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dc.creatorKeta, Otilija D.
dc.creatorBulat, Tanja M.
dc.creatorGolic, Igor
dc.creatorIncerti, Sebastien
dc.creatorKorac, Aleksandra
dc.creatorPetrović, Ivan M.
dc.creatorRistić-Fira, Aleksandra
dc.date.accessioned2018-03-01T16:51:15Z
dc.date.available2018-03-01T16:51:15Z
dc.date.issued2016
dc.identifier.issn0742-2091 (print)
dc.identifier.issn1573-6822 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1044
dc.description.abstractIn most patients with lung cancer radiation treatment is used either as single agent or in combination with radiosensitizing drugs. However, the mechanisms underlying combined therapy and its impact on different modes of cell death have not yet been fully elucidated. We aimed to examine effects of single and combined treatments with gamma-rays and erlotinib on radioresistant CRL-5876 human lung adenocarcinoma cells with particular emphasis on cell death. CRL-5876 cells were treated with gamma-rays and/or erlotinib and changes in cell cycle, DNA repair dynamics, ultrastructure, nuclear morphology and protein expression were monitored at different time points. To reveal the relationship between types of cell death that arise after these treatments, autophagy was blocked with chloroquine. We found that higher dose of gamma-rays causes G2/M arrest while adding of erlotinib to this treatment decreases the number of cells in S phase. Impact of erlotinib on kinetics of disappearance of irradiation-induced DNA double strand breaks is reflected in the increase of residual gamma-H2AX foci after 24 h. gamma-rays provoke cytoprotective autophagy which precedes development of senescence. Erlotinib predominantly induces apoptosis and enlarges the number of apoptotic cells in the irradiated CRL-5876 cells. Chloroquine improved cytotoxicity induced by radiation and erlotinib, increased apoptosis and decreased senescence in the CRL-5876 cells. The results obtained on CRL-5876 cells indicate significant radiosensitizing effect of erlotinib and suggest that chloroquine in the combination with the above treatments may have an additional antitumor effect in lung adenocarcinoma.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173046/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/171019/RS//
dc.rightsrestrictedAccessen
dc.sourceCell Biology and Toxicologyen
dc.subjectApoptosisen
dc.subjectAutophagyen
dc.subjectErlotiniben
dc.subjectgamma-raysen
dc.subjectLung adenocarcinomaen
dc.subjectSenescenceen
dc.titleThe impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotiniben
dc.typearticleen
dcterms.abstractКета, Отилија; Булат Тања М.; Корац, Aлександра; Голиц, Игор; Инцерти, Себастиен; Петровић Иван; Ристић-Фира Aлександра;
dc.citation.volume32
dc.citation.issue2
dc.citation.spage83
dc.citation.epage101
dc.identifier.wos000374973400001
dc.identifier.doi10.1007/s10565-016-9319-z
dc.citation.rankM22
dc.identifier.pmid27026538
dc.identifier.scopus2-s2.0-84962310141


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