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dc.creatorPaunović, Verica G.
dc.creatorRistic, Biljana
dc.creatorMarković, Zoran M.
dc.creatorTodorović-Marković, Biljana
dc.creatorKosic, Milica
dc.creatorPrekodravac, Jovana
dc.creatorKravić-Stevović, Tamara K.
dc.creatorMartinović, Tamara
dc.creatorMičušik, Matej
dc.creatorŠpitalsky, Zdenko
dc.creatorTrajković, Vladimir S.
dc.creatorHarhaji-Trajković, Ljubica M.
dc.date.accessioned2018-03-01T16:54:44Z
dc.date.available2018-03-01T16:54:44Z
dc.date.issued2016
dc.identifier.issn1387-2176 (print)
dc.identifier.issn1572-8781 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1084
dc.description.abstractIndian spice curcumin is known for its anticancer properties, but the anticancer mechanisms of nanoparticulate curcumin have not been completely elucidated. We here investigated the in vitro anticancer effect of blue light (470 nm, 1 W)-irradiated curcumin nanoparticles prepared by tetrahydrofuran/water solvent exchange, using U251 glioma, B16 melanoma, and H460 lung cancer cells as targets. The size of curcumin nanocrystals was approximately 250 nm, while photoexcitation induced their oxidation and partial agglomeration. Although cell membrane in the absence of light was almost impermeable to curcumin nanoparticles, photoexcitation stimulated their internalization. While irradiation with blue light (1-8 min) or nanocurcumin (1.25-10 mu g/ml) alone was only marginally toxic to tumor cells, photoexcited nanocurcumin displayed a significant cytotoxicity depending both on the irradiation time and nanocurcumin concentration. Photoexcited nanocurcumin induced phosphorylation of cJun N-terminal kinase (JNK), mitochondrial depolarization, caspase-3 activation, and cleavage of poly (ADP-ribose) polymerase, indicating apoptotic cell death. Accordingly, pharmacologial inhibition of JNK and caspase activity rescued cancer cells from photoexcited nanocurcumin. On the other hand, antioxidant treatment did not reduce photocytotoxicity of nanocurcumin, arguing against the involvement of oxidative stress. By demonstrating the ability of photoexcited nanocurcumin to induce oxidative-stress independent, JNK-and caspase-dependent apoptosis, our results support its further investigation in cancer therapy.en
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/609427/EU//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41025/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172003/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173053/RS//
dc.relationSASPRO Program project [1237/02/02-b], Slovak Academy of Sciences
dc.rightsrestrictedAccessen
dc.sourceBiomedical Microdevicesen
dc.subjectNanocurcuminen
dc.subjectPhotoexcitationen
dc.subjectCanceren
dc.subjectApoptosisen
dc.subjectJNKen
dc.titlec-Jun N-terminal kinase-dependent apoptotic photocytotoxicity of solvent exchange-prepared curcumin nanoparticlesen
dc.typearticleen
dcterms.abstractРистиц, Биљана; Марковић Зоран; Кравиц-Стевовиц, Тамара; Тодоровић-Марковић Биљана; Мицусик, Матеј; Косиц, Милица; Пауновиц, Верица; Прекодравац Јована; Мартиновиц, Тамара; Спиталскy, Здено; Трајковиц, Владимир; Хархаји-Трајковиц, Љубица;
dc.citation.volume18
dc.citation.issue2
dc.identifier.wos000376579800015
dc.identifier.doi10.1007/s10544-016-0062-2
dc.citation.otherArticle Number: 37
dc.citation.rankM21
dc.identifier.pmid27106025
dc.identifier.scopus2-s2.0-84964241091


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