@conference{
author = "Vasiljević, N. and Veljković, Nevena V. and Jovanović-Ćupić, Snežana P. and Glišić, Sanja",
year = "2010",
abstract = "Background:Numerous studies have reported a relationship betweenhepatitis C virus (HCV) genotype and the response to interferon therapy.Despite high sensitivity and specificity, genotyping methods can be per-formed only on HCV RNA positive samples. Serotyping might be a rapidand cost effective method for determining HCV genotypes, especially inpatients with previously undetectable HCV RNA.Aim:The aim of this study was to select NS5A-derived peptides whichmay represent candidate specific markers for determination of HCVgenotypes by serotyping.Methods:The conserved structural and informational properties of HCVNS5A protein have been determined by the informational spectrum method(ISM), representing virtual spectroscopy method for analysis of relationshipbetween structure and function of proteins.Results:The ISM analysis revealed that NS5A proteins from HCV geno-types 1,2,3,4 and 6 encode the conserved information which is specific foreasch particular genotype, and which is represented by IS frequenciesF1(0.258), F2(0.271), F3(0.053), F4(0.205) and F6(0.146), respectively. TheNS5-derived peptides P1(120-152), P2(383-417), P3(405-439), P4(171-205), P6(374-408) are identified as domains that are essential for conservedinformation characterizing HCV genotypes 1, 2, 3, 4 and 6.Conclusions:Peptides P1, P2, P3, P4 and P6 derived from NS5A proteinrepresent candidate markers for simple and fast identification of HCVgenotypes by serotyping",
journal = "Vox Sanguinis",
title = "Selection of Ns5a-Derived Peptides for Determination of Hcv Genotypes By Serotyping",
volume = "99",
number = "Supp: 1",
pages = "299",
doi = "10.1111/j.1423-0410.2010.01343_2.x",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4043"
}