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dc.creatorMitrović, Nataša Lj.
dc.creatorZarić, Marina
dc.creatorDrakulić, Dunja R.
dc.creatorMartinović, Jelena
dc.creatorStanojlović, Miloš R.
dc.creatorSevigny, J.
dc.creatorHorvat, Anica
dc.creatorNedeljkovic, N.
dc.creatorGrković, Ivana
dc.date.accessioned2018-03-01T16:49:18Z
dc.date.available2018-03-01T16:49:18Z
dc.date.issued2016
dc.identifier.issn0306-4522 (print)
dc.identifier.issn1873-7544 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1022
dc.description.abstract17 beta-Estradiol (E2) crucially affects several processes in the hippocampus of both sexes. E2 acts upon estradiol receptors ER alpha and ER beta, influencing target gene expression and/or modulates intracellular signaling cascades. Another potent modulator of hippocampal function is nucleoside adenosine, the final product of ectonucleoti-dase cascade, enzymes which hydrolyze extracellular ATP to adenosine. The last and rate-limiting step of the hydrolysis is catalyzed by membrane-bound ecto-50-nucleotidase (eN). Previous findings obtained on adenosine metabolism in brain suggest that eN may be modulated by ovarian steroids. Therefore, the present study reports that the activity and protein abundance of membrane-bound eN fluctuates across the estrus cycle in the hippocampal synaptosomes of female rats. Further, we analyzed the role of E2 and its intracellular receptors on the expression of eN in ovariectomized females. We found that E2 upregulated eN activity and protein abundance in the hippocampal synaptosomes. Application of nonspecific ER antagonist, ICI 182,780 and selective ERa and ERb agonists, PPT and DPN, respectively, demonstrated the involvement of both receptor subtypes in observed actions. Selective ERa receptor agonist, PPT, induced upregulation of both the protein level and activity of eN, while application of selective ERb receptor agonist, DPN, increased only the activity of eN. In both cases, E2 entered into the intracellular compartment and activated ER(s), which was demonstrated by membrane impermeable E2-BSA conjugate. Together these results imply that E2-induced effects on connectivity and functional properties of the hippocampal synapses may be in part mediated through observed effect on eN. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41014/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173044/RS//
dc.rightsrestrictedAccessen
dc.sourceNeuroscienceen
dc.subjectecto-5 -nucleotidase (CD73)en
dc.subject17 beta-estradiolen
dc.subjectestradiol receptors alpha and betaen
dc.subjectsynaptosomesen
dc.subjecthippocampusen
dc.title17 beta-ESTRADIOL UPREGULATES ECTO-5 -NUCLEOTIDASE (CD73) IN HIPPOCAMPAL SYNAPTOSOMES OF FEMALE RATS THROUGH ACTION MEDIATED BY ESTROGEN RECEPTOR-alpha AND -betaen
dc.typearticleen
dcterms.abstractНедељковиц, Н.; Митровић Натаса; Севигнy, Ј.; Зарић Марина; Станојловиц, М.; Дракулић Дуња; Мартиновић Јелена; Хорват, Aница; Грковић Ивана;
dc.citation.volume324
dc.citation.spage286
dc.citation.epage296
dc.identifier.wos000374145700026
dc.identifier.doi10.1016/j.neuroscience.2016.03.022
dc.citation.rankM22
dc.identifier.pmid26987957
dc.identifier.scopus2-s2.0-84962564873


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