Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection
Само за регистроване кориснике
2022
Аутори
Đurđić, SlađanaOgnjanović, Miloš
Krstić Ristivojević, Maja
Antić, Bratislav
Ćirković Veličković, Tanja
Mutić, Jelena
Kónya, Zoltán
Stanković, Dalibor M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
An electrochemical approach is presented based on multiwall carbon nanotubes (MWCNTs) and neodymium(III) hydroxide (Nd(OH)3) nanoflakes for detection of bovine serum albumin (BSA). The materials were characterized morphologically (XRPD, SEM, and HR-TEM) and electrochemically (DPV, EIS). The MWCNTs@Nd(OH)3 composite was used as support for bovine serum albumin polyclonal antibody (anti-BSA). After the antibody immobilization on the electrochemical platform and antigen/antibody binding time (optimum 60 min), the proposed approach shows a linear voltammetric response toward BSA concentration in the range 0.066 to 6.010 ng mL−1 at maximum peak potential of 0.13 V (vs. Ag/AgCl). Limit of detection (LOD) and limit of quantification (LOQ) were 18 pg mL−1 and 61 pg mL−1, respectively. The precision of the method calculated as relative standard deviation (RSD) of five independent measurements was better 3%. The selectivity of the optimized method regarding structurally similar proteins (human s...erum albumin and human hemoglobin), ions (Na+, K+, Ca2+, and NO2−), or compounds (glucose, ascorbic acid, dopamine, uric acid, paracetamol, and glycine) was found to be satisfactory, with the current changes of less than 5% in the presence of up to 1 × 105 times higher concentrations (depending on the compound) of the listed potential interfering compounds. Practical applicability of immunosensor for BSA determination in cow whey sample, with recovery values in the range 97 to 103%, shows that the developed method has high potential for precise and accurate detection of BSA, as well as exceptional miniaturization possibilities for on-site and equipment-free sensing.
Кључне речи:
Bovine serum albumin / Bovine serum albumin polyclonal antibody / Differential pulse voltammetry / Electrochemical immunosensor / Multi-wall carbon nanotubes / Neodymium(III) hydroxideИзвор:
Microchimica Acta, 2022, 189, 11, 422-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- Eureka [project E! 13303 MED-BIO-TEST]
- Ministry of Education, Science and Technological Development of Republic of Serbia [contract number 451-03-00053/2020-09/2/2]
DOI: 10.1007/s00604-022-05514-z
ISSN: 1436-5073
PubMed: 36253569
Scopus: 2-s2.0-85140047661
Колекције
Институција/група
VinčaTY - JOUR AU - Đurđić, Slađana AU - Ognjanović, Miloš AU - Krstić Ristivojević, Maja AU - Antić, Bratislav AU - Ćirković Veličković, Tanja AU - Mutić, Jelena AU - Kónya, Zoltán AU - Stanković, Dalibor M. PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10471 AB - An electrochemical approach is presented based on multiwall carbon nanotubes (MWCNTs) and neodymium(III) hydroxide (Nd(OH)3) nanoflakes for detection of bovine serum albumin (BSA). The materials were characterized morphologically (XRPD, SEM, and HR-TEM) and electrochemically (DPV, EIS). The MWCNTs@Nd(OH)3 composite was used as support for bovine serum albumin polyclonal antibody (anti-BSA). After the antibody immobilization on the electrochemical platform and antigen/antibody binding time (optimum 60 min), the proposed approach shows a linear voltammetric response toward BSA concentration in the range 0.066 to 6.010 ng mL−1 at maximum peak potential of 0.13 V (vs. Ag/AgCl). Limit of detection (LOD) and limit of quantification (LOQ) were 18 pg mL−1 and 61 pg mL−1, respectively. The precision of the method calculated as relative standard deviation (RSD) of five independent measurements was better 3%. The selectivity of the optimized method regarding structurally similar proteins (human serum albumin and human hemoglobin), ions (Na+, K+, Ca2+, and NO2−), or compounds (glucose, ascorbic acid, dopamine, uric acid, paracetamol, and glycine) was found to be satisfactory, with the current changes of less than 5% in the presence of up to 1 × 105 times higher concentrations (depending on the compound) of the listed potential interfering compounds. Practical applicability of immunosensor for BSA determination in cow whey sample, with recovery values in the range 97 to 103%, shows that the developed method has high potential for precise and accurate detection of BSA, as well as exceptional miniaturization possibilities for on-site and equipment-free sensing. T2 - Microchimica Acta T1 - Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection VL - 189 IS - 11 SP - 422 DO - 10.1007/s00604-022-05514-z ER -
@article{ author = "Đurđić, Slađana and Ognjanović, Miloš and Krstić Ristivojević, Maja and Antić, Bratislav and Ćirković Veličković, Tanja and Mutić, Jelena and Kónya, Zoltán and Stanković, Dalibor M.", year = "2022", abstract = "An electrochemical approach is presented based on multiwall carbon nanotubes (MWCNTs) and neodymium(III) hydroxide (Nd(OH)3) nanoflakes for detection of bovine serum albumin (BSA). The materials were characterized morphologically (XRPD, SEM, and HR-TEM) and electrochemically (DPV, EIS). The MWCNTs@Nd(OH)3 composite was used as support for bovine serum albumin polyclonal antibody (anti-BSA). After the antibody immobilization on the electrochemical platform and antigen/antibody binding time (optimum 60 min), the proposed approach shows a linear voltammetric response toward BSA concentration in the range 0.066 to 6.010 ng mL−1 at maximum peak potential of 0.13 V (vs. Ag/AgCl). Limit of detection (LOD) and limit of quantification (LOQ) were 18 pg mL−1 and 61 pg mL−1, respectively. The precision of the method calculated as relative standard deviation (RSD) of five independent measurements was better 3%. The selectivity of the optimized method regarding structurally similar proteins (human serum albumin and human hemoglobin), ions (Na+, K+, Ca2+, and NO2−), or compounds (glucose, ascorbic acid, dopamine, uric acid, paracetamol, and glycine) was found to be satisfactory, with the current changes of less than 5% in the presence of up to 1 × 105 times higher concentrations (depending on the compound) of the listed potential interfering compounds. Practical applicability of immunosensor for BSA determination in cow whey sample, with recovery values in the range 97 to 103%, shows that the developed method has high potential for precise and accurate detection of BSA, as well as exceptional miniaturization possibilities for on-site and equipment-free sensing.", journal = "Microchimica Acta", title = "Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection", volume = "189", number = "11", pages = "422", doi = "10.1007/s00604-022-05514-z" }
Đurđić, S., Ognjanović, M., Krstić Ristivojević, M., Antić, B., Ćirković Veličković, T., Mutić, J., Kónya, Z.,& Stanković, D. M.. (2022). Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection. in Microchimica Acta, 189(11), 422. https://doi.org/10.1007/s00604-022-05514-z
Đurđić S, Ognjanović M, Krstić Ristivojević M, Antić B, Ćirković Veličković T, Mutić J, Kónya Z, Stanković DM. Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection. in Microchimica Acta. 2022;189(11):422. doi:10.1007/s00604-022-05514-z .
Đurđić, Slađana, Ognjanović, Miloš, Krstić Ristivojević, Maja, Antić, Bratislav, Ćirković Veličković, Tanja, Mutić, Jelena, Kónya, Zoltán, Stanković, Dalibor M., "Voltammetric immunoassay based on MWCNTs@Nd(OH)3-BSA-antibody platform for sensitive BSA detection" in Microchimica Acta, 189, no. 11 (2022):422, https://doi.org/10.1007/s00604-022-05514-z . .