP - 945 EP - 958 DO - 10.1080/15257770.2023.2218430 ER -
@article{ author = "Mačak, Nataša and Jovanović, Ivan G. and Živković, Maja and Mitrović, Kristina and Cvetković, Mirjana and Kostić, Mirjana and Stanković, Aleksandra", year = "2023", abstract = "Congenital anomalies of the kidney and urinary tract (CAKUT) represent structural and functional urinary system malformations and take place as one of the most common congenital malformations with an incidence of 1:500. Ureteral obstruction-induced hydronephrosis is associated with renal fibrosis and chronic kidney diseases in the pediatric CAKUT. We aimed to construct interaction network of previously bioinformatically associated miRNAs with CAKUT differentially expressed genes in order to prioritize those associated with fibrotic process and to experimentally validate the expression of selected miRNAs in CAKUT patients compared to control group. We constructed interaction network of hsa-miR-101-3p, hsa-miR-101-5p and hsa-miR-29c-3p that showed significant association with fibrosis. The top enriched molecular pathway was extracellular matrix-receptor interaction (adjusted p = .0000263). We experimentally confirmed expression of three miRNAs (hsa-miR-29c-3p, hsa-miR-101-3p and hsa-miR-101-5p) in obstructed ureters (ureteropelvic junction obstruction and primary obstructive megaureter) and vesicoureteral reflux. The hsa-miR-29c-3p was shown to have lower expression in both patient groups compared to controls. Relative levels of hsa-miR-101-5p and hsa-miR-101-3p showed significant positive correlations in both groups of patients. Statistically significant correlation was observed between hsa-miR-101 (-3p and -5p) and hsa-miR-29c-3p only in the obstructed group. The significant downregulation of anti-fibrotic hsa-miR-29c-3p in obstructive CAKUT could explain activation of genes involved in fibrotic processes. As miRNAs are promising candidates in therapeutic approaches our results need further measurement of fibrotic markers or assessment of extent of fibrosis and functional evaluation of hsa-miR-29c.", journal = "Nucleosides, Nucleotides & Nucleic Acids", title = "Downregulation of fibrosis related hsa-miR-29c-3p in human CAKUT", volume = "42", number = "12", pages = "945-958", doi = "10.1080/15257770.2023.2218430" }
Mačak, N., Jovanović, I. G., Živković, M., Mitrović, K., Cvetković, M., Kostić, M.,& Stanković, A.. (2023). Downregulation of fibrosis related hsa-miR-29c-3p in human CAKUT. in Nucleosides, Nucleotides & Nucleic Acids, 42(12), 945-958. https://doi.org/10.1080/15257770.2023.2218430
Mačak N, Jovanović IG, Živković M, Mitrović K, Cvetković M, Kostić M, Stanković A. Downregulation of fibrosis related hsa-miR-29c-3p in human CAKUT. in Nucleosides, Nucleotides & Nucleic Acids. 2023;42(12):945-958. doi:10.1080/15257770.2023.2218430 .
Mačak, Nataša, Jovanović, Ivan G., Živković, Maja, Mitrović, Kristina, Cvetković, Mirjana, Kostić, Mirjana, Stanković, Aleksandra, "Downregulation of fibrosis related hsa-miR-29c-3p in human CAKUT" in Nucleosides, Nucleotides & Nucleic Acids, 42, no. 12 (2023):945-958, https://doi.org/10.1080/15257770.2023.2218430 . .