TY  - JOUR
AU  - Vranješ, Sanja
AU  - Jovanović, Moma S.
AU  - Prvulović, M.
AU  - Stefanović, L.
AU  - Kljajić, R.
AU  - Ratajac, R.
PY  - 2002
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2526
AB  - On the base of property to enter into myocardial cells as a calcium channel blocker, verapamil was labeled with technetium-99m in order to investigate the possibility to obtain new radiopharmaceutical for myocardial imaging. The conditions of labeling verapamil with technetium-99m for different ammounts of stannous(II) ion, mannitol, cystein and pH range 2.5-3.5 were examined. Investigation of radiochemical purity ( GT 95%) and biodistribution of (9)9mTc-verapamil in rats showed that it was stable during 2 hours after labeling. Accumulation of Tc-99m-verapamil in heart was 1.2% and in liver 9.4%, 5 minutes after injection. Biodistribution of Tc-99m-verapamil in rats in conditions of stress, pharmacologically caused by dipiridamol, showed that the elimination of Tc-99m-verapamil from the heart was slower related to the control group. In the group of rats previously treated with isoproterenol uptake of Tc-99m-verapamil in heart was lower related to the control group (0.7% versus 1.0%) 5 minutes after injection. Lipophilicity of (9)9mTc-verapamil was examined by determination of partition coefficient (log P = 0.62) and protein binding (79%). Imaging studies on dogs provided relatively good myocardial images with partially overlap of activity in the lung and liver.
T2  - Journal of Radioanalytical and Nuclear Chemistry
T1  - Chemical and biological properties of verapamil labeled with technetium-99m: A potential myocardial imaging agents
VL  - 253
IS  - 1
SP  - 81
EP  - 86
DO  - 10.1023/A:1015864501284
ER  - 

@article{
author = "Vranješ, Sanja and Jovanović, Moma S. and Prvulović, M. and Stefanović, L. and Kljajić, R. and Ratajac, R.",
year = "2002",
abstract = "On the base of property to enter into myocardial cells as a calcium channel blocker, verapamil was labeled with technetium-99m in order to investigate the possibility to obtain new radiopharmaceutical for myocardial imaging. The conditions of labeling verapamil with technetium-99m for different ammounts of stannous(II) ion, mannitol, cystein and pH range 2.5-3.5 were examined. Investigation of radiochemical purity ( GT 95%) and biodistribution of (9)9mTc-verapamil in rats showed that it was stable during 2 hours after labeling. Accumulation of Tc-99m-verapamil in heart was 1.2% and in liver 9.4%, 5 minutes after injection. Biodistribution of Tc-99m-verapamil in rats in conditions of stress, pharmacologically caused by dipiridamol, showed that the elimination of Tc-99m-verapamil from the heart was slower related to the control group. In the group of rats previously treated with isoproterenol uptake of Tc-99m-verapamil in heart was lower related to the control group (0.7% versus 1.0%) 5 minutes after injection. Lipophilicity of (9)9mTc-verapamil was examined by determination of partition coefficient (log P = 0.62) and protein binding (79%). Imaging studies on dogs provided relatively good myocardial images with partially overlap of activity in the lung and liver.",
journal = "Journal of Radioanalytical and Nuclear Chemistry",
title = "Chemical and biological properties of verapamil labeled with technetium-99m: A potential myocardial imaging agents",
volume = "253",
number = "1",
pages = "81-86",
doi = "10.1023/A:1015864501284"
}

Vranješ, S., Jovanović, M. S., Prvulović, M., Stefanović, L., Kljajić, R.,& Ratajac, R.. (2002). Chemical and biological properties of verapamil labeled with technetium-99m: A potential myocardial imaging agents. in Journal of Radioanalytical and Nuclear Chemistry, 253(1), 81-86.
https://doi.org/10.1023/A:1015864501284

Vranješ S, Jovanović MS, Prvulović M, Stefanović L, Kljajić R, Ratajac R. Chemical and biological properties of verapamil labeled with technetium-99m: A potential myocardial imaging agents. in Journal of Radioanalytical and Nuclear Chemistry. 2002;253(1):81-86.
doi:10.1023/A:1015864501284 .

Vranješ, Sanja, Jovanović, Moma S., Prvulović, M., Stefanović, L., Kljajić, R., Ratajac, R., "Chemical and biological properties of verapamil labeled with technetium-99m: A potential myocardial imaging agents" in Journal of Radioanalytical and Nuclear Chemistry, 253, no. 1 (2002):81-86,
https://doi.org/10.1023/A:1015864501284 . .