TY  - JOUR
AU  - Trpković, Andreja
AU  - Todorović-Marković, Biljana
AU  - Kleut, Duška
AU  - Misirkić, Maja
AU  - Janjetović, Kristina D.
AU  - Vucicevic, Ljubica
AU  - Pantovic, Aleksandar
AU  - Jovanović, Svetlana P.
AU  - Dramićanin, Miroslav
AU  - Marković, Zoran M.
AU  - Trajković, Vladimir S.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4093
AB  - The present study investigated the hemolytic properties of fullerene (C-60) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose-and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.
T2  - Nanotechnology
T1  - Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles
VL  - 21
IS  - 37
DO  - 10.1088/0957-4484/21/37/375102
ER  - 

@article{
author = "Trpković, Andreja and Todorović-Marković, Biljana and Kleut, Duška and Misirkić, Maja and Janjetović, Kristina D. and Vucicevic, Ljubica and Pantovic, Aleksandar and Jovanović, Svetlana P. and Dramićanin, Miroslav and Marković, Zoran M. and Trajković, Vladimir S.",
year = "2010",
abstract = "The present study investigated the hemolytic properties of fullerene (C-60) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose-and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.",
journal = "Nanotechnology",
title = "Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles",
volume = "21",
number = "37",
doi = "10.1088/0957-4484/21/37/375102"
}

Trpković, A., Todorović-Marković, B., Kleut, D., Misirkić, M., Janjetović, K. D., Vucicevic, L., Pantovic, A., Jovanović, S. P., Dramićanin, M., Marković, Z. M.,& Trajković, V. S.. (2010). Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles. in Nanotechnology, 21(37).
https://doi.org/10.1088/0957-4484/21/37/375102

Trpković A, Todorović-Marković B, Kleut D, Misirkić M, Janjetović KD, Vucicevic L, Pantovic A, Jovanović SP, Dramićanin M, Marković ZM, Trajković VS. Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles. in Nanotechnology. 2010;21(37).
doi:10.1088/0957-4484/21/37/375102 .

Trpković, Andreja, Todorović-Marković, Biljana, Kleut, Duška, Misirkić, Maja, Janjetović, Kristina D., Vucicevic, Ljubica, Pantovic, Aleksandar, Jovanović, Svetlana P., Dramićanin, Miroslav, Marković, Zoran M., Trajković, Vladimir S., "Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles" in Nanotechnology, 21, no. 37 (2010),
https://doi.org/10.1088/0957-4484/21/37/375102 . .

TY  - JOUR
AU  - Jovanović, Svetlana P.
AU  - Marković, Zoran M.
AU  - Kleut, Duška
AU  - Trajković, Vladimir S.
AU  - Babić-Stojić, Branka S.
AU  - Dramićanin, Miroslav
AU  - Todorović-Marković, Biljana
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4115
AB  - In this paper, the results of the synthesis and characterization of higher fullerene-based colloids is presented. The generation of singlet oxygen (1)O2 ((1)Delta g) by fullerenc water-based colloids (nC60, nC70 and nC84) was investigated. It was found by electron paramagnetic resonance spectroscopy that the generation of singlet oxygen was the highest by the nC84 colloid. The amplitude of the electron paramagnetic resonance (EPR) signal was two orders of magnitude higher than the amplitude of the EPR signals which originated from nC60 and nC70. The surface morphology and the structure of the particles of the water-based colloids were investigated by atomic force microscopy (AFM). The AFM study showed that the average size of the nC60, nC70 and nC84 were 200, 80 and 70 nm, respectively. In addition, the particle size distribution of the nC60, nC70 and nC84 colloids was determined by dynamic light scattering (DLS) measurements.
T2  - Journal of the Serbian Chemical Society
T1  - Singlet oxygen generation by higher fullerene-based colloids
VL  - 75
IS  - 7
SP  - 965
EP  - 973
DO  - 10.2298/JSC090617062J
ER  - 

@article{
author = "Jovanović, Svetlana P. and Marković, Zoran M. and Kleut, Duška and Trajković, Vladimir S. and Babić-Stojić, Branka S. and Dramićanin, Miroslav and Todorović-Marković, Biljana",
year = "2010",
abstract = "In this paper, the results of the synthesis and characterization of higher fullerene-based colloids is presented. The generation of singlet oxygen (1)O2 ((1)Delta g) by fullerenc water-based colloids (nC60, nC70 and nC84) was investigated. It was found by electron paramagnetic resonance spectroscopy that the generation of singlet oxygen was the highest by the nC84 colloid. The amplitude of the electron paramagnetic resonance (EPR) signal was two orders of magnitude higher than the amplitude of the EPR signals which originated from nC60 and nC70. The surface morphology and the structure of the particles of the water-based colloids were investigated by atomic force microscopy (AFM). The AFM study showed that the average size of the nC60, nC70 and nC84 were 200, 80 and 70 nm, respectively. In addition, the particle size distribution of the nC60, nC70 and nC84 colloids was determined by dynamic light scattering (DLS) measurements.",
journal = "Journal of the Serbian Chemical Society",
title = "Singlet oxygen generation by higher fullerene-based colloids",
volume = "75",
number = "7",
pages = "965-973",
doi = "10.2298/JSC090617062J"
}

Jovanović, S. P., Marković, Z. M., Kleut, D., Trajković, V. S., Babić-Stojić, B. S., Dramićanin, M.,& Todorović-Marković, B.. (2010). Singlet oxygen generation by higher fullerene-based colloids. in Journal of the Serbian Chemical Society, 75(7), 965-973.
https://doi.org/10.2298/JSC090617062J

Jovanović SP, Marković ZM, Kleut D, Trajković VS, Babić-Stojić BS, Dramićanin M, Todorović-Marković B. Singlet oxygen generation by higher fullerene-based colloids. in Journal of the Serbian Chemical Society. 2010;75(7):965-973.
doi:10.2298/JSC090617062J .

Jovanović, Svetlana P., Marković, Zoran M., Kleut, Duška, Trajković, Vladimir S., Babić-Stojić, Branka S., Dramićanin, Miroslav, Todorović-Marković, Biljana, "Singlet oxygen generation by higher fullerene-based colloids" in Journal of the Serbian Chemical Society, 75, no. 7 (2010):965-973,
https://doi.org/10.2298/JSC090617062J . .