Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location
Само за регистроване кориснике
2022
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, ...paratopes might be located in the CDRH3 or VH regions.
Кључне речи:
Antibody-antigen interaction / CDRs regions / Protein-protein interaction / VH domainИзвор:
Immunology Letters, 2022, 241, 55-57Финансирање / пројекти:
DOI: 10.1016/j.imlet.2021.11.003
ISSN: 0165-2478
WoS: 00079125450000
Scopus: 2-s2.0-85122641318
Колекције
Институција/група
VinčaTY - JOUR AU - Srdić-Rajić, Tatjana AU - Metlaš, Radmila PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10137 AB - Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, paratopes might be located in the CDRH3 or VH regions. T2 - Immunology Letters T1 - Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location VL - 241 SP - 55 EP - 57 DO - 10.1016/j.imlet.2021.11.003 ER -
@article{ author = "Srdić-Rajić, Tatjana and Metlaš, Radmila", year = "2022", abstract = "Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, paratopes might be located in the CDRH3 or VH regions.", journal = "Immunology Letters", title = "Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location", volume = "241", pages = "55-57", doi = "10.1016/j.imlet.2021.11.003" }
Srdić-Rajić, T.,& Metlaš, R.. (2022). Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location. in Immunology Letters, 241, 55-57. https://doi.org/10.1016/j.imlet.2021.11.003
Srdić-Rajić T, Metlaš R. Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location. in Immunology Letters. 2022;241:55-57. doi:10.1016/j.imlet.2021.11.003 .
Srdić-Rajić, Tatjana, Metlaš, Radmila, "Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location" in Immunology Letters, 241 (2022):55-57, https://doi.org/10.1016/j.imlet.2021.11.003 . .