Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium
Аутори
Gavrilović, LjubicaPopović, Nataša
Stojiljković, Vesna
Pejić, Snežana
Todorović, Ana
Vujović, Predrag
Pajović, Snežana B.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understa...nding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.
Кључне речи:
Antioxidant enzymes / Prefrontal cortex / Lithium / Chronic restraint stress / RatsИзвор:
PeerJ, 2022, 10, e13020-Финансирање / пројекти:
DOI: 10.7717/peerj.13020
ISSN: 2167-8359
WoS: 000777565600003
Scopus: 2-s2.0-85127526817
Институција/група
VinčaTY - JOUR AU - Gavrilović, Ljubica AU - Popović, Nataša AU - Stojiljković, Vesna AU - Pejić, Snežana AU - Todorović, Ana AU - Vujović, Predrag AU - Pajović, Snežana B. PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10235 AB - Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress. T2 - PeerJ T1 - Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium VL - 10 SP - e13020 DO - 10.7717/peerj.13020 ER -
@article{ author = "Gavrilović, Ljubica and Popović, Nataša and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Vujović, Predrag and Pajović, Snežana B.", year = "2022", abstract = "Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.", journal = "PeerJ", title = "Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium", volume = "10", pages = "e13020", doi = "10.7717/peerj.13020" }
Gavrilović, L., Popović, N., Stojiljković, V., Pejić, S., Todorović, A., Vujović, P.,& Pajović, S. B.. (2022). Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium. in PeerJ, 10, e13020. https://doi.org/10.7717/peerj.13020
Gavrilović L, Popović N, Stojiljković V, Pejić S, Todorović A, Vujović P, Pajović SB. Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium. in PeerJ. 2022;10:e13020. doi:10.7717/peerj.13020 .
Gavrilović, Ljubica, Popović, Nataša, Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Vujović, Predrag, Pajović, Snežana B., "Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium" in PeerJ, 10 (2022):e13020, https://doi.org/10.7717/peerj.13020 . .