Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots
Authors
Jovanović, Svetlana P.Bonasera, Aurelio
Dorontić, Slađana
Zmejkoski, Danica
Milivojević, Dušan
Janakiev, Tamara
Todorović-Marković, Biljana
Article (Published version)
Metadata
Show full item recordAbstract
Due to the increasing number of bacterial infections and the development of resistivity toward antibiotics, new materials and approaches for treatments must be urgently developed. The production of new materials should be ecologically friendly considering overall pollution with chemicals and economically acceptable and accessible to the wide population. Thus, the possibility of using biocompatible graphene quantum dots (GQDs) as an agent in photodynamic therapy was studied. First, dots were obtained using electrochemical cutting of graphite. In only one synthetic step using gamma irradiation, GQDs were doped with N atoms without any reagent. Obtained dots showed blue photoluminescence, with a diameter of 19–89 nm and optical band gap of 3.23–4.73 eV, featuring oxygen-containing, amino, and amide functional groups. Dots showed antioxidative activity; they quenched •OH at a concentration of 10 μg·mL−1, scavenged DPPH• radicals even at 5 μg·mL−1, and caused discoloration of KMnO4 at 30 μg...·mL−1. Under light irradiation, dots were able to produce singlet oxygen, which remained stable for 10 min. Photoinduced effects by GQDs were studied on several bacterial strains (Listeria monocytogenes, Bacillus cereus, clinical strains of Streptococcus mutans, S. pyogenes, and S. sangunis, Pseudomonas aeruginosa, and one yeast strain Candida albicans) but antibacterial effects were not noticed.
Keywords:
antibacterial effects / antioxidant / gamma-irradiation / graphene quantum dots / N-doping / photodynamic therapy / photoluminescenceSource:
Materials, 2022, 15, 19, 6525-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-MESTD-inst-2020-200017)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
Institution/Community
VinčaTY - JOUR AU - Jovanović, Svetlana P. AU - Bonasera, Aurelio AU - Dorontić, Slađana AU - Zmejkoski, Danica AU - Milivojević, Dušan AU - Janakiev, Tamara AU - Todorović-Marković, Biljana PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10633 AB - Due to the increasing number of bacterial infections and the development of resistivity toward antibiotics, new materials and approaches for treatments must be urgently developed. The production of new materials should be ecologically friendly considering overall pollution with chemicals and economically acceptable and accessible to the wide population. Thus, the possibility of using biocompatible graphene quantum dots (GQDs) as an agent in photodynamic therapy was studied. First, dots were obtained using electrochemical cutting of graphite. In only one synthetic step using gamma irradiation, GQDs were doped with N atoms without any reagent. Obtained dots showed blue photoluminescence, with a diameter of 19–89 nm and optical band gap of 3.23–4.73 eV, featuring oxygen-containing, amino, and amide functional groups. Dots showed antioxidative activity; they quenched •OH at a concentration of 10 μg·mL−1, scavenged DPPH• radicals even at 5 μg·mL−1, and caused discoloration of KMnO4 at 30 μg·mL−1. Under light irradiation, dots were able to produce singlet oxygen, which remained stable for 10 min. Photoinduced effects by GQDs were studied on several bacterial strains (Listeria monocytogenes, Bacillus cereus, clinical strains of Streptococcus mutans, S. pyogenes, and S. sangunis, Pseudomonas aeruginosa, and one yeast strain Candida albicans) but antibacterial effects were not noticed. T2 - Materials T1 - Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots VL - 15 IS - 19 SP - 6525 DO - 10.3390/ma15196525 ER -
@article{ author = "Jovanović, Svetlana P. and Bonasera, Aurelio and Dorontić, Slađana and Zmejkoski, Danica and Milivojević, Dušan and Janakiev, Tamara and Todorović-Marković, Biljana", year = "2022", abstract = "Due to the increasing number of bacterial infections and the development of resistivity toward antibiotics, new materials and approaches for treatments must be urgently developed. The production of new materials should be ecologically friendly considering overall pollution with chemicals and economically acceptable and accessible to the wide population. Thus, the possibility of using biocompatible graphene quantum dots (GQDs) as an agent in photodynamic therapy was studied. First, dots were obtained using electrochemical cutting of graphite. In only one synthetic step using gamma irradiation, GQDs were doped with N atoms without any reagent. Obtained dots showed blue photoluminescence, with a diameter of 19–89 nm and optical band gap of 3.23–4.73 eV, featuring oxygen-containing, amino, and amide functional groups. Dots showed antioxidative activity; they quenched •OH at a concentration of 10 μg·mL−1, scavenged DPPH• radicals even at 5 μg·mL−1, and caused discoloration of KMnO4 at 30 μg·mL−1. Under light irradiation, dots were able to produce singlet oxygen, which remained stable for 10 min. Photoinduced effects by GQDs were studied on several bacterial strains (Listeria monocytogenes, Bacillus cereus, clinical strains of Streptococcus mutans, S. pyogenes, and S. sangunis, Pseudomonas aeruginosa, and one yeast strain Candida albicans) but antibacterial effects were not noticed.", journal = "Materials", title = "Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots", volume = "15", number = "19", pages = "6525", doi = "10.3390/ma15196525" }
Jovanović, S. P., Bonasera, A., Dorontić, S., Zmejkoski, D., Milivojević, D., Janakiev, T.,& Todorović-Marković, B.. (2022). Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots. in Materials, 15(19), 6525. https://doi.org/10.3390/ma15196525
Jovanović SP, Bonasera A, Dorontić S, Zmejkoski D, Milivojević D, Janakiev T, Todorović-Marković B. Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots. in Materials. 2022;15(19):6525. doi:10.3390/ma15196525 .
Jovanović, Svetlana P., Bonasera, Aurelio, Dorontić, Slađana, Zmejkoski, Danica, Milivojević, Dušan, Janakiev, Tamara, Todorović-Marković, Biljana, "Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots" in Materials, 15, no. 19 (2022):6525, https://doi.org/10.3390/ma15196525 . .